Mouse Notch-3 Antibody

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Product Details
Citations (5)
Supplemental Products

Mouse Notch-3 Antibody Summary

Species Reactivity
Detects mouse Notch‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human
Notch‑3 is observed and less than 5% cross-reactivity with recombinant rat (rr) Notch-1 and rrNotch-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Notch-3
Accession # Q61982
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Notch‑3 Fc Chimera, aa 40-468 (Catalog # 1308-NT)
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) to immobilized Recombinant Mouse Notch-3 (Catalog # 1308-NT) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-3

Mouse Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2318 amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 20 aa transmembrane (TM) segment and a 655 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.

Notch-3 is predominantly expressed in the developing central nervous system of mice (2). Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Mouse Notch-3 shows 90% aa identity to human Notch-3 and 96% to rat Notch-3 over the entire protein.

  1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
  2. Lardelli, M. et al. (1994) Mech Dev. 46:123.
  3. Rebay, I. et al. (1991) Cell 67:687.
  4. Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
  5. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
Entrez Gene IDs
4854 (Human); 18131 (Mouse)
Alternate Names
CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3

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Citations for Mouse Notch-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Notch signalling drives synovial fibroblast identity and arthritis pathology
    Authors: K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin,, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner
    Nature, 2020;582(7811):259-264.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Involvement of Notch2 in all?trans retinoic acid?induced inhibition of mouse embryonic palate mesenchymal cell proliferation
    Authors: Y Zhang, S Dong, J Wang, M Wang, M Chen, H Huang
    Mol Med Rep, 2017;16(3):2538-2546.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Canonical notch signaling functions as a commitment switch in the epidermal lineage.
    Authors: Blanpain C, Lowry WE, Pasolli HA, Fuchs E
    Genes Dev., 2006;20(21):3022-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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