Mouse PDGF R alpha Antibody

Catalog # Availability Size / Price Qty
AF1062
AF1062-SP

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Detection of Mouse PDGF R alpha  by Western Blot.
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Mouse PDGF R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
3T3-L1 mouse embryonic fibroblast adipose-like cell line
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2 - 1.6 µg/mL in the presence of 250 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PDGF Ra antibody by Western Blot. View Larger

Detection of Mouse PDGF R alpha by Western Blot. Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for PDGF Ra at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PDGF Ra antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PDGF R alpha in Mouse Embryo. PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry PDGF Ra antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PDGF R alpha in Mouse Embryo. PDGF Ra was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha  Antibody. View Larger

Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF-AA (221-AA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002. Proliferation elicited by Recombinant Human PDGF-AA (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 0.2-1.6 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of Rat PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Rat PDGF R alpha by Immunocytochemistry/Immunofluorescence. Extracellularly applied recombinant human alpha -syn PFFs induced cytoplasmic alpha -syn-immunoreactive inclusions in primary BCAS1(+) cell cultures. Immunostaining of oligodendroglial cells incubated with 1 μM alpha -syn PFFs for 24 h from days 3 (upper) and 4 (lower) after differentiation induction showing the ubiquitous development of thioflavin S-labeled inclusions in PDGFR alpha (+) cells and BCAS1(+) cells. In contrast, few BCAS1(−)/MBP(+) cells developed thioflavin S-labeled inclusions. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32727582), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the mouse VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark VTTs cell bodies and telopodes. Scale bar–10 µm, in inset, green arrows point to Rspo3 mRNAs (green dots) localized on PDGFRa telopodes (blue). Telocyte cell body is marked by white dashed line. inset Scale bar–5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Leptin promotes OPC proliferation. Representative image of cultured OPCs stained with antibodies against LepRb (green) and PDGFR alpha (red). Scale bar: 25 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. LPC injection does not enhance leptin expression in the CNS. Representative images of LepRb (green) expression in combination with PDGFR alpha, GFAP NeuN, and CD11b (red) in the mouse spinal cord with or without LPC injection. Spinal cord sections were obtained 3 days after LPC injection. Graph indicates the relative intensity of leptin protein expression in indicated cell type (n = 3). P = 0.287452 (PDGFR alpha ), 0.181059 (GFAP), 0.199972 (NeuN), Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bar: 25 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY licence.

Immunohistochemistry Detection of Mouse PDGF R alpha by Immunohistochemistry. View Larger

Detection of Mouse PDGF R alpha by Immunohistochemistry. PDGFR alpha driven mouse brain tumor model. Example of early stage tumor growth, as revealed by IHC for proliferation marker Ki67 and PDGFR alpha. Note high density of Ki67+ proliferating cells in tumor area, increased expression level of PDGFR alpha, and invasive migration of tumor cells through corpus callosum into contralateral hemisphere. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. OPC expresses leptin receptors. Representative images of mouse spinal cord sections, which were double-labeled for LepRb (green) in combination with PDGFR alpha (red). Spinal cord sections were obtained 7 days after LPC injection; the graph shows quantification (n = 3). P = 0.007573 (LepRb flox vs intact CKO), 0.0108779 (LepRb flox vs LPC CKO), ANOVA with Tukey’s post-hoc test. Scale bar: 25 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Endogenous leptin sustains spontaneous OPC proliferation. Representative images of mouse spinal cord sections, which were prepared 7 days (left panels) and 14 days (right panels) after LPC injection and double labeled for BrdU in combination with PDGFR alpha (upper panels), GSTπ (upper panels) and olig2 (lower panels). BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n = 5–8). P = 0.042915 (PDGFR alpha and BrdU labeled cells), 0.013560 (Olig2 and BrdU labeled cells 7 days after injection), 0.012111 (GSTπ and BrdU labeled cells), 0.009797 (Olig2 and BrdU labeled cells 14 days after injection), Student’s t-test. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Etv5 is necessary and sufficient for proliferation and cell fate bias downstream of Cic loss. Cic-null mouse NSCs (CicnullEmpty), Cic-null mouse NSCs with dominant negative Etv5 (CicnullDN-Etv5), and Cic-wildtype mouse NSCs overexpressing Etv5 (Etv5 overpression) were grown in lineage-directed culture conditions and assessed for their ability to differentiate to oligodendrocytes as determined by immunostaining for Olig2, Pdgfra, and Mbp. Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043608), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Transplantation of CD11b/LIF transgenic BMCs reduces the numbers of FAPs in dystrophic muscle but does not affect phenotype. To quantify the number of FAPs, transgenic mouse muscle sections were co-labeled with antibodies to PDGFR alpha (red) and CD31, CD45 (green). Arrowheads indicate FAPs (CD31-CD45-PDGFR alpha +). Bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31243277), licensed under a CC-BY licence.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. View Larger

