Mouse Lipocalin-2/NGAL Quantikine ELISA Kit

  (20 citations)
(1 Review)
    
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Assay Procedure
Citations (20)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL)
  • Sensitivity
    8.8 pg/mL
  • Assay Range
    78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant mouse Lipocalin-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 196 476 1534 211 477 1564
Standard Deviation 13.2 19.6 65.9 15.2 28.6 98.5
CV% 6.7 4.1 4.3 7.2 6 6.3

Recovery

The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 93 82-101
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.
 Lipocalin-2/NGAL [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Lipocalin-2/NGAL
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
  • Long Name:
    Neutrophil Gelatinase-associated Lipocalin
  • Entrez Gene IDs:
    3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
  • Alternate Names:
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Sample Type
  1. Human Alpha-1-Antitrypsin (hAAT) therapy reduces renal dysfunction and acute tubular necrosis in a murine model of bilateral kidney ischemia-reperfusion injury
    Authors: N Maicas, J van der Vl, J Bublitz, S Florquin, M Bakker-van, CA Dinarello, V Verweij, R Masereeuw, LA Joosten, LB Hilbrands
    PLoS ONE, 2017;12(2):e0168981.
    Species: Mouse
    Sample Type: Plasma
  2. Autophagy Inhibits the Accumulation of Advanced Glycation End Products by Promoting Lysosomal Biogenesis and Function in the Kidney Proximal Tubules
    Authors: A Takahashi, Y Takabatake, T Kimura, I Maejima, T Namba, T Yamamoto, J Matsuda, S Minami, JY Kaimori, I Matsui, T Matsusaka, F Niimura, T Yoshimori, Y Isaka
    Diabetes, 2017;0(0):.
    Species: Mouse
    Sample Type: Urine
  3. Ameliorative Effect of Daidzein on Cisplatin-Induced Nephrotoxicity in Mice via Modulation of Inflammation, Oxidative Stress, and Cell Death
    Authors: H Meng, G Fu, J Shen, K Shen, Z Xu, Y Wang, B Jin, H Pan
    Oxid Med Cell Longev, 2017;2017(0):3140680.
    Species: Mouse
    Sample Type: Serum
  4. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
  5. CD40-signalling abrogates induction of ROR?t(+) Treg cells by intestinal CD103(+) DCs and causes fatal colitis
    Authors: C Barthels, A Ogrinc, V Steyer, S Meier, F Simon, M Wimmer, A Blutke, T Straub, U Zimber-Str, E Lutgens, P Marconi, C Ohnmacht, D Garzetti, B Stecher, T Brocker
    Nat Commun, 2017;8(0):14715.
    Species: Mouse
    Sample Type: Tissue Homogenates
  6. Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules
    Sci Rep, 2016;6(0):36533.
    Species: Mouse
    Sample Type: Urine
  7. The effects of exogenous desmopressin on a model of heat stress nephropathy in mice
    Authors: Carlos A Roncal-Jim
    Am. J. Physiol. Renal Physiol, 2016;0(0):ajprenal.0049.
    Species: Mouse
    Sample Type: Urine
  8. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation
    Authors: F Aigner
    Am. J. Transplant., 2016;16(3):808-20.
    Species: Mouse
    Sample Type: Serum
  9. Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure
    Authors: Tanu Parmar
    Invest Ophthalmol Vis Sci, 2016;57(7):3257-67.
    Species: Mouse
    Sample Type: Cell Lysates
  10. Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice
    Sci Rep, 2016;6(0):30887.
    Species: Mouse
    Sample Type: Feces
  11. Renal Impairment with Sublethal Tubular Cell Injury in a Chronic Liver Disease Mouse Model.
    Authors: Ishida T, Kotani H, Miyao M, Kawai C, Jemail L, Abiru H, Tamaki K
    PLoS ONE, 2016;11(1):e0146871.
    Species: Mouse
    Sample Type: Urine
  12. Lipocalin-2 promotes m1 macrophages polarization in a mouse cardiac ischaemia-reperfusion injury model.
    Authors: Cheng L, Xing H, Mao X, Li L, Li X, Li Q
    Scand J Immunol, 2015;81(1):31-8.
    Species: Mouse
    Sample Type: Serum
  13. The small fibrinopeptide Bbeta15-42 as renoprotective agent preserving the endothelial and vascular integrity in early ischemia reperfusion injury in the mouse kidney.
    Authors: Urbschat A, Zacharowski K, Obermuller N, Rupprecht K, Penzkofer D, Jennewein C, Tran N, Scheller B, Dimmeler S, Paulus P
    PLoS ONE, 2014;9(1):e84432.
    Species: Mouse
    Sample Type: Serum
  14. Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice.
    Authors: Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H
    PLoS ONE, 2014;9(4):e94373.
    Species: Mouse
    Sample Type: Urine
  15. Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
    Authors: Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H
    Nat Commun, 2014;5(0):4088.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  16. Lipocalin2 as a plasma marker for tumors with hypoxic regions.
    Authors: Nakamura I, Hama S, Itakura S, Takasaki I, Nishi T, Tabuchi Y, Kogure K
    Sci Rep, 2014;4(0):7235.
    Species: Mouse
    Sample Type: Plasma
  17. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies.
    Authors: Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S
    PLoS ONE, 2013;8(11):e79037.
    Species: Mouse
    Sample Type: Urine
  18. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2012;80(1):410-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
  19. ATP release and autocrine signaling through P2X4 receptors regulate gammadelta T cell activation.
    Authors: Manohar M, Hirsh M, Chen Y, Woehrle T, Karande A, Junger W
    J Leukoc Biol, 2012;92(4):787-94.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  20. Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
    Authors: Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G
    Blood, 2009;114(17):3642-51.
    Species: Mouse
    Sample Type: Serum
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