Mouse PDGF R alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1062
PDGF R alpha  in Mouse Embryo.
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Product Details
Citations (1)
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Mouse PDGF R alpha Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGF R alpha in Western blots. In Western blots, less than 15% cross-reactivity with recombinant human PDGF R alpha is observed and less than 1% cross-reactivity with recombinant mouse PDGF R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PDGF R alpha Fc Chimera (Catalog # 1062-PR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PDGF Ra antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PDGF R alpha in Mouse Embryo. PDGF Ra was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse PDGF Ra Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero‑dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero‑dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB, and -DD (1‑4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand‑induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

References
  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citation for Mouse PDGF R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Contribution of neural crest-derived cells in the embryonic and adult thymus.
    Authors: Foster K, Sheridan J, Veiga-Fernandes H, Roderick K, Pachnis V, Adams R, Blackburn C, Kioussis D, Coles M
    J. Immunol., 2008-03-01;180(5):3183-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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