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Mouse Lipocalin-2/NGAL ELISA Kit - Quantikine

Catalog #: MLCN20
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Mouse Lipocalin-2/NGAL ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
8.8 pg/mL
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse Lipocalin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Platelet-poor Plasma/Urine - Samples were evaluated for the presence of mouse Lipocalin-2 in this assay. 

Sample Type Mean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=20)14158.3-29060.2
Platelet-poor heparin plasma (n=20)76.040.7-14330.1
Platelet-poor EDTA plasma (n=20)54.132.5-74.712.3
Urine (n=20)48.612.9-16834.1

Cell Culture Supernates:
J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. Cells were either untreated or stimulated with 100 ng/mL of recombinant mouse IFN-gamma and 1 μg/mL of lipopolysaccharide (LPS) for 2 days. Aliquots of the cell culture supernates were removed and assayed for levels of mouse Lipocalin-2.

ConditionObserved Values (ng/mL)
Unstimulated0.817
Stimulated161

WEHI-3 myelomonocytic leukemia cells (2 x 105 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. Cells were either untreated or stimulated with 1 μg/mL of LPS for 4 days. Aliquots of the cell culture supernates were removed and assayed for levels of mouse Lipocalin-2.

ConditionObserved Values (ng/mL)
Unstimulated83.7
Stimulated178

Two mouse brains and kidneys were homogenized and seeded in RPMI 1640 supplemented with 10% fetal bovine serum, 5 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. The resulting supernates were either untreated or stimulated with 1 μg/mL of LPS for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of mouse Lipocalin-2.

ConditionObserved Values (ng/mL)
Brain unstimulated0.334
Brain stimulated178

ConditionObserved Values (ng/mL)
Kidney Unstimulated1.28
Kidney Stimulated4.69





Product Summary

The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 196 476 1534 211 477 1564
Standard Deviation 13.2 19.6 65.9 15.2 28.6 98.5
CV% 6.7 4.1 4.3 7.2 6 6.3

Recovery

The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 93 82-101

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.
Mouse Lipocalin-2/NGAL ELISA Linearity

Scientific Data

N/A Mouse Lipocalin-2/NGAL ELISA Standard Curve View Larger

Mouse Lipocalin-2/NGAL ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs:
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names:
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

