Mouse LYVE-1 Antibody

(26 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LYVE-1 in direct ELISAs and Western blots. In these formats, approximately 50% cross-reactivity with recombinant human LYVE-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1 (R&D Systems, Catalog # 2125-LY)
    Ala24-Thr234
    Accession # Q8BHC0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
LYVE‑1 in Mouse Liver. LYVE‑1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial  cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE‑1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively. LYVE-1 shares 41% homology with the other known HA receptor, CD44. The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican. Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes. Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature.

  • Long Name:
    Lymphatic Vessel Endothelial Hyaluronan Receptor 1
  • Entrez Gene IDs:
    10894 (Human); 114332 (Mouse); 293186 (Rat)
  • Alternate Names:
    cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases
    Authors: M García-Cab, J Paupert, S Blacher, M Van de Vel, AR Quesada, MA Medina, A Noël
    J Hematol Oncol, 2017;10(1):122.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. cIAP2 promotes gallbladder cancer invasion and lymphangiogenesis by activating the NF-?B pathway
    Authors: X Jiang, C Li, B Lin, H Hong, L Jiang, S Zhu, X Wang, N Tang, X Li, F She, Y Chen
    Cancer Sci., 2017;108(6):1144-1156.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation
    Proc Natl Acad Sci USA, 2016;113(20):E2842-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  5. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IP
  6. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
  7. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  8. Lymph flow regulates collecting lymphatic vessel maturation in vivo.
    Authors: Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M
    J Clin Invest, 2015;125(8):2995-3007.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
    Authors: Bianchi R, Teijeira A, Proulx S, Christiansen A, Seidel C, Rulicke T, Makinen T, Hagerling R, Halin C, Detmar M
    PLoS ONE, 2015;10(4):e0122976.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  10. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015;10(12):e0143650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Frozen
  11. Lymphatic function is required prenatally for lung inflation at birth.
    Authors: Jakus Z, Gleghorn J, Enis D, Sen A, Chia S, Liu X, Rawnsley D, Yang Y, Hess P, Zou Z, Yang J, Guttentag S, Nelson C, Kahn M
    J Exp Med, 2014;211(5):815-26.
  12. VEGF-C-dependent stimulation of lymphatic function ameliorates experimental inflammatory bowel disease.
    Authors: D'Alessio S, Correale C, Tacconi C, Gandelli A, Pietrogrande G, Vetrano S, Genua M, Arena V, Spinelli A, Peyrin-Biroulet L, Fiocchi C, Danese S
    J Clin Invest, 2014;124(9):3863-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  13. Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process.
    Authors: Kizhatil, Krishnak, Ryan, Margaret, Marchant, Jeffrey, Henrich, Stephen, John, Simon W
    PLoS Biol, 2014;12(7):e1001912.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  14. Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
    Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A
    PLoS ONE, 2014;9(9):e106976.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  15. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo.
    Authors: Luckashenak N, Wahe A, Breit K, Brakebusch C, Brocker T
    J Immunol, 2013;190(1):27-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  16. Deletion of tetraspanin CD9 diminishes lymphangiogenesis in vivo and in vitro.
    Authors: Iwasaki T, Takeda Y, Maruyama K, Yokosaki Y, Tsujino K, Tetsumoto S, Kuhara H, Nakanishi K, Otani Y, Jin Y, Kohmo S, Hirata H, Takahashi R, Suzuki M, Inoue K, Nagatomo I, Goya S, Kijima T, Kumagai T, Tachibana I, Kawase I, Kumanogoh A
    J Biol Chem, 2013;288(4):2118-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Cell selection
  17. Sphingosine 1-Phosphate-Induced Motility and Endocytosis of Dendritic Cells Is Regulated by SWAP-70 through RhoA.
    Authors: Ocana-Morgner C, Reichardt P, Chopin M, Braungart S, Wahren C, Gunzer M, Jessberger R
    J. Immunol., 2011;186(9):5345-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  18. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury.
    Authors: Jeansson M, Gawlik A, Anderson G
    J. Clin. Invest., 2011;121(6):2278-89.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  19. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2010;115(8):1654-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  20. Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
    Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A
    J. Cell Biol., 2010;188(1):115-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  21. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
    Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  22. Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
    Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
    EMBO J., 2009;28(9):1319-31.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  23. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008;181(9):6189-200.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  24. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008;79(3):450-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Thymic stromal lymphopoietin transgenic mice develop cryoglobulinemia and hepatitis with similarities to human hepatitis C liver disease.
    Authors: Kowalewska J, Muhlfeld AS, Hudkins KL, Yeh MM, Farr AG, Ravetch JV, Alpers CE
    Am. J. Pathol., 2007;170(3):981-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  26. Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy.
    Authors: Bagley RG, Weber W, Rouleau C, Teicher BA
    Cancer Res., 2005;65(21):9741-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 26 Citations
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

CTS014
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NL996
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Whole mount immunofluorescence .

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse LYVE-1 Antibody AF2125
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Excellent
 IHC Mouse Anonymous 02/02/2017
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Image Details
Immunohistochemistry Mouse LYVE-1 Antibody AF2125
Immunohistochemistry: Mouse LYVE-1 Antibody [AF2125]

Summary

ApplicationImmunohistochemistry
Sample TestedLymph node tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsIn paraffin embedded sample.Antigen retrieval with Tris-EDTA Buffer pH 9.0 and fixation with Methanol.Blocking with TBS 2% BSA -0.5% Triton X-100.Incubation of Lyve1 (AF2125) at a dilution of 1:400 (O/N at 4C).
Whole mount immunofluorescence  Mouse LYVE-1 Antibody AF2125
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Excellent
 Whole mount immunofluorescence Mouse Anonymous 09/07/2016
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Whole mount immunofluorescence  Mouse LYVE-1 Antibody AF2125
Whole mount immunofluorescence : Mouse LYVE-1 Antibody [AF2125]

Summary

ApplicationWhole mount immunofluorescence
Sample TestedWhole-mount ear dermis
SpeciesMouse

Other Experimental Details

Other Experimental DetailsMouse ear dermis was fixed in 2%PFA and blocked/permeabilized overnight in TBS/5% donkey serum/0.5% Tx100 before incubation in AF2125 at a dilution of 1:250 (O/N at 4C). Antibody was then detected using an alexafluor 488 labeled donkey anti goat secondary.

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