Detection of Mouse PDGF R alpha by Immunocytochemistry/Immunofluorescence. Lgr5 is expressed abundantly in mouse villus tip telocytes. d) Lgr5 mRNA (red dots) expressed in PDGFRa+ VTTs that co-express Bmp4 mRNA (green dots). Scale bar–10 µm. Red arrows point to Lgr5 and Bmp4 double positive cells. e) Blow up of the region boxed in d). Scale bar–5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY licence.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumor model.(a) Schematic diagram of PDGFR alpha J/K knock-in alleles. ATG, start codon; SA, splice acceptor; STOP, PGK-neo cassette. (b) Kaplan-Meier survival curves of 4 mouse mutant cohorts with brain tumors. Mice generally succumbed to subcutaneous fibrosarcomas, and brain tumors were detected by histological analysis. (c) Example of early stage tumor growth, as revealed by IHC for proliferation marker Ki67 and PDGFR alpha. Note high density of Ki67+ proliferating cells in tumor area, increased expression level of PDGFR alpha, and invasive migration of tumor cells through corpus callosum into contralateral hemisphere. (d) H&E staining of an advanced brain tumor growth (asterisk in tumor centre, dashed line demarcates expansion). Scale bars: 50 μm (c, d). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Etv5 is necessary and sufficient for proliferation and cell fate bias downstream of Cic loss. a–f Cic-null NSCs (CicnullEmpty), Cic-null NSCs with dominant negative Etv5 (CicnullDN-Etv5), and Cic-wildtype NSCs overexpressing Etv5 (Etv5 overpression) were grown in lineage-directed culture conditions and assessed for their ability to differentiate to neurons, astrocytes, and oligodendrocytes as determined by immunostaining for bIII-Tubulin (Tuj1), Gfap, and Olig2, Pdgfra, and Mbp. Scale bar: 10 μm. Analysis of Tuj1 + cells from NSCs in neuronal differentiating condition, NDC a, d; analysis of Gfap + cells from NSCs in astrocytic differentiating condition, ADC b, e; and analyses of Olig2 + , Pdgfra + , and Mbp + cells from NSCs in oligodendrocyte differentiating condition, ODC c, f from n = 3 biological replicates, with three technical replicates each, for cell culture studies. g, h Representative images and quantitation of EdU incorporation 2 days post electroporation of wildtype ETV5 or empty control plasmid, both carrying mCherry as a marker, into E13 CICFl/Fl VZ. Note: mCherry fluorescence and EdU staining were false-colorized to green and red after grayscale imaging. Scale bar: 50 μm. Data from n = 4 mice per each group. Scale bar: 50 μm. i, j Representative images and quantitation of EdU incorporation 2 days post-electroporation of Cre only or of Cre co-electroporated with DNETV5 into E13 CICFl/Fl VZ. Data from n = 4 mice per each group. Scale bar: 50 μm k EdU incorporation assay in cultured Cic-wildtype NSCs without or with ETV5 overexpression from n ≥ 3 biological replicates. l EdU incorporation in Cic-floxed NSCs with Cre, and without or with DNETV5 expression from n ≥ 3 biological replicates. Data shown as mean ± SD. Statistical analyses performed either t test in h, j, k, l; or with ANOVA with Tukey’s post hoc test in d, e, f. ns–not significant, *p < 0.05, **p < 0.01, ***p < 0.0001. Source data are provided as a Source Data file. ADC–astrocytic differentiation condition, NDC–neuronal differentiation condition, ODC–oligodendrocytic differentiation condition. VZ–ventricular zone, LV–lateral ventricle Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31043608), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence LPC injection does not enhance leptin expression in the CNS.