Mouse NFIL3/E4BP4 PE-conjugated Antibody

IC8888P has been discontinued. View all NFIL3/E4BP4 products.
Recombinant Monoclonal Antibody
  
Product Details
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse NFIL3/E4BP4 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1218A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse NFIL3/E4BP4
    Lys140-Arg462
    Accession # O08750
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) untreated or (B) treated with 100 ng/mL Recombinant Mouse IL‑2 (Catalog # 402-ML) overnight were stained with Rabbit Anti-Mouse NFIL3/E4BP4 PE‑conjugated Monoclonal Antibody (Catalog # IC8888P) and Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A). Quadrant markers were set based on control antibody staining (Catalog # IC105P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NFIL3/E4BP4
NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor belonging to the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in Th2 cells, Th1 cells, pro-B lymphocytes and cardiomyocytes. NFIL3 binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.
  • Long Name:
    Nuclear Factor, Interleukin 3 Regulated
  • Entrez Gene IDs:
    4783 (Human); 18030 (Mouse); 114519 (Rat)
  • Alternate Names:
    E4BP4; E4BP4IL3BP1; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL3; NF-IL3A; NFIL3AE4 promoter-binding protein 4; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A
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IC8888P has been discontinued.

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