Mouse Meteorin Antibody Summary
Accession # Q8C1Q4
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Meteorin Monoclonal Antibody (Catalog # MAB34752).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Meteorin DuoSet ELISA Kit (Catalog # DY3475) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse Meteorin ELISA Standard Curve. Recombinant Mouse Meteorin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Meteorin Monoclonal Antibody (Catalog # MAB34752) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Meteorin Monoclonal Antibody (Catalog # MAB34751) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Meteorin is a 31 kDa, secreted protein that is expressed by undifferentiated neural and glial progenitors, and astrocytes. Its name derives from it ability to generate glial cells with long meteor-like tails. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor that contains a 270 aa mature region. It contains eight cysteines with no identifiable domains or N-linked glycosylation sites. There is one alternate splice form where the first 164 aa of the precursor are deleted and aa 165 is changed to a Met, possibly to serve in translation initiation. Mature mouse Meteorin is 82% and 77% aa identical to human and bovine Meteorin, respectively.
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