Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes either (A) untreated or (B) treated with 100 ng/mL Recombinant Mouse IL‑2 (Catalog # 402-ML) overnight were stained with Rabbit Anti-Mouse NFIL3/E4BP4 PE‑conjugated Monoclonal Antibody (Catalog # IC8888P) and Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A). Quadrant markers were set based on control antibody staining (Catalog # IC105P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor belonging to the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in Th2 cells, Th1 cells, pro-B lymphocytes and cardiomyocytes. NFIL3 binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.
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