Mouse Magnetic Luminex Screening Assay (LXSAMSM): R&D Systems

Mouse Magnetic Luminex Assay

(5 citations)
(1 Review)
  
  • Assay Type
    Bead-based multiplex assay for the Luminex® platform
  • Format
    1 x 96-well microplate and magnetic antibody-coated beads
  • Analytes Detected
    Please see table below.
  • Performance Validation
     
    Product Features

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Features
  • User defines up to 50 analytes of interest
  • Wide selection of analytes
  • Requires a small sample volume (50 µL)
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
  • Quick turn-around time: Kits are typically shipped within 3-5 days of ordering.*

*Please allow additional time when placing orders through a distributor.

 

Kit Contents
  • Premixed antibody-coated magnetic bead cocktail
  • Premixed biotinylated detection antibody cocktail
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One Flat-bottom 96-well Microplate
  • Foil Plate Sealers
  • Mixing Bottle
  • Certificate of Analysis

 

Available Luminex Assays Analytes

The following chart indicates the bead region, sensitivity, high standard value, and recommended samples and their starting dilution for each analyte.

Analyte Sensitivity
(MDD)pg/mL
Standard
Curve Range (pg/mL)
Starting Sample Dilution Magnetic
Bead Region
Cell Culture
Supernates/ Lysates
Serum/
Plasma
Adiponectin 7.55 18.6-4,520 1:2 1:4,000 34
Angiopoietin-2 30.8 638-155,080 1:2 1:2 67
BAFF/BLyS 11.1 56.2-13,660 1:2 1:2 57
ß-NGF 1.06 12.3-3,000 1:2 1:2 43
C1q R1/CD93 5.33 336-81,680 1:2 1:2 64
CCL2/JE/MCP-1 134 221-53,720 1:2 1:2 18
CCL3/MIP-1 alpha 0.452 0.905-220 1:2 1:2 46
CCL4/MIP-1 beta 77.4 169-41,160 1:2 1:2 51
CCL5/RANTES 19.1 88.9-21,610 1:2 1:2 38
CCL7/MARC 1.69 7.86-1,910 1:2 1:2 39
CCL8/MCP-2 0.283 8.15-1,980 1:2 1:200 38
CCL11/Eotaxin 1.46 18.6-4,530 1:2 1:2 74
CCL12/MCP-5 0.613 8.23-2,000 1:2 1:2 42
CCL19/MIP-3 beta 2.28 9.88-2,400 1:2 1:2 19
CCL20/MIP-3 alpha 39.5 117-28,430 1:2 1:2 48
CCL21/6Ckine 0.061 2.51-610 1:2 1:50 72
CCL22/MDC 0.965 28.4-6,910 1:2 1:2 75
Chitinase 3-like 1/YKL-40 18.2 77.0-18,700 1:2 1:50 20
CRP 13.3 67.5-16,400 1:2 1:200

63

CXCL1/KC 32.9 28.5-6,920 1:2 1:2 13
CXCL2/MIP-2 1.97 4.4-1,080 1:2 1:2 20
CXCL10/IP-10/ CRG-2 6.85 48-11,660 1:2 1:2 37
CXCL12/SDF-1 alpha 19.1 280-67,930 1:2 1:2 54
CXCL13/BLC/BCA-1 19.3 187-45,530 1:2 1:2 21
CXCL16 1.47 9.01-2,190 1:2 1:2 22
Cystatin C 2.61 60.8-14,780 1:2 1:200 30
DPPIV/CD26 135 394-95,790 1:2 1:2 25
Dkk-1 31.8 365-88,720 1:2 1:2 77
EGF 0.089 0.535-130 1:2 1:2 26
EGF R 25.4 683-165,910 1:2 1:200 22

