Mouse LYVE-1 Antibody

Catalog # Availability Size / Price Qty
AF2125
AF2125-SP
Mouse LYVE-1 Antibody in Data
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Product Details
Citations (43)
FAQs
Supplemental Products
Reviews (3)

Mouse LYVE-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human LYVE-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry LYVE‑1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

LYVE‑1 in Mouse Liver. LYVE-1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse LYVE-1 antibody by Western Blot. View Larger

Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE-1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively. LYVE-1 shares 41% homology with the other known HA receptor, CD44. The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican. Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes. Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature.

Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

Product Datasheets

Citations for Mouse LYVE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation
    Authors: CJ Park, PC Lin, S Zhou, R Barakat, ST Bashir, JM Choi, JA Cacioppo, OR Oakley, DM Duffy, JP Lydon, CJ Ko
    Cell Rep, 2020;31(2):107496.
    Species: Mouse
    Sample Types: Granulosa Cells
    Applications: IHC
  2. Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D
    Authors: A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski
    Front Physiol, 2020;11(0):363.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours
    Authors: E Song, T Mao, H Dong, LSB Boisserand, S Antila, M Bosenberg, K Alitalo, JL Thomas, A Iwasaki
    Nature, 2020;577(7792):689-694.
    Species: Mouse
    Sample Types: Tissue
    Applications: IF
  4. Compartmentalized gut lymph node drainage dictates adaptive immune responses
    Authors: D Esterházy, MCC Canesso, L Mesin, PA Muller, TBR de Castro, A Lockhart, M ElJalby, AMC Faria, D Mucida
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Lymphatic impairment leads to pulmonary tertiary lymphoid organ formation and alveolar damage
    Authors: HO Reed, L Wang, J Sonett, M Chen, J Yang, L Li, P Aradi, Z Jakus, J D'Armiento, WW Hancock, ML Kahn
    J. Clin. Invest., 2019;129(6):2514-2526.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Receptor?interacting serine/threonine?protein kinase 1 promotes the progress and lymph metastasis of gallbladder cancer
    Authors: G Zhu, Q Du, X Chen, X Wang, N Tang, F She, Y Chen
    Oncol. Rep., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth
    Authors: SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar
    PLoS ONE, 2019;14(7):e0220341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Associating liver partition and portal vein ligation for staged hepatectomy: establishment of an animal model with insufficient liver remnant
    Authors: A Dili, V Lebrun, C Bertrand, IA Leclercq
    Lab. Invest., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. VEGF-C promotes the development of lymphatics in bone and bone loss
    Authors: D Hominick, A Silva, N Khurana, Y Liu, PC Dechow, JQ Feng, B Pytowski, JM Rutkowski, K Alitalo, MT Dellinger
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Lymphatic Vascular Structures: A New Aspect in Proliferative Diabetic Retinopathy
    Authors: E Gucciardo, S Loukovaara, P Salven, K Lehti
    Int J Mol Sci, 2018;19(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation
    Authors: M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet
    Stem Cell Reports, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. uPARAP/Endo180 receptor is a gatekeeper of VEGFR-2/VEGFR-3 heterodimerisation during pathological lymphangiogenesis
    Authors: T Durré, F Morfoisse, C Erpicum, M Ebroin, S Blacher, M García-Cab, C Deroanne, T Louis, C Balsat, M Van de Vel, S Kaijalaine, F Kridelka, L Engelholm, I Struman, K Alitalo, N Behrendt, J Paupert, A Noel
    Nat Commun, 2018;9(1):5178.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response
    Authors: BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br
    Cell Mol Gastroenterol Hepatol, 2018;5(1):67-82.e1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204
    Authors: LKM Lam, AM Watson, KD Ryman, WB Klimstra
    Npj Vaccines, 2018;3(0):5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema
    Authors: GD García Nor, CL Ly, DA Cuzzone, RP Kataru, GE Hespe, JS Torrisi, JJ Huang, JC Gardenier, IL Savetsky, MD Nitti, JZ Yu, S Rehal, BJ Mehrara
    Nat Commun, 2018;9(1):1970.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Mir-126 is a conserved modulator of lymphatic development
    Authors: Z Kontarakis, A Rossi, S Ramas, MT Dellinger, DYR Stainier
    Dev. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: Western Blot
  17. RIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway
    Authors: CZ Li, XJ Jiang, B Lin, HJ Hong, SY Zhu, L Jiang, XQ Wang, NH Tang, FF She, YL Chen
    Onco Targets Ther, 2018;11(0):2875-2890.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. cIAP2 promotes gallbladder cancer invasion and lymphangiogenesis by activating the NF-?B pathway