(a) Representative images of MBP expression in a mouse spinal cord 14 days after LPC injection are shown; the graph shows quantification of the demyelinating area in the dorsal spinal cord (n = 3–4). P = 0.001542, Student’s t-test. (b) Representative images of NeuN expression in a mouse spinal cord 14 days after LPC injection; the graph shows quantification of the density of NeuN-positive cells in the spinal cord (n = 3). P = 0.299940, Student’s t-test, n.s. indicates no significant difference. (c) Quantification of leptin protein expression in indicated organs. Tissues were obtained from the mice 3 days after LPC injection (n = 3 for control, 4 for LPC injection). P = 0.318966 (adipose tissue), 0.10446 (brain stem), 0.332281 (cerebellum), 0.345245 (liver), 0.453104 (kidney), 0.098135 (heart), 0.335722 (lung), 0.236771 (muscle), 0.44662 (spleen), 0.465966 (stomach). Student’s t-test. n.s. indicates no significant difference. (d) Quantification of spinal cord leptin protein 3 days after LPC injection (n = 3 for control, 4 for LPC injection). P = 0.026865, Student’s t-test. (e) Quantification of spinal cord leptin mRNA 3 days after LPC injection (n = 6). P = 0.324930, Student’s t-test, n.s. indicates no significant difference. (f) Representative images of LepRb (green) expression in combination with PDGFR alpha, GFAP NeuN, and CD11b (red) in the mouse spinal cord with or without LPC injection. Spinal cord sections were obtained 3 days after LPC injection. Graph indicates the relative intensity of leptin protein expression in indicated cell type (n = 3). P = 0.287452 (PDGFR alpha ), 0.181059 (GFAP), 0.199972 (NeuN), Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bars; 100 μm for (a and b), 25 μm for (f). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Leptin promotes OPC proliferation.(a) Representative image of cultured OPCs stained with antibodies against LepRb (green) and PDGFR alpha (red). Scale bar: 25 μm. (b) Relative BrdU incorporation into the OPC obtained from the brain (left graph) and spinal cord (right graph). Cells were treated with recombinant leptin for 48 h (n = 4). (Left graph) P = 0.005993 (control vs 10 ng/mL), 0.045616 (control vs 100 ng/mL), (Right graph) P = 0.004456 (control vs 10 ng/mL), 0.017859 (control vs 100 ng/mL). (c) Relative BrdU incorporation into the OPC after leptin stimulation (10 ng/ml) with U0126 (20 μM), a MEK inhibitor (n = 4 for brain OPCs, n = 3 for spinal cord OPCs). (Left graph) P = 0.019753 (control vs leptin), 0.039433 (leptin vs leptin + U0126), (Right graph) P = 0.045545 (control vs leptin), 0.04486 (leptin vs leptin + U0126). (d) Representative images of western blotting (upper panels) and quantitative analysis of ERK phosphorylation (lower graph) are shown. OPCs were treated with leptin (10 ng/ml) under indicated periods (n = 3). P = 0.006352 (2 min), 0.016571 (5 min), 0.017675 (10 min), 0.024100 (15 min), 0.081342 (30 min). *P < 0.05, **P < 0.01, ANOVA with Tukey’s post-hoc test. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Rat PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Rat PDGFR alpha by Immunocytochemistry/Immunofluorescence Extracellularly applied recombinant human alpha -syn PFFs induced cytoplasmic alpha -syn-immunoreactive inclusions in primary BCAS1(+) cell cultures. a Confocal images of BCAS1(+) cells, which were incubated with 1 μM alpha -syn PFFs for 24 h from day 4 after differentiation induction, showing the intracellular inclusions labeled with both anti-alpha -syn antibody and thioflavin S. Scale bar = 5 μm. b Immunostaining of oligodendroglial cells incubated with 1 μM alpha -syn PFFs for 24 h from days 3 (upper) and 4 (lower) after differentiation induction showing the ubiquitous development of thioflavin S-labeled inclusions in PDGFR alpha (+) cells and BCAS1(+) cells. In contrast, few BCAS1(−)/MBP(+) cells developed thioflavin S-labeled inclusions. Scale bar = 50 μm. c The percentages of oligodendroglial