Mouse Mer Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF591
Detection  of Mer in J774A.1 Mouse Cell line by Flow Cytometry.
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Product Details
Citations (10)
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Reviews (1)

Mouse Mer Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mer in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Mer Fc Chimera (Catalog # 591-MR)
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Mer in J774A.1 Mouse Cell line by Flow Cytometry. View Larger

Detection of Mer in J774A.1 Mouse Cell line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF591, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Product Datasheets

Citations for Mouse Mer Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Conventional DCs from Male and Female Lupus-Prone B6.NZM Sle1/Sle2/Sle3 Mice Express an IFN Signature and Have a Higher Immunometabolism That Are Enhanced by Estrogen
    Authors: MH Lee, M Chakhtoura, U Sriram, R Caricchio, S Gallucci
    J Immunol Res, 2018;2018(0):1601079.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer
    Authors: AP Gounder, CC Yokoyama, NN Jarjour, TL Bricker, BT Edelson, ACM Boon
    PLoS Pathog., 2018;14(4):e1007001.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. A Discrete Subset of Monocyte-Derived Cells among Typical Conventional Type 2 Dendritic Cells Can Efficiently Cross-Present
    Authors: J Sheng, Q Chen, I Soncin, SL Ng, K Karjalaine, C Ruedl
    Cell Rep, 2017;21(5):1203-1214.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
    Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  5. Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
    Authors: Olga Barreiro
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow
  6. Lymph-borne CD8alpha+ dendritic cells are uniquely able to cross-prime CD8+ T cells with antigen acquired from intestinal epithelial cells.
    Authors: Cerovic V, Houston S, Westlund J, Utriainen L, Davison E, Scott C, Bain C, Joeris T, Agace W, Kroczek R, Mowat A, Yrlid U, Milling S
    Mucosal Immunol, 2015;8(1):38-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  7. DNA-Mediated Cyclic GMP-AMP Synthase-Dependent and -Independent Regulation of Innate Immune Responses.
    Authors: Motani K, Ito S, Nagata S
    J Immunol, 2015;194(10):4914-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  8. MerTK-mediated engulfment of pyrenocytes by central macrophages in erythroblastic islands.
    Authors: Toda S, Segawa K, Nagata S
    Blood, 2014;123(25):3963-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: FACS sorting
  9. Distinct development and functions of resident and recruited liver Kupffer cells/macrophages.
    Authors: Ikarashi M, Nakashima H, Kinoshita M, Sato A, Nakashima M, Miyazaki H, Nishiyama K, Yamamoto J, Seki S
    J Leukoc Biol, 2013;94(6):1325-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  10. The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung.
    Authors: Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T
    Mucosal Immunol, 0;8(5):1021-30.
    Species: Mouse
    Sample Types: BALF
    Applications: Flow

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Mouse Mer Biotinylated Antibody
By Anonymous on 07/03/2016
Application: Flow Sample Tested: PRIMARY MACROPHAGES Species: Mouse