    Authors: X Jiang, C Li, B Lin, H Hong, L Jiang, S Zhu, X Wang, N Tang, X Li, F She, Y Chen
    Cancer Sci., 2017;108(6):1144-1156.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases
    Authors: M García-Cab, J Paupert, S Blacher, M Van de Vel, AR Quesada, MA Medina, A Noël
    J Hematol Oncol, 2017;10(1):122.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation
    Proc Natl Acad Sci USA, 2016;113(20):E2842-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  23. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
  25. Lymph flow regulates collecting lymphatic vessel maturation in vivo.
    Authors: Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M
    J Clin Invest, 2015;125(8):2995-3007.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
    Authors: Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M
    PLoS ONE, 2015;10(4):e0122976.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015;10(12):e0143650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. VEGF-C-dependent stimulation of lymphatic function ameliorates experimental inflammatory bowel disease.
    Authors: D'Alessio S, Correale C, Tacconi C, Gandelli A, Pietrogrande G, Vetrano S, Genua M, Arena V, Spinelli A, Peyrin-Biroulet L, Fiocchi C, Danese S
    J Clin Invest, 2014;124(9):3863-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process.
    Authors: Kizhatil, Krishnak, Ryan, Margaret, Marchant, Jeffrey, Henrich, Stephen, John, Simon W
    PLoS Biol, 2014;12(7):e1001912.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
    Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A
    PLoS ONE, 2014;9(9):e106976.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Lymphatic function is required prenatally for lung inflation at birth.
    Authors: Jakus Z, Gleghorn J, Enis D, Sen A, Chia S, Liu X, Rawnsley D, Yang Y, Hess P, Zou Z, Yang J, Guttentag S, Nelson C, Kahn M
    J Exp Med, 2014;211(5):815-26.
  32. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo.
    Authors: Luckashenak N, Wahe A, Breit K, Brakebusch C, Brocker T
    J Immunol, 2013;190(1):27-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Deletion of tetraspanin CD9 diminishes lymphangiogenesis in vivo and in vitro.
    Authors: Iwasaki T, Takeda Y, Maruyama K, Yokosaki Y, Tsujino K, Tetsumoto S, Kuhara H, Nakanishi K, Otani Y, Jin Y, Kohmo S, Hirata H, Takahashi R, Suzuki M, Inoue K, Nagatomo I, Goya S, Kijima T, Kumagai T, Tachibana I, Kawase I, Kumanogoh A
    J Biol Chem, 2013;288(4):2118-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  34. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury.
    Authors: Jeansson M, Gawlik A, Anderson G
    J. Clin. Invest., 2011;121(6):2278-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. Sphingosine 1-Phosphate-Induced Motility and Endocytosis of Dendritic Cells Is Regulated by SWAP-70 through RhoA.
    Authors: Ocana-Morgner C, Reichardt P, Chopin M, Braungart S, Wahren C, Gunzer M, Jessberger R
    J. Immunol., 2011;186(9):5345-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2010;115(8):1654-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
    Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A
    J. Cell Biol., 2010;188(1):115-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
    Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
    EMBO J., 2009;28(9):1319-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  39. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
    Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  40. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008;181(9):6189-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  41. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008;79(3):450-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  42. Thymic stromal lymphopoietin transgenic mice develop cryoglobulinemia and hepatitis with similarities to human hepatitis C liver disease.
    Authors: Kowalewska J, Muhlfeld AS, Hudkins KL, Yeh MM, Farr AG, Ravetch JV, Alpers CE
    Am. J. Pathol., 2007;170(3):981-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy.
    Authors: Bagley RG, Weber W, Rouleau C, Teicher BA
    Cancer Res., 2005;65(21):9741-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse LYVE-1 Antibody

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Mouse LYVE-1 Antibody
By Anonymous on 01/08/2021
Application: ICC/IF Sample Tested: Heart tissue Species: Mouse

Mouse LYVE-1 Antibody
By Anonymous on 02/02/2017
Application: IHC Sample Tested: Lymph node tissue Species: Mouse

In paraffin embedded sample.
Antigen retrieval with Tris-EDTA Buffer pH 9.0 and fixation with Methanol.
Blocking with TBS 2% BSA -0.5% Triton X-100.
Incubation of Lyve1 (AF2125) at a dilution of 1:400 (O/N at 4C).


Mouse LYVE-1 Antibody
By Anonymous on 09/07/2016
Application: Whole mount immunofluorescence Sample Tested: Whole-mount ear dermis Species: Mouse

Mouse ear dermis was fixed in 2%PFA and blocked/permeabilized overnight in TBS/5% donkey serum/0.5% Tx100 before incubation in AF2125 at a dilution of 1:250 (O/N at 4C). Antibody was then detected using an alexafluor 488 labeled donkey anti goat secondary.