Mouse PD-ECGF/Thymidine Phosphorylase Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-ECGF/Thymidine Phosphorylase
    Met1-Pro471, predicted
    Accession # Q99N42
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Mouse PD-ECGF/Thymidine Phosphorylase by Western Blot. Western blot shows lysates of L1210 mouse lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PD‑ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PD‑ECGF/Thymidine Phosphorylase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-ECGF/Thymidine Phosphorylase

TYMP (Thymidine phosphorylase/TP; also [PD]-ECGF/platelet-derived endothelial cell growth factor, gliostatin and TdRPase) is a 50-55 kDa member of the pyrimidine-nucleoside phosphorylase family of enzymes. TYMP/TP is both a cytosolic and secreted molecule that is expressed by a variety of cell types, including, macrophages, hepatocytes, endometrial gland epithelium, vascular smooth muscle and endothelial cells. It has also been found in select tumor cell types, and via an angiogenic activity, has been proposed to promote tumor growth. TYMP converts thymidine to thymine and 2-deoxy-ribose-1P, and it is the 2-deoxy-ribose component that is believed to promote endothelial cell migration (but not proliferation). This may be due to the fact that 2-deoxy-ribose induces reactive oxygen species, which drive the production of angiogenic factors, and that 2-deoxy-ribose also activates endothelial cell integrins. Mouse TYMP is 471 amino acids (aa) in length. It contains a phosphorylase domain (aa 96-348), followed by a C-terminal region (aa 374-448). Although TYMP circulates, there is no definitive signal sequence. TYMP is known to form homodimers. Full-length mouse TYMP (aa 1-471) shares 80% and 92% aa sequence identity with human and rat TYMP, respectively.

  • Long Name:
    Platelet-derived Endothelial Cell Growth Factor
  • Entrez Gene IDs:
    1890 (Human); 72962 (Mouse)
  • Alternate Names:
    dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 17  
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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