65 Citations: Showing 1 - 10
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  1. Antidepressant Effects of Ginsenoside Rc on L-Alpha-Aminoadipic Acid-Induced Astrocytic Ablation and Neuroinflammation in Mice
    Authors: Kwon, D;Kim, Y;Cho, SH;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Serum and urinary biomarkers of vancomycin-induced acute kidney injury: A prospective, observational analysis
    Authors: Park, SI;Yu, U;Oh, WS;Ryu, SW;Son, S;Lee, S;Baek, H;Park, JI;
    Medicine
    Species: Human
    Sample Types: Urine
  3. Activation of the osteoblastic HIF-1? pathway partially alleviates the symptoms of STZ-induced type 1 diabetes mellitus via RegIII?
    Authors: Qiu, M;Chang, L;Tang, G;Ye, W;Xu, Y;Tulufu, N;Dan, Z;Qi, J;Deng, L;Li, C;
    Experimental & molecular medicine
    Species: Transgenic Mouse
    Sample Types: Serum
  4. Antidepressant Effect of Heracleum moellendorffii Extract on Behavioral Changes in Astrocyte Ablation Mouse Model of Depression by Modulating Neuroinflammation through the Inhibition of Lipocalin-2
    Authors: Hong, S;Kim, Y;Kwon, Y;Cho, SH;
    Nutrients
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Diet-induced metabolic and immune impairments are sex-specifically modulated by soluble TNF signaling in the 5xFAD mouse model of Alzheimer's disease
    Authors: Maria Elizabeth De Sousa Rodrigues, MacKenzie L Bolen, Lisa Blackmer-Raynolds, Noah Schwartz, Jianjun Chang, Malú Gámez Tansey, Timothy Robert Sampson
    Neurobiol Dis
    Species: Transgenic Mouse
    Sample Types: Plasma
  6. Lipocalin-2 as a prognostic marker in patients with acute exacerbation of idiopathic pulmonary fibrosis
    Authors: Tanahashi, H;Iwamoto, H;Yamaguchi, K;Sakamoto, S;Horimasu, Y;Masuda, T;Nakashima, T;Ohshimo, S;Fujitaka, K;Hamada, H;Hattori, N;
    Respiratory research
    Species: Mouse
    Sample Types: Serum
  7. Mesenchymal Stem Cells Delivered Locally to Ischemia-Reperfused Kidneys via Injectable Hyaluronic Acid Hydrogels Decrease Extracellular Matrix Remodeling 1 Month after Injury in Male Mice
    Authors: Han, DS;Erickson, C;Hansen, KC;Kirkbride-Romeo, L;He, Z;Rodell, CB;Soranno, DE;
    Cells
    Species: Mouse
    Sample Types: Urine
  8. IL-17C neutralization protects the kidney against acute injury and chronic injury
    Authors: Zhang, F;Yin, J;Liu, L;Liu, S;Zhang, G;Kong, Y;Wang, Y;Wang, N;Chen, X;Wang, F;
    EBioMedicine
    Species: Mouse
    Sample Types: Serum
  9. T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade
    Authors: Lee, K;Thompson, EA;Gharaie, S;Patel, CH;Kurzhagen, JT;Pierorazio, PM;Arend, LJ;Thomas, AG;Noel, S;Slusher, BS;Rabb, H;
    JCI insight
    Species: Mouse
    Sample Types: Plasma
  10. Soluble TNF mediates amyloid-independent, diet-induced alterations to immune and neuronal functions in an Alzheimer's disease mouse model
    Authors: KP MacPherson, LN Eidson, MC Houser, BE Weiss, JL Gollihue, MK Herrick, ME de Sousa R, L Sniffen, EM Weekman, AM Hamilton, SD Kelly, DL Oliver, Y Yang, J Chang, TR Sampson, CM Norris, MG Tansey
    Frontiers in Cellular Neuroscience, 2023-03-15;17(0):895017.
    Species: Mouse
    Sample Types: Plasma
  11. Glutamine prevents acute kidney injury by modulating oxidative stress and apoptosis in tubular epithelial cells
    Authors: K Thomas, L Zondler, N Ludwig, M Kardell, C Lüneburg, K Henke, S Mersmann, A Margraf, T Spieker, T Tekath, A Velic, R Holtmeier, J Hermann, V Jankowski, M Meersch, D Vestweber, M Westphal, J Roth, MA Schäfers, JA Kellum, CA Lowell, J Rossaint, A Zarbock
    JCI Insight, 2022-11-08;7(21):.
    Species: Mouse
    Sample Types: Urine
  12. Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease model
    Authors: K Tange, S Yagi, E Takeshita, M Abe, Y Yamamoto, H Tomida, T Kawamura, M Hanayama, B Matsuura, Y Ikeda, Y Hiasa
    Scientific Reports, 2022-10-26;12(1):17983.
