Mouse LIX Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LIX in Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, recombinant rat CXCL1, 3/CINC-2 alpha, 3/CINC-2 beta, or recombinant porcine CXCL8 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 61905
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse LIX
    Val45-Ala118
    Accession # P50228
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse LIX (Catalog # 433-MC)
    under non-reducing conditions only
    • Mouse LIX Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse LIX Antibody (Catalog # MAB433)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse LIX Biotinylated Antibody (Catalog # BAF433)
  • ELISA Standard
     
    Recombinant Mouse LIX Protein (Catalog # 433-MC)
  • Neutralization
    Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse LIX.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody. Recombinant Mouse LIX (Catalog # 433-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse LIX (0.2 µg/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433). The ND50 is typically 0.5-2.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIX
LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemokinetic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus.  The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55.  Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132.  This may reduce the MW in SDS-PAGE by as much as 3 kDa.  The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity.  Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX.  Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.
  • Entrez Gene IDs:
    20311 (Mouse); 60665 (Rat)
  • Alternate Names:
    LIX
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing Enterocolitis
    Authors: Hongpeng Jia
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1beta-induced peritonitis.
    Authors: Song J, Wu C, Zhang X, Sorokin L
    J Immunol, 2013;190(1):401-10.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  3. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  4. Castration-induced changes in mouse epididymal white adipose tissue.
    Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
    Mol. Cell. Endocrinol., 2011;345(1):58-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005;175(4):2589-97.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004;72(12):7247-56.
    Species: Mouse
    Sample Type:
    Application: In vivo
  7. Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Fetal Pediatr Pathol, 2004;23(2):145-57.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
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