Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all MOG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MOG
Gly29-Gly153
Accession # Q61885

Specificity

Detects mouse MOG in Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human MOG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse MOG Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebral cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MOG

Reviewed Applications

Read 1 review rated 4 using BAF2439 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MOG

Mouse MOG is an integral membrane protein belonging to the immunoglobulin superfamily. It is found exclusively on myelinating oligodendrocytes in the central nervous system. The amino-terminal extracellular domain of mouse MOG shares 97% and 92% amino acid sequence identity with the corresponding regions of rat and human MOG, respectively.

Long Name

Myelin Oligodendrocyte Glycoprotein

Alternate Names

MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein

Entrez Gene IDs

4340 (Human); 17441 (Mouse)

Gene Symbol

MOG

UniProt

Q61885

Additional MOG Products

Product Documents for Mouse MOG Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MOG Biotinylated Antibody

For research use only

Citations for Mouse MOG Biotinylated Antibody

Customer Reviews for Mouse MOG Biotinylated Antibody (1)

4 out of 5
1 Customer Rating
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  • Mouse MOG Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 02/07/2019
    CNS cells of EAE mice stained for viable cells, MOG (conjugated with streptavidin-BV786), O4, GalC and AN2. Non-stained cells (blue) and stained cells (red).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for MOG, O4, GalC and AN2, plus for viability to exclude dead cells. MOG antibody was biotinylated, so cells were subsequently incubated with streptavidin-BV786 to stain MOG+ cells. Viable cells were gated and MOG staining is showed in non-stained cells (blue histogram) and in stained cells (red histogram).
    Mouse MOG Biotinylated Antibody BAF2439

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Protocols

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