    Species: Mouse
    Sample Types: Feces
  13. Immune-mediated tubule atrophy promotes acute kidney injury to chronic kidney disease transition
    Authors: L Xu, J Guo, DG Moledina, LG Cantley
    Nature Communications, 2022-08-19;13(1):4892.
    Species: Mouse
    Sample Types: Serum
  14. Effects of Suramin on Polycystic Kidney Disease in a Mouse Model of Polycystin-1 Deficiency
    Authors: MY Chang, SH Hsu, LY Ma, LF Chou, CC Hung, YC Tian, CW Yang
    International Journal of Molecular Sciences, 2022-07-31;23(15):.
    Species: Mouse
    Sample Types: Plasma
  15. Maternal heme-enriched diet promotes a gut pro-oxidative status associated with microbiota alteration, gut leakiness and glucose intolerance in mice offspring
    Authors: A Mazenc, L Mervant, C Maslo, C Lencina, V Bézirard, M Levêque, I Ahn, V Alquier-Ba, N Naud, C Héliès-Tou, L Debrauwer, S Chevolleau, F Guéraud, FHF Pierre, V Théodorou, M Olier
    Redox Biology, 2022-05-12;53(0):102333.
    Species: Mouse
    Sample Types: Feces
  16. Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia
    Authors: X Xiang, X Tang, Y Yu, S Xie, L Liu, M Chen, R Zhang, X Kang, Y Zheng, G Yang, S Gan, S Zhu
    Journal of Neuroinflammation, 2022-04-12;19(1):92.
    Species: Mouse
    Sample Types: Serum
  17. Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury
    Authors: TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch
    JCI Insight, 2022-03-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells
    Authors: TH Chen, CT Liu, CY Cheng, YM Sue, NJ Huang, CH Chen
    Nutrients, 2022-02-11;14(4):.
    Species: Rat
    Sample Types: Serum
  19. Resident Self-Tissue of Proinflammatory Cytokines Rather Than Their Systemic Levels Correlates with Development of Myelofibrosis in Gata1low Mice
    Authors: M Zingariell, P Verachi, F Gobbo, F Martelli, M Falchi, M Mazzarini, M Valeri, G Sarli, C Marinaccio, J Melo-Carde, JD Crispino, AR Migliaccio
    Biomolecules, 2022-01-30;12(2):.
    Species: Mouse
    Sample Types: Serum
  20. MicroRNA?93 inhibits the apoptosis and inflammatory response of tubular epithelial cells via the PTEN/AKT/mTOR pathway in acute kidney injury
    Authors: Y Zhan, M Zhu, S Liu, J Lu, Z Ni, H Cai, W Zhang
    Molecular Medicine Reports, 2021-07-23;24(3):.
    Species: Mouse
    Sample Types: Serum
  21. Gut microbiome-host interactions in driving environmental pollutant trichloroethene-mediated autoimmunity
    Authors: H Wang, N Banerjee, Y Liang, G Wang, KL Hoffman, MF Khan
    Toxicology and Applied Pharmacology, 2021-05-27;424(0):115597.
    Species: Mouse
    Sample Types: Serum
  22. Angiotensin AT2 Receptor is Anti-inflammatory and Reno-Protective in Lipopolysaccharide Mice Model: Role of IL-10
    Authors: N Fatima, S Patel, T Hussain
    Frontiers in Pharmacology, 2021-04-15;12(0):600163.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon
    Authors: A Nakamura, S Kurihara, D Takahashi, W Ohashi, Y Nakamura, S Kimura, M Onuki, A Kume, Y Sasazawa, Y Furusawa, Y Obata, S Fukuda, S Saiki, M Matsumoto, K Hase
    Nature Communications, 2021-04-08;12(1):2105.
    Species: Mouse
    Sample Types: Faecal supernates
  24. Effects of selective TNFR1 inhibition or TNFR2 stimulation, compared to non-selective TNF inhibition, on (neuro)inflammation and behavior after myocardial infarction in male mice
    Authors: L Gouweleeuw, H Wajant, O Maier, ULM Eisel, WM Blankestei, RG Schoemaker
    Brain, Behavior, and Immunity, 2021-01-12;0(0):.
    Species: Mouse
    Sample Types: Plasma
  25. Conditional Myh9 and Myh10 inactivation in adult mouse renal epithelium results in progressive kidney disease
    Authors: KL Otterpohl, BW Busselman, I Ratnayake, RG Hart, K Hart, C Evans, CL Phillips, JR Beach, P Ahrenkiel, B Molitoris, K Surendran, I Chandrasek
    JCI Insight, 2020-11-05;0(0):.
    Species: Mouse
    Sample Types: Serum
