Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Lymphotoxin beta R/TNFRSF3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0 derived recombinant mouse Lymphotoxin beta Receptor
Ser28-Pro218
Accession # P50284

Specificity

Detects mouse Lymphotoxin beta R/TNFRSF3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

Detection of Mouse Lymphotoxin beta R/TNFRSF3 antibody by Western Blot.

Detection of Mouse Lymphotoxin beta R/TNFRSF3 by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10081) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lymphotoxin beta R/TNFRSF3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lymphotoxin beta R/TNFRSF3 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

Lymphotoxin beta R/TNFRSF3 in Mouse Liver.

Lymphotoxin beta R/TNFRSF3 was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10081) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces in hepatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

2 µg/mL
Sample: C2C12 mouse myoblast cell line, Hepa 1‑6 mouse hepatoma cell line, and Mouse stomach tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References

  1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  2. Rennert, P.D. et al. (1998) Immunity 9:71..
  3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756. .
  4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618. .
  5. Crowe, P.D. et al. (1994) Science 264:707.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P50284

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

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Product Specific Notices for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

For research use only

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Protocols

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