cells containing thioflavin S-labeled inclusions were compared between BCAS1(−)/PDGFR alpha (+) cells and BCAS1(+)/PDGFR alpha (+) cells (upper, performed on day 3), and between BCAS1(+)/MBP(+) cells and BCAS1(−)/MBP(+) cells (lower, performed on day 4). N = 4, respectively, independent culture, Mann–Whitney, p* < 0.05 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32727582), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Transplantation of CD11b/LIF transgenic BMCs reduces the numbers of FAPs in dystrophic muscle but does not affect phenotype. a QPCR analysis shows that TA muscles from LIF BMT/mdx recipients have reduced Pdgfra gene expression. N = 7 or 8 for WT BMT/mdx and LIF BMT/mdx data sets, respectively, * indicates significantly different from WT BMT/mdx recipients at P < 0.05. P-values based on two-tailed t-test. For all histograms in the figure, the bars indicate mean ± sem. b To quantify the number of FAPs, muscle sections were co-labeled with antibodies to PDGFR alpha (red) and CD31, CD45 (green). Arrowheads indicate FAPs (CD31-CD45-PDGFR alpha +). Bar = 50 μm. c Fewer FAPs (CD31-CD45-PDGFR alpha +) in TA cross-sections of LIF BMT/mdx recipients compared to WT BMT/mdx recipients. N = 5 for each data set. d There was no detectible change in phenotype of PDGFR alpha + cells assayed for co-expression of the fibrogenic marker HSP47. e FACS plots demonstrating strategy for sorting FAPs (Hoechst + CD11b-CD31-CD45-PDGFR alpha +). Fibroblasts derived from FAPs were stimulated with LIF (10 ng/ml) and/or TGF beta 1 (10 ng/ml) for 3 h (f–h) or 3 days (i–k) and assayed by QPCR for Ctgf (f, i), Fn1 (g, j), and Col1a1 (h, k). N = 4 technical replicates for each data set. Significant findings were verified with biological replicates of cells sorted from independent donors. * Indicates significantly different from control cultures, # indicates significantly different from TGF beta 1 treated cultures, and Φ indicates significantly different from LIF-treated cultures at P < 0.05. P-values based on ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31243277), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Lgr5 is expressed abundantly in villus tip telocytes.a smFISH of Lgr5, DAPI in blue, Scale bar–20 µm. b Blow up of villus tip, Scale bar–10 µm. In a, b thin white arrows point at autofluorescent blobs. c blow up of crypt, Scale bar–10 µm. Red arrows in b–c point to Lgr5 positive cells. dLgr5 mRNA (red dots) expressed in PDGFRa+ VTTs that co-express Bmp4 mRNA (green dots). Scale bar–10 µm. Red arrows point to Lgr5 and Bmp4 double positive cells. e Blow up of the region boxed in d. Scale bar–5 µm. fLgr5 mRNA concentrations in VTTs are comparable to those in Lgr5+ crypt base columnar cells (n = 25 cells examined over 2 mice for each region). Boxes show 25–75 percentiles of the smFISH expression, horizontal red lines are medians. Whiskers, extend to the most extreme data point within 1.5× the interquartile range (IQR) from the box; g) Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark VTTs cell bodies and telopodes. Scale bar–10 µm, in inset, green arrows point to Rspo3 mRNAs (green dots) localized on PDGFRa telopodes (blue). Telocyte cell body is marked by white dashed line. inset Scale bar–5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Lgr5 is expressed abundantly in villus tip telocytes.a smFISH of Lgr5, DAPI in blue, Scale bar–20 µm. b Blow up of villus tip, Scale bar–10 µm. In a, b thin white arrows point at autofluorescent blobs. c blow up of crypt, Scale bar–10 µm. Red arrows in b–c point to Lgr5 positive cells. dLgr5 mRNA (red dots) expressed in PDGFRa+ VTTs that co-express Bmp4 mRNA (green dots). Scale bar–10 µm. Red arrows point to Lgr5 and Bmp4 double positive cells. e Blow up of the region boