Mouse Leptin/OB Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF498
Product Details
Citations (2)
FAQs
Supplemental Products
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Mouse Leptin/OB Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin/OB in ELISAs and Western blots. In sandwich immunoassays, less than 45% cross-reactivity with recombinant rat (rr) Leptin is observed and less than 0.2% cross-reactivity with rhLeptin and rmLeptin R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Leptin (R&D Systems, Catalog # 498-OB)
Val22-Cys167
Accession # Q544U0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin/OB (Catalog # 498-OB)

Mouse Leptin/OB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Leptin/OB Antibody (Catalog # AF498)

Standard: Recombinant Mouse Leptin Protein, CF (Catalog # 498-OB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citations for Mouse Leptin/OB Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Engineering a pharmacologically superior form of leptin for the treatment of obesity.
    Authors: Lo KM, Morelli B, Lan Y, Lauder S, Brunkhorst B, Webster G, Hallakou-Bozec S, Doare L, Gillies SD
    Protein Eng. Des. Sel., 2005-01-01;18(1):1-10.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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