Mouse LRIG1 Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse LRIG1
    Ala37-Thr794
    Accession # P70193
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse LRIG1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109).  Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LRIG1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunocytochemistry
LRIG1 in bEnd.3 Mouse Cell Line. LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRIG1

LRIG-1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIG gene family.  It is widely expressed, and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons.  LRIG-1 is believed to negatively regulate the ErbB family of receptors.  In particular, and in a ligand-independent manner, LRIG-1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number.  Alternatively, LRIG-1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction.  Mature mouse LRIG-1 is a 1057 amino acid (aa) type I transmembrane protein (SwissProt #:P70193).  It contains a large 762 amino acid (aa) extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region.  The ECD contains 17 LRRs (aa’s 35-491) and three C2-type Ig-like domains (aa’s 497-781).  These two domain types are each sufficient for EGFR binding.  There one potential alternative splice form that a deletion of aa 875-923.  The LRIG-1 ECD undergoes proteolysis, generating 90-105 and 60-70 kDa soluble fragments.  Over aa 37-794, human LRIG-1 shares 97% and 90% aa sequence identity with rat and human LRIG-1, respectively.

  • Long Name:
    Leucine-rich Repeats and Immunoglobulin-like Domains 1
  • Entrez Gene IDs:
    26018 (Human); 16206 (Mouse); 312574 (Rat)
  • Alternate Names:
    D6Bwg0781e; Img; LIG1; LIG-1; LRIG1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss
    Authors: JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage
    Nat. Cell Biol., 2017;19(6):666-676.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing.
    Authors: Zhao, Huashan, Qiao, Jingqiao, Zhang, Shoubing, Zhang, Huishan, Lei, Xiaohua, Wang, Xinyue, Deng, Zhili, Ning, Lina, Cao, Yujing, Guo, Yong, Liu, Shuang, Duan, Enkui
    Sci Rep, 2015;5(0):7913.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Overexpression of epigen during embryonic development induces reversible, epidermal growth factor receptor-dependent sebaceous gland hyperplasia.
    Authors: Dahlhoff M, Frances D, Kloepper J, Paus R, Schafer M, Niemann C, Schneider M
    Mol Cell Biol, 2014;34(16):3086-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Kindlin-1 controls Wnt and TGF-beta availability to regulate cutaneous stem cell proliferation.
    Authors: Rognoni E, Widmaier M, Jakobson M, Ruppert R, Ussar S, Katsougkri D, Bottcher R, Lai-Cheong J, Rifkin D, McGrath J, Fassler R
    Nat Med, 2014;20(4):350-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling.
    Authors: Wong, Vivian W, Stange, Daniel E, Page, Mahalia, Buczacki, Simon, Wabik, Agnieszk, Itami, Satoshi, van de Wetering, Marc, Poulsom, Richard, Wright, Nicholas, Trotter, Matthew, Watt, Fiona M, Winton, Doug J, Clevers, Hans, Jensen, Kim B
    Nat Cell Biol, 2012;14(4):401-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Transcription factor Oct1 is a somatic and cancer stem cell determinant.
    Authors: Maddox, Jessica, Shakya, Arvind, South, Samuel, Shelton, Dawne, Andersen, Jared N, Chidester, Stephani, Kang, Jinsuk, Gligorich, Keith M, Jones, David A, Spangrude, Gerald J, Welm, Bryan E, Tantin, Dean
    PLoS Genet, 2012;8(11):e1003048.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Delayed cutaneous wound healing and aberrant expression of hair follicle stem cell markers in mice selectively lacking Ctip2 in epidermis.
    Authors: Liang X, Bhattacharya S, Bajaj G
    PLoS ONE, 2012;7(2):e29999.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation.
    Authors: Baker CM, Verstuyf A, Jensen KB
    Dev. Biol., 2010;343(1):40-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. TCF/Lef1 activity controls establishment of diverse stem and progenitor cell compartments in mouse epidermis.
    Authors: Petersson M, Brylka H, Kraus A
    EMBO J., 2010;30(15):3004-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis.
    Authors: Jensen KB, Driskell RR, Watt FM
    Nat Protoc, 2010;5(5):898-911.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 11 Citations
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