Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 800516
View all M-Cadherin/Cadherin-15 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse M-Cadherin/Cadherin-15
Val22-Ala605
Accession # P33146

Specificity

Detects mouse M-Cadherin/Cadherin-15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human M-Cadherin, recombinant mouse (rm) Cadherin-13, or rmP-Cadherin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse M‑Cadherin/Cadherin‑15 Antibody

M-Cadherin/Cadherin-15 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

M‑Cadherin/Cadherin‑15 in C2C12 Mouse Cell Line.

M-Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cells differentiated for 5 days with 5% equine serum using Rat Anti-Mouse M-Cadherin/Cadherin-15 Monoclonal Antibody (Catalog # MAB7677) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of developing myotubes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse M‑Cadherin/Cadherin‑15 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line differentiated for 5 days with 5% equine serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-Cadherin/Cadherin-15

CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.

Long Name

M-Cadherin [Myotubule]

Alternate Names

Cadherin-15, CDH14, CDH15, CDHM, MCAD, MCadherin

Entrez Gene IDs

1013 (Human); 12555 (Mouse); 361432 (Rat)

Gene Symbol

CDH15

UniProt

P33146

Additional M-Cadherin/Cadherin-15 Products

Product Documents for Mouse M‑Cadherin/Cadherin‑15 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse M‑Cadherin/Cadherin‑15 Antibody

For research use only

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