Mouse Magnetic Luminex Assay

  (20 citations)
(4 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (20)
FAQs
Reviews
  • Assay Type
    Bead-based multiplex assay for the Luminex® platform
  • Format
    1 x 96-well microplate and magnetic antibody-coated beads
  • Analytes Detected
    Please see table below.
  • Performance Validation
     
    Product Features
 
Product Features

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Features
  • User defines up to 50 analytes of interest
  • Wide selection of analytes
  • Requires a small sample volume (50 µL)
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
  • Quick turn-around time: Kits are typically shipped within 3-5 days of ordering.*

*Please allow additional time when placing orders through a distributor.

 

Kit Contents
  • Premixed antibody-coated magnetic bead cocktail
  • Premixed biotinylated detection antibody cocktail
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One Flat-bottom 96-well Microplate
  • Foil Plate Sealers
  • Mixing Bottle
  • Certificate of Analysis

 

Available Luminex Assays Analytes

The following chart indicates the bead region, sensitivity, high standard value, and recommended samples and their starting dilution for each analyte.

Analyte Sensitivity
(MDD)pg/mL
Standard
Curve Range (pg/mL)
Starting Sample Dilution Magnetic
Bead Region
Cell Culture
Supernates/ Lysates
Serum/
Plasma
Adiponectin 7.55 18.6-4,520 1:2 1:4,000 34
Angiopoietin-2 30.8 638-155,080 1:2 1:2 67
BAFF/BLyS 11.1 56.2-13,660 1:2 1:2 57
ß-NGF 1.06 12.3-3,000 1:2 1:2 43
C1q R1/CD93 5.33 336-81,680 1:2 1:2 64
CCL2/JE/MCP-1 134 221-53,720 1:2 1:2 18
CCL3/MIP-1 alpha 0.452 0.905-220 1:2 1:2 46
CCL4/MIP-1 beta 77.4 169-41,160 1:2 1:2 51
CCL5/RANTES 19.1 88.9-21,610 1:2 1:2 38
CCL7/MARC 1.69 7.86-1,910 1:2 1:2 39
CCL8/MCP-2 0.283 8.15-1,980 1:2 1:200 38
CCL11/Eotaxin 1.46 18.6-4,530 1:2 1:2 74
CCL12/MCP-5 0.613 8.23-2,000 1:2 1:2 42
CCL19/MIP-3 beta 2.28 9.88-2,400 1:2 1:2 19
CCL20/MIP-3 alpha 39.5 117-28,430 1:2 1:2 48
CCL21/6Ckine 0.061 2.51-610 1:2 1:50 72
CCL22/MDC 0.965 28.4-6,910 1:2 1:2 75
Chitinase 3-like 1/YKL-40 18.2 77.0-18,700 1:2 1:50 20
CRP 13.3 67.5-16,400 1:2 1:200

63

CXCL1/KC 32.9 28.5-6,920 1:2 1:2 13
CXCL2/MIP-2 1.97 4.4-1,080 1:2 1:2 20
CXCL10/IP-10/ CRG-2 6.85 48-11,660 1:2 1:2 37
CXCL12/SDF-1 alpha 19.1 280-67,930 1:2 1:2 54
CXCL13/BLC/BCA-1 19.3 187-45,530 1:2 1:2 21
CXCL16 1.47 9.01-2,190 1:2 1:2 22
Cystatin C 2.61 60.8-14,780 1:2 1:200 30
DPPIV/CD26 135 394-95,790 1:2 1:2 25
Dkk-1 31.8 365-88,720 1:2 1:2 77
EGF 0.089 0.535-130 1:2 1:2 26
EGF R 25.4 683-165,910 1:2 1:200 22

