Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 1088403
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide

Specificity

Detects a synthetic peptide specific for mouse Ki-67 around amino acid 1185 in Direct ELISA. 

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Ki67/MKI67 Antibody

Detection of Ki67/MKI67 in Mouse Colon.

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse Ki67/MKI67 Monoclonal Antibody (Catalog # MAB11612) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Ki67/MKI67 in Raw264.7.

Ki67/MKI67 was detected in immersion fixed RAW264.7 cells using Mouse Anti-Mouse Ki67/MKI67 Monoclonal Antibody (Catalog # MAB11612) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Ki67/MKI67 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed RAW264.7 cells

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Colon

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ki67/MKI67

MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053‑3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Entrez Gene IDs

4288 (Human); 17345 (Mouse); 291234 (Rat)

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Mouse Ki67/MKI67 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Ki67/MKI67 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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