  26. LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1
    Authors: JY Yoo, DR Cha, B Kim, EJ An, SR Lee, JJ Cha, YS Kang, JY Ghee, JY Han, YS Bae
    Cell Rep, 2020-10-20;33(3):108245.
    Species: Mouse
    Sample Types: Serum
  27. Neutralization of Lipocalin-2 Diminishes Stroke-Reperfusion Injury
    Authors: Guona Wang, Yi-Chinn Weng, I-Chen Chiang, Yu-Ting Huang, Yi-Chu Liao, Yi-Chun Chen et al.
    International Journal of Molecular Sciences
    Species: Transgenic Mouse
    Sample Types: Serum, Tissue Homogenates
  28. Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
    Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
    Microorganisms, 2020-06-04;8(6):.
    Species: Mouse
    Sample Types: Fecal Supernates
  29. Sodium-glucose cotransporter 2 inhibition suppresses HIF-1&alpha-mediated metabolic switch from lipid oxidation to glycolysis in kidney tubule cells of diabetic mice
    Authors: T Cai, Q Ke, Y Fang, P Wen, H Chen, Q Yuan, J Luo, Y Zhang, Q Sun, Y Lv, K Zen, L Jiang, Y Zhou, J Yang
    Cell Death Dis, 2020-05-22;11(5):390.
    Species: Human
    Sample Types: Urine
  30. Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis
    Authors: SA Tanusepute, MT Lin, SL Yeh, CL Yeh
    Mediators Inflamm., 2020-05-11;2020(0):3201635.
    Species: Mouse
    Sample Types: Plasma
  31. Lipocalin2 Induced by Bacterial Flagellin Protects Mice against Cyclophosphamide Mediated Neutropenic Sepsis
    Authors: D Lim, HK Kim, JH Jeong, YS Jung, SE Lee, HC Jang, SI Jung, HS Choi, JH Rhee, SG Lee, C Park, M Song, HE Choy
    Microorganisms, 2020-04-29;8(5):.
    Species: Mouse
    Sample Types: Serum
  32. A Novel Approach to Deliver Therapeutic Extracellular Vesicles Directly into the Mouse Kidney via Its Arterial Blood Supply
    Authors: M Ullah, DD Liu, S Rai, M Razavi, J Choi, J Wang, W Concepcion, AS Thakor
    Cells, 2020-04-10;9(4):.
    Species: Mouse
    Sample Types: Serum
  33. CXCL14 Overexpression Attenuates Sepsis-Associated Acute Kidney Injury by Inhibiting Proinflammatory Cytokine Production
    Authors: J Lv, ZL Wu, Z Gan, P Gui, SL Yao
    Mediators Inflamm., 2020-03-31;2020(0):2431705.
    Species: Mouse
    Sample Types: Tissue Homogenate
  34. Targeting soluble tumor necrosis factor as a potential intervention to lower risk for late-onset Alzheimer's disease associated with obesity, metabolic syndrome, and type 2 diabetes
    Authors: ME De Sousa R, MC Houser, DI Walker, DP Jones, J Chang, CJ Barnum, MG Tansey
    Alzheimers Res Ther, 2019-12-31;12(1):1.
    Species: Mouse
    Sample Types: Plasma
  35. Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking
    Authors: L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini
    Cell Rep, 2019-11-12;29(7):2067-2077.e6.
    Species: Mouse
    Sample Types: cell culture supernatant
  36. Discovery and preclinical evaluation of anti-miR-17 oligonucleotide RGLS4326 for the treatment of polycystic kidney disease
    Authors: EC Lee, T Valencia, C Allerson, A Schairer, A Flaten, M Yheskel, K Kersjes, J Li, S Gatto, M Takhar, S Lockton, A Pavlicek, M Kim, T Chu, R Soriano, S Davis, JR Androsavic, S Sarwary, T Owen, J Kaplan, K Liu, G Jang, S Neben, P Bentley, T Wright, V Patel
    Nat Commun, 2019-09-12;10(1):4148.
    Species: Mouse
    Sample Types: Urine
  37. Lipocalin-2 (Lcn-2) Attenuates Polymicrobial Sepsis with LPS Preconditioning (LPS Tolerance) in FcGRIIb Deficient Lupus Mice
    Authors: T Ondee, J Gillen, P Visitchana, P Somparn, J Issara-Amp, C Dang Phi, W Chancharoe, D Gurusamy, A Nita-Lazar, A Leelahavan
    Cells, 2019-09-11;8(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Modulation of the lung inflammatory response to ozone by the estrous cycle
    Authors: N Fuentes, N Cabello, M Nicoleau, ZC Chroneos, P Silveyra
    Physiol Rep, 2019-03-01;7(5):e14026.
    Species: Mouse
    Sample Types: BALF
  39. Negr1 controls adult hippocampal neurogenesis and affective behaviors
    Authors: K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee
    Mol. Psychiatry, 2019-01-16;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  40. NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions
    Authors: N Makhezer, M Ben Khemis, D Liu, Y Khichane, V Marzaioli, A Tlili, M Mojallali, C Pintard, P Letteron, M Hurtado-Ne, J El-Benna, JC Marie, A Sannier, AL Pelletier, PM Dang
    Mucosal Immunol, 2018-10-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  41. Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer
    Authors: J Steenbrugg, K Breyne, K Demeyere, O De Wever, NN Sanders, W Van Den Br, C Colpaert, P Vermeulen, S Van Laere, E Meyer
    J. Exp. Clin. Cancer Res., 2018-08-15;37(1):191.
    Species: Mouse
    Sample Types: Serum
  42. Effects of lobeglitazone on insulin resistance and hepatic steatosis in high-fat diet-fed mice
    Authors: BH Choi, Z Jin, CO Yi, J Oh, EA Jeong, JY Lee, KA Park, KE Kim, JE Lee, HJ Kim, JR Hahm, GS Roh
    PLoS ONE, 2018-07-06;13(7):e0200336.
    Species: Mouse
    Sample Types: Serum
  43. RIPK3 promotes sepsis-induced acute kidney injury via mitochondrial dysfunction
    Authors: A Sureshbabu, E Patino, KC Ma, K Laursen, EJ Finkelszte, O Akchurin, T Muthukumar, SW Ryter, L Gudas, AMK Choi, ME Choi
    JCI Insight, 2018-06-07;3(11):.
    Species: Mouse
    Sample Types: Urine
  44. Endothelium-targeted delivery of dexamethasone by anti-VCAM-1 SAINT-O-Somes in mouse endotoxemia
    Authors: R Li, PS Kowalski, HWM Morselt, I Schepel, RM Jongman, A Aslan, MHJ Ruiters, JG Zijlstra, G Molema, M van Meurs, JAAM Kamps
    PLoS ONE, 2018-05-15;13(5):e0196976.
    Species: Mouse
    Sample Types: Plasma
  45. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity
    Authors: JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino
    Mol Ther Nucleic Acids, 2017-11-24;10(0):103-121.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  46. Ameliorative Effect of Daidzein on Cisplatin-Induced Nephrotoxicity in Mice via Modulation of Inflammation, Oxidative Stress, and Cell Death
    Authors: H Meng, G Fu, J Shen, K Shen, Z Xu, Y Wang, B Jin, H Pan
    Oxid Med Cell Longev, 2017-08-02;2017(0):3140680.
    Species: Mouse
    Sample Types: Serum
  47. CD40-signalling abrogates induction of ROR?t(+) Treg cells by intestinal CD103(+) DCs and causes fatal colitis
    Authors: C Barthels, A Ogrinc, V Steyer, S Meier, F Simon, M Wimmer, A Blutke, T Straub, U Zimber-Str, E Lutgens, P Marconi, C Ohnmacht, D Garzetti, B Stecher, T Brocker
    Nat Commun, 2017-03-09;8(0):14715.
    Species: Mouse
    Sample Types: Tissue Homogenates
  48. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  49. Autophagy Inhibits the Accumulation of Advanced Glycation End Products by Promoting Lysosomal Biogenesis and Function in the Kidney Proximal Tubules
    Authors: A Takahashi, Y Takabatake, T Kimura, I Maejima, T Namba, T Yamamoto, J Matsuda, S Minami, JY Kaimori, I Matsui, T Matsusaka, F Niimura, T Yoshimori, Y Isaka
    Diabetes, 2017-02-28;0(0):.
    Species: Mouse
    Sample Types: Urine
  50. Human Alpha-1-Antitrypsin (hAAT) therapy reduces renal dysfunction and acute tubular necrosis in a murine model of bilateral kidney ischemia-reperfusion injury
    Authors: N Maicas, J van der Vl, J Bublitz, S Florquin, M Bakker-van, CA Dinarello, V Verweij, R Masereeuw, LA Joosten, LB Hilbrands
    PLoS ONE, 2017-02-24;12(2):e0168981.
    Species: Mouse
    Sample Types: Plasma
  51. The effects of exogenous desmopressin on a model of heat stress nephropathy in mice
    Authors: Carlos A Roncal-Jim
    Am. J. Physiol. Renal Physiol, 2016-12-21;0(0):ajprenal.0049.
    Species: Mouse
    Sample Types: Urine
  52. Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules
    Sci Rep, 2016-11-09;6(0):36533.
    Species: Mouse
