Mouse LYVE-1 Antibody

Name: Mouse LYVE-1 Antibody
Brand: R&D Systems
Rating: 5 (2 reviews)

(28 citations)

(2 Reviews)

Datasheet / CoA / SDS

Immunohistochemistry

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Western Blot

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Product Details

Citations (28)

FAQs

Supplemental Products

Reviews

Species Reactivity

Mouse
Specificity

Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human LYVE-1 is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.25 µg/mL

See below
Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry

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LYVE‑1 in Mouse Liver. LYVE‑1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot

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Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE‑1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively. LYVE-1 shares 41% homology with the other known HA receptor, CD44. The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican. Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes. Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature.

Long Name:

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Entrez Gene IDs:

10894 (Human); 114332 (Mouse); 293186 (Rat)

Alternate Names:

cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Species	Applications	Sample Type
VEGF-C promotes the development of lymphatics in bone and bone loss Authors: D Hominick, A Silva, N Khurana, Y Liu, PC Dechow, JQ Feng, B Pytowski, JM Rutkowski, K Alitalo, MT Dellinger Elife, 2018;7(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC-P Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response Authors: BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br Cell Mol Gastroenterol Hepatol, 2018;5(1):67-82.e1. Species: Mouse Sample Type: Whole Tissue Application: IHC-P Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases Authors: M García-Cab, J Paupert, S Blacher, M Van de Vel, AR Quesada, MA Medina, A Noël J Hematol Oncol, 2017;10(1):122. Species: Mouse Sample Type: Whole Tissue Application: IHC cIAP2 promotes gallbladder cancer invasion and lymphangiogenesis by activating the NF-?B pathway Authors: X Jiang, C Li, B Lin, H Hong, L Jiang, S Zhu, X Wang, N Tang, X Li, F She, Y Chen Cancer Sci., 2017;108(6):1144-1156. Species: Mouse Sample Type: Whole Tissue Application: IHC Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation Proc Natl Acad Sci USA, 2016;113(20):E2842-51. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: IP Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Authors: Boksik Cha Genes Dev, 2016;30(12):1454-69. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Dev Biol, 2016;409(1):218-33. Species: Mouse Sample Type: Whole Tissue Application: IHC-OCT embedded Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. Authors: Zhou Y, Williams J, Smallwood P, Nathans J PLoS ONE, 2015;10(12):e0143650. Species: Mouse Sample Type: Whole Tissue Application: IHC-Frozen A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research. Authors: Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M PLoS ONE, 2015;10(4):e0122976. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Lymph flow regulates collecting lymphatic vessel maturation in vivo. Authors: Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M J Clin Invest, 2015;125(8):2995-3007. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis. Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A PLoS ONE, 2014;9(9):e106976. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process. Authors: Kizhatil, Krishnak, Ryan, Margaret, Marchant, Jeffrey, Henrich, Stephen, John, Simon W PLoS Biol, 2014;12(7):e1001912. Species: Mouse Sample Type: Whole Tissue Application: IHC VEGF-C-dependent stimulation of lymphatic function ameliorates experimental inflammatory bowel disease. Authors: D'Alessio S, Correale C, Tacconi C, Gandelli A, Pietrogrande G, Vetrano S, Genua M, Arena V, Spinelli A, Peyrin-Biroulet L, Fiocchi C, Danese S J Clin Invest, 2014;124(9):3863-78. Species: Human Sample Type: Whole Tissue Application: IHC Lymphatic function is required prenatally for lung inflation at birth. Authors: Jakus Z, Gleghorn J, Enis D, Sen A, Chia S, Liu X, Rawnsley D, Yang Y, Hess P, Zou Z, Yang J, Guttentag S, Nelson C, Kahn M J Exp Med, 2014;211(5):815-26. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. Authors: Luckashenak N, Wahe A, Breit K, Brakebusch C, Brocker T J Immunol, 2013;190(1):27-35. Species: Mouse Sample Type: Whole Tissue Application: IHC Deletion of tetraspanin CD9 diminishes lymphangiogenesis in vivo and in vitro. Authors: Iwasaki T, Takeda Y, Maruyama K, Yokosaki Y, Tsujino K, Tetsumoto S, Kuhara H, Nakanishi K, Otani Y, Jin Y, Kohmo S, Hirata H, Takahashi R, Suzuki M, Inoue K, Nagatomo I, Goya S, Kijima T, Kumagai T, Tachibana I, Kawase I, Kumanogoh A J Biol Chem, 2013;288(4):2118-31. Species: Mouse Sample Type: Whole Cells Application: Cell selection Sphingosine 1-Phosphate-Induced Motility and Endocytosis of Dendritic Cells Is Regulated by SWAP-70 through RhoA. Authors: Ocana-Morgner C, Reichardt P, Chopin M, Braungart S, Wahren C, Gunzer M, Jessberger R J. Immunol., 2011;186(9):5345-55. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Angiopoietin-1 is essential in mouse vasculature during development and in response to injury. Authors: Jeansson M, Gawlik A, Anderson G J. Clin. Invest., 2011;121(6):2278-89. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen ALK1 signaling regulates early postnatal lymphatic vessel development. Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M Blood, 2010;115(8):1654-61. Species: Mouse Sample Type: Whole Tissue Application: IHC Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A J. Cell Biol., 2010;188(1):115-30. Species: Mouse Sample Type: Whole Cells Application: ICC Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth. Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42. Species: Mouse Sample Type: Whole Cells Application: ICC Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes. Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T EMBO J., 2009;28(9):1319-31. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A J. Immunol., 2008;181(9):6189-200. Species: Mouse Sample Type: Whole Cells Application: ICC Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice. Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA Biol. Reprod., 2008;79(3):450-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Thymic stromal lymphopoietin transgenic mice develop cryoglobulinemia and hepatitis with similarities to human hepatitis C liver disease. Authors: Kowalewska J, Muhlfeld AS, Hudkins KL, Yeh MM, Farr AG, Ravetch JV, Alpers CE Am. J. Pathol., 2007;170(3):981-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy. Authors: Bagley RG, Weber W, Rouleau C, Teicher BA Cancer Res., 2005;65(21):9741-50. Species: Mouse Sample Type: Whole Tissue Application: IHC Expand to show all Citations

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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

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Mouse LYVE-1 Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Basic

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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