Mouse LYVE-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2125
Product Details
Citations (3)
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Mouse LYVE-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LYVE-1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) LYVE-1 and less than 1% cross-reactivity with rhCD44 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LYVE‑1 (Catalog # 2125-LY)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver and spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

References
  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.
  7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
  8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

Product Datasheets

Citations for Mouse LYVE-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3
    Authors: WS Chen, Z Cao, S Sugaya, MJ Lopez, VG Sendra, N Laver, H Leffler, UJ Nilsson, J Fu, J Song, L Xia, P Hamrah, N Panjwani
    Nat Commun, 2016;7(0):11302.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice.
    Authors: Fu J, Gerhardt H, McDaniel JM, Xia B, Liu X, Ivanciu L, Ny A, Hermans K, Silasi-Mansat R, McGee S, Nye E, Ju T, Ramirez MI, Carmeliet P, Cummings RD, Lupu F, Xia L
    J. Clin. Invest., 2008;118(11):3725-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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