    Sample Types: Urine
  53. Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice
    Sci Rep, 2016-07-29;6(0):30887.
    Species: Mouse
    Sample Types: Feces
  54. Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure
    Authors: Tanu Parmar
    Invest Ophthalmol Vis Sci, 2016-06-01;57(7):3257-67.
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
  55. Renal Impairment with Sublethal Tubular Cell Injury in a Chronic Liver Disease Mouse Model.
    Authors: Ishida T, Kotani H, Miyao M, Kawai C, Jemail L, Abiru H, Tamaki K
    PLoS ONE, 2016-01-11;11(1):e0146871.
    Species: Mouse
    Sample Types: Urine
  56. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation
    Authors: F Aigner
    Am. J. Transplant., 2015-11-23;16(3):808-20.
    Species: Mouse
    Sample Types: Serum
  57. Lipocalin-2 promotes m1 macrophages polarization in a mouse cardiac ischaemia-reperfusion injury model.
    Authors: Cheng L, Xing H, Mao X, Li L, Li X, Li Q
    Scand J Immunol, 2015-01-01;81(1):31-8.
    Species: Mouse
    Sample Types: Serum
  58. Lipocalin2 as a plasma marker for tumors with hypoxic regions.
    Authors: Nakamura I, Hama S, Itakura S, Takasaki I, Nishi T, Tabuchi Y, Kogure K
    Sci Rep, 2014-12-03;4(0):7235.
    Species: Mouse
    Sample Types: Plasma
  59. Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
    Authors: Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H
    Nat Commun, 2014-06-09;5(0):4088.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  60. Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice.
    Authors: Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H
    PLoS ONE, 2014-04-18;9(4):e94373.
    Species: Mouse
    Sample Types: Urine
  61. The small fibrinopeptide Bbeta15-42 as renoprotective agent preserving the endothelial and vascular integrity in early ischemia reperfusion injury in the mouse kidney.
    Authors: Urbschat A, Zacharowski K, Obermuller N, Rupprecht K, Penzkofer D, Jennewein C, Tran N, Scheller B, Dimmeler S, Paulus P
    PLoS ONE, 2014-01-02;9(1):e84432.
    Species: Mouse
    Sample Types: Serum
  62. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies.
    Authors: Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S
    PLoS ONE, 2013-11-12;8(11):e79037.
    Species: Mouse
    Sample Types: Urine
  63. ATP release and autocrine signaling through P2X4 receptors regulate gammadelta T cell activation.
    Authors: Manohar M, Hirsh M, Chen Y, Woehrle T, Karande A, Junger W
    J Leukoc Biol, 2012-06-29;92(4):787-94.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  64. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2011-10-28;80(1):410-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
    Authors: Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G
    Blood, 2009-08-21;114(17):3642-51.
    Species: Mouse
    Sample Types: Serum

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Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 12/05/2024
Sample Tested: Serum

Wide dynamic range. Easy to use kit.

Mouse Lipocalin-2/NGAL Quantikine ELISA Kit MLCN20
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 12/11/2021
Sample Tested: Serum
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit MLCN20
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 02/10/2021
Sample Tested: Kidney tissue,Urine
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit MLCN20
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
By Nicole Brown on 01/07/2019
Sample Tested: Serum

Wild type (WT) or IL33-/- (Def) mice were given either vehicle (Veh) or cisplatin (Cis) to induce kidney injury. Serum NGAL was measured as a marker of kidney injury.
Ravichandran et. al. AJP Renal 2017

Mouse Lipocalin-2/NGAL Quantikine ELISA Kit MLCN20
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 07/19/2017
Sample Tested: Serum

Almost as good as human kit.