EMMPRIN

10.5 136-32,990 1:2 1:2 19
Endoglin 6.15 124-30,150 1:2 1:2 33
Factor D/Adipsin 390 4,730-1,149,300 1:2 1:200 75
FABP4/A-FABP 5.40 34.1-8,290 1:2 1:50 13
Fas Ligand 2.34 32.7-7,940 1:2 1:2 27
FGF-basic 41.3 48.1-11,700 1:2 1:2 65
FGF-21 0.914 24.0-5,820 1:2 1:2 61
G-CSF 0.774 14.7-3,580 1:2 1:2 39
GDF-15 0.630 14.7-3,570 1:2 1:2 15
Glucagon 58.5 136-32,940 1:2 1:2 34
GM-CSF 1.64 11.6-2,830 1:2 1:2 12
Granzyme B 0.989 20.0-4,850 1:2 1:2 63
Haptoglobin 118 2,131-517,720 1:2 1:4,000 30
HGF 3.33 39.6-9,620 1:2 1:2

12

ICAM-1 52.7 627-152,270 1:2 1:2 35
IFN-gamma 1.85 12.3-2,990 1:2 1:2 33
IGF-I 3.46 46.0-11,180 1:2 1:50 55
IGFBP-1 0.218 1.85-450 1:2 1:200 33
IGFBP-3 7.46 53.1-12,900 1:2 1:4,000 65
IL-1 alpha 8.17 28.6-6,960 1:2 1:2 47
IL-1 beta 41.8 221-53,780 1:2 1:2 19
IL-2 0.481 7.7-1,860 1:2 1:2 22
IL-3 0.200 2.63-640 1:2 1:2 34
IL-4 53.0 56.4-13,700 1:2 1:2 25
IL-5 0.237 4.7-1,130 1:2 1:2 26
IL-6 2.30 24.6-5,970 1:2 1:2 27
IL-6 R alpha 26.8 431-104,770 1:2 1:2 18
IL-7 35.4 267-64,770 1:2 1:2 14
IL-10 8.20 11.7-2,840 1:2 1:2 28
IL-12 p70 12.8 42.1-10,240 1:2 1:2 15
IL-13 6.46 59.2-14,390 1:2 1:2 29
IL-16 0.547 14.2-3,460 1:2 1:2 35
IL-17A 7.08 56.2-13,660 1:2 1:2 30
IL-17E/IL-25 8.88 147-35,790 1:2 1:2 55
IL-23 p19 47.7 108-26,150 1:2 1:2 42
IL-27 1.84 45.2-10,990 1:2 1:2 56
IL-33 57.1 79.1-19,220 1:2 1:2 43
LDL R 411 1,227-298,080 1:2 1:2 37
Leptin 12.6 53-12,870 1:2 1:2 35
LIX 2.02 18.1-4,410 1:2 1:2 66
M-CSF 0.404 1.85-450 1:2 1:2 45
MMP-2 48.8 214-51,970 1:2 1:200 37
MMP-3 0.332 15.3-3,720 1:2 1:200 62
MMP-8 2,109 4,657-1,131,617 1:2 1:2 61
MMP-9 47.1 92.8-22,560 1:2 1:50 62
MMP-12 0.424 20.9-5,090 1:2 1:2 73
Nephrin 154 680-165,220 1:2 1:2 38
Oncostatin M 6.26 72.3-17,580 1:2 1:2 64
Osteopontin 2.37 36.4-8,840 1:2 1:50 56
Osteoprotegerin 6.32 48.9-11,890 1:2 1:2 39
Pancreatic Polypeptide 23.3 252-61,160 1:2 1:2 45
PDGF-AA 0.903 4.16-1,010 1:2 1:200/1:2 14
PDGF-AB 0.725 8.20-1,993 1:2 1:2 46
PDGF-BB 1.49 29.4-7,150 1:2 1:50 27
Periostin/OSF-2 201 1,370-332,890 1:2 1:200 67
PlGF-2 1.70 9.37-2,270 1:2 1:2 44
Podocalyxin 5.03 115-28,000 1:2 1:2 77
P-Selectin 2.60 138-33,580 1:2 1:2 46
Prolactin 2.67 64.0-15,550 1:2 1:2 44
Proprotein Convertase 9/ PCSK9 289 430-104,500 1:2 1:2 53
RAGE 18.0 54.3-13,200 1:2 1:2 78
Renin 13.2 129-31,300 1:2 1:2 47
Resistin 0.737 3.3-800 1:2 1:50 36
S100A8 61.3 278-67,490 1:2 1:2 20
S100A9 3.55 110-26,720 1:2 1:2 21
Serpin E1/PAI-1 5.71 30.2-7,340 1:2 1:50 43
SOST 2.27 16.3-3,950 1:2 1:2 48
SP-D 11.0 234-56,760 1:2 1:2 12
Syndecan-1/CD138 288 1,066-259,100 1:2 1:2 51
Thrombospondin-4 17.5 256-62,290 1:2 1:200 13
TIM-1/KIM-1 13.1 257-62,380 1:2 1:2 72
TIMP-1 12.4 59.1-14,350 1:2 1:2 45
TIMP-4 14.4 123-29,850 1:2 1:2 47
TNF-alpha 1.47 3.7-900 1:2 1:2 14
TNF RI 0.124 7.86-1,910 1:2 1:2 15
TNF RII 2.48 46.7-11,340 1:2 1:2 74
TRANCE/RANK L 7.51 59.1-14,370 1:2 1:2 25
TWEAK 0.184 2.88-700 1:2 1:2 53
uPAR 88.2 1,080-262,520 1:2 1:2 76
VEGF 3.96 4.7-1,130 1:2 1:2 21
VEGF R2/KDR 27.5 267-64,810 1:2 1:2 26

*A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

 

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

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Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Chronic features of allergic asthma are enhanced in the absence of resistin-like molecule-beta
    Authors: KS LeMessurie, M Palipane, M Tiwary, B Gavin, AE Samarasing
    Sci Rep, 2018;8(1):7061.
    Species: Mouse
    Sample Type: Tissue Homogenates
  2. Caspase-8 deficiency in mouse embryos triggers chronic RIPK1-dependent activation of inflammatory genes, independently of RIPK3
    Authors: TB Kang, JS Jeong, SH Yang, A Kovalenko, D Wallach
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Type: Plasma
  3. Synergistic actions of FGF2 and bone marrow transplantation mitigate radiation-induced intestinal injury
    Authors: BH Kim, HW Jung, SH Seo, H Shin, J Kwon, JM Suh
    Cell Death Dis, 2018;9(3):383.
    Species: Mouse
    Sample Type: Serum
  4. Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
    Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
    Oncotarget, 2018;9(1):293-305.
    Species: Mouse
    Sample Type: Peritoneal Lavage
  5. Leptin Signaling Mediates Obesity-associated CSC Enrichment and EMT in Preclinical TNBC Models
    Authors: LW Bowers, EL Rossi, SB McDonell, SS Doerstling, SA Khatib, CG Lineberger, JE Albright, X Tang, LA deGraffenr, SD Hursting
    Mol. Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Type: Serum
  6. Serum amyloid A3 is required for normal weight and immunometabolic function in mice
    Authors: JL Ather, ME Poynter
    PLoS ONE, 2018;13(2):e0192352.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  7. The yeast form of the fungus Candida albicans promotes persistence in the gut of gnotobiotic mice
    Authors: L Böhm, S Torsin, SH Tint, MT Eckstein, T Ludwig, JC Pérez
    PLoS Pathog., 2017;13(10):e1006699.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation
    Authors: J Deng, ES Wang, RW Jenkins, S Li, R Dries, K Yates, S Chhabra, W Huang, H Liu, AR Aref, E Ivanova, CP Paweletz, M Bowden, CW Zhou, GS Herter-Spr, JA Sorrentino, JE Bisi, PH Lizotte, AA Merlino, MM Quinn, LE Bufe, A Yang, Y Zhang, H Zhang, P Gao, T Chen, ME Cavanaugh, AJ Rode, E Haines, PJ Roberts, JC Strum, WG Richards, JH Lorch, S Parangi, V Gunda, GM Boland, R Bueno, S Palakurthi, GJ Freeman, J Ritz, WN Haining, NE Sharpless, H Arthanari, GI Shapiro, DA Barbie, NS Gray, KK Wong
    Cancer Discov, 2017;0(0):.
    Species: Mouse
    Sample Type: X Lavage Fluid
  9. Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia
    Authors: TS Kapellos, C Recio, DR Greaves, AJ Iqbal
    Mediators Inflamm., 2017;2017(0):4315412.
    Species: Mouse
    Sample Type: Serum
  10. Lactobacillus paracasei feeding improves immune control of influenza infection in mice
    Authors: N Belkacem, N Serafini, R Wheeler, M Derrien, L Boucinha, A Couesnon, N Cerf-Bensu, I Gomperts B, JP Di Santo, MK Taha, R Bourdet-Si
    PLoS ONE, 2017;12(9):e0184976.
    Species: Mouse
    Sample Type: Tissue Homogenates
  11. Lack of Histamine H4-Receptor Expression Aggravates TNBS-Induced Acute Colitis Symptoms in Mice
    Authors: EJ Wunschel, B Schirmer, R Seifert, D Neumann
    Front Pharmacol, 2017;8(0):642.
    Species: Mouse
    Sample Type: Tissue Homogenates
  12. Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
    Authors: SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn
    Stem Cell Res Ther, 2017;8(1):140.
    Species: Mouse
    Sample Type: Serum
  13. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas
    Int J Cancer, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  14. Nrf2 Modulates Host Defense during Streptococcus pneumoniae Pneumonia in Mice
    Authors: JC Gomez, H Dang, JR Martin, CM Doerschuk
    J. Immunol, 2016;197(7):2864-79.
    Species: Mouse
    Sample Type: BALF
  15. Positive Prehabilitative Effect of Intense Treadmill Exercise for Ameliorating Cancer Cachexia Symptoms in a Mouse Model
    J Cancer, 2016;7(15):2378-2387.
    Species: Mouse
    Sample Type: Plasma
  16. Factor XI deficiency enhances the pulmonary allergic response to house dust mite in mice independent of factor XII
    Authors: Ingrid Stroo
    Am. J. Physiol. Lung Cell Mol. Physiol, 2016;0(0):ajplung.00320.
    Species: Mouse
    Sample Type: Tissue Homogenates
  17. CF Lung Infection and Inflammation: IL-17 Pathophysiology and Therapeutic Intervention
    Authors: Daniel Hsu
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  18. Atypical chemokine receptor ACKR2 controls branching morphogenesis in the developing mammary gland
    Authors: Gerard J Graham
    Development, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  19. Complement Component 3 Regulates IFN-? Production by Plasmacytoid Dendritic Cells following TLR7 Activation by a Plant Virus-like Nanoparticle
    Authors: Alain Lamarre
    J. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  20. Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
    Nat Commun, 2016;7(0):12304.
    Species: Mouse
    Sample Type: Serum
Expand to show all Citations

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Average Rating: 5 (Based on 4 reviews)

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Excellent
  Anonymous 04/12/2018
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Sample Tested 4T1.2 mouse mammary carcinoma cells
Excellent
  Anonymous 04/10/2018
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Sample Tested 7TD1 mouse hybridoma cell line
Excellent
  Anonymous 04/05/2018
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Sample Tested Serum
 Mouse Magnetic Luminex Assay LXSAMSM-10 Array LXSAMSM
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  Anonymous 07/17/2017
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 Mouse Magnetic Luminex Assay LXSAMSM-10
: Mouse Magnetic Luminex Assay [LXSAMSM-10].

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Sample Tested Lung single-cell suspension

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