EMMPRIN

10.5 136-32,990 1:2 1:2 19
Endoglin 6.15 124-30,150 1:2 1:2 33
Factor D/Adipsin 390 4,730-1,149,300 1:2 1:200 75
FABP4/A-FABP 5.40 34.1-8,290 1:2 1:50 13
Fas Ligand 2.34 32.7-7,940 1:2 1:2 27
FGF-basic 41.3 48.1-11,700 1:2 1:2 65
FGF-21 0.914 24.0-5,820 1:2 1:2 61
Galectin-3 0.255 3.34-812 1:2 1:200 28
G-CSF 0.774 14.7-3,580 1:2 1:2 39
GDF-15 0.630 14.7-3,570 1:2 1:2 15
Glucagon 58.5 136-32,940 1:2 1:2 34
GM-CSF 1.64 11.6-2,830 1:2 1:2 12
Granzyme B 0.989 20.0-4,850 1:2 1:2 63
Haptoglobin 118 2,131-517,720 1:2 1:4,000 30
HGF 3.33 39.6-9,620 1:2 1:2

12

ICAM-1 52.7 627-152,270 1:2 1:2 35
IFN-gamma 1.85 12.3-2,990 1:2 1:2 33
IGF-I 3.46 46.0-11,180 1:2 1:50 55
IGFBP-1 0.218 1.85-450 1:2 1:200 33
IGFBP-3 7.46 53.1-12,900 1:2 1:4,000 65
IL-1 alpha 8.17 28.6-6,960 1:2 1:2 47
IL-1 beta 41.8 221-53,780 1:2 1:2 19
IL-2 0.481 7.7-1,860 1:2 1:2 22
IL-3 0.200 2.63-640 1:2 1:2 34
IL-4 53.0 56.4-13,700 1:2 1:2 25
IL-5 0.237 4.7-1,130 1:2 1:2 26
IL-6 2.30 24.6-5,970 1:2 1:2 27
IL-6 R alpha 26.8 431-104,770 1:2 1:2 18
IL-7 35.4 267-64,770 1:2 1:2 14
IL-10 8.20 11.7-2,840 1:2 1:2 28
IL-12 p70 12.8 42.1-10,240 1:2 1:2 15
IL-13 6.46 59.2-14,390 1:2 1:2 29
IL-16 0.547 14.2-3,460 1:2 1:2 35
IL-17A 7.08 56.2-13,660 1:2 1:2 30
IL-17E/IL-25 8.88 147-35,790 1:2 1:2 55
IL-23 p19 47.7 108-26,150 1:2 1:2 42
IL-27 1.84 45.2-10,990 1:2 1:2 56
IL-33 57.1 79.1-19,220 1:2 1:2 43
LDL R 411 1,227-298,080 1:2 1:2 37
Lipocalin-2/NGAL 66.1 373-90,620 1:2 1:50 44
LIX 2.02 18.1-4,410 1:2 1:2 66
M-CSF 0.404 1.85-450 1:2 1:2 45
MMP-2 48.8 214-51,970 1:2 1:200 37
MMP-3 0.332 15.3-3,720 1:2 1:200 62
MMP-8 2,109 4,657-1,131,617 1:2 1:2 61
MMP-9 47.1 92.8-22,560 1:2 1:50 62
MMP-12 0.424 20.9-5,090 1:2 1:2 73
Nephrin 154 680-165,220 1:2 1:2 38
Oncostatin M 6.26 72.3-17,580 1:2 1:2 64
Osteopontin 2.37 36.4-8,840 1:2 1:50 56
Osteoprotegerin 6.32 48.9-11,890 1:2 1:2 39
Pancreatic Polypeptide 23.3 252-61,160 1:2 1:2 45
PDGF-AA 0.903 4.16-1,010 1:2 1:200/1:2 14
PDGF-AB 0.725 8.20-1,993 1:2 1:2 46
PDGF-BB 1.49 29.4-7,150 1:2 1:50 27
Periostin/OSF-2 201 1,370-332,890 1:2 1:200 67
PlGF-2 1.70 9.37-2,270 1:2 1:2 44
Podocalyxin 5.03 115-28,000 1:2 1:2 77
P-Selectin 2.60 138-33,580 1:2 1:2 46
Prolactin 2.67 64.0-15,550 1:2 1:2 44
Proprotein Convertase 9/ PCSK9 289 430-104,500 1:2 1:2 53
RAGE 18.0 54.3-13,200 1:2 1:2 78
Renin 13.2 129-31,300 1:2 1:2 47
Resistin 0.737 3.3-800 1:2 1:50 36
S100A8 61.3 278-67,490 1:2 1:2 20
S100A9 3.55 110-26,720 1:2 1:2 21
Serpin E1/PAI-1 5.71 30.2-7,340 1:2 1:50 43
SOST 2.27 16.3-3,950 1:2 1:2 48
SP-D 11.0 234-56,760 1:2 1:2 12
Syndecan-1/CD138 288 1,066-259,100 1:2 1:2 51
Thrombospondin-4 17.5 256-62,290 1:2 1:200 13
TIM-1/KIM-1 13.1 257-62,380 1:2 1:2 72
TIMP-1 12.4 59.1-14,350 1:2 1:2 45
TIMP-4 14.4 123-29,850 1:2 1:2 47
TNF-alpha 1.47 3.7-900 1:2 1:2 14
TNF RI 0.124 7.86-1,910 1:2 1:2 15
TNF RII 2.48 46.7-11,340 1:2 1:2 74
TRANCE/RANK L 7.51 59.1-14,370 1:2 1:2 25
TWEAK 0.184 2.88-700 1:2 1:2 53
uPAR 88.2 1,080-262,520 1:2 1:2 76
VEGF 3.96 4.7-1,130 1:2 1:2 21
VEGF R2/KDR 27.5 267-64,810 1:2 1:2 26

*A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

 

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

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Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Species
Sample Type
  1. Nrf2 Modulates Host Defense during Streptococcus pneumoniae Pneumonia in Mice
    Authors: JC Gomez, H Dang, JR Martin, CM Doerschuk
    J. Immunol, 2016;197(7):2864-79.
    Species: Mouse
    Sample Type: BALF
  2. Positive Prehabilitative Effect of Intense Treadmill Exercise for Ameliorating Cancer Cachexia Symptoms in a Mouse Model
    J Cancer, 2016;7(15):2378-2387.
    Species: Mouse
    Sample Type: Plasma
  3. CF Lung Infection and Inflammation: IL-17 Pathophysiology and Therapeutic Intervention
    Authors: Daniel Hsu
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  4. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas
    Int J Cancer, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Lysate
  5. Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
    Nat Commun, 2016;7(0):12304.
    Species: Mouse
    Sample Type: Serum
Average Rating: 5 (Based on 1 review)

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: Mouse Magnetic Luminex Assay [LXSAMSM-10]

Summary

Sample TestedLung single-cell suspension

FAQs

  1. What is the difference between the Luminex® Screening Assay and the Luminex® Performance Assay?

    • In general, the sensitivity of Luminex® Screening Assays and Luminex® Performance Assays is similar. A Performance Assay consists of select analytes which have been thoroughly tested and validated together as a panel. The diluents provided in the performance kits and assay conditions have been optimized for best results with the analytes on the same panel. The Performance Assay is the most precise, reproducible and accurate Luminex tool offered by R&D Systems.

      The Screening Assay option is a flexible tool, providing the uncommon opportunity to customize by mixing and matching analytes across different panels. Although not as extensively tested as the Performance Assays, analytes on a Screening Assay are tested together when built and a final quality control test on each Screening Assay is performed prior to release. Since each Screening Assay is unique and built-to-order, additional days are required for order fulfillment and it is not characterized by the same level of optimization and validation testing as with the Performance assays.  

      For low abundance cytokines, High Sensitivity panels are available for Performance Assay, which are not available for Screening Assay. 

      The quality of the R&D Systems® Screening Assays is similar to the Luminex® Assays offered by other vendors. The following chart compares the different types of Luminex® Assays R&D Systems offers and may serve as a general guide for choosing which assay format is suitable to your experimental goals. 

      //www.rndsystems.com/products/which-luminex-assay-right-me

       

View all Luminex Assay FAQs

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LXSAMSM
1 Kit
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