Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Culture, ELISA Detection, FACS

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805

Specificity

Detects mouse Mer in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Mer Biotinylated Antibody

Detection of Mer antibody in J774A.1 Mouse Cell line antibody by Flow Cytometry.

Detection of Mer in J774A.1 Mouse Cell line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF591, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Mer Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Mer Fc Chimera (Catalog # 591-MR)

Reviewed Applications

Read 7 reviews rated 4.1 using BAF591 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q60805

Additional Mer Products

Product Documents for Mouse Mer Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Mer Biotinylated Antibody

For research use only

Citations for Mouse Mer Biotinylated Antibody

Customer Reviews for Mouse Mer Biotinylated Antibody (7)

4.1 out of 5
7 Customer Ratings
5 Stars
14%
4 Stars
86%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 01/24/2023
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 01/24/2023
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 09/04/2022
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/04/2022
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 08/19/2022
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 04/01/2020
    Mouse Mer Biotinylated Antibody BAF591
  • Mouse Mer Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: PRIMARY MACROPHAGES
    Species: Mouse
    Verified Customer | Posted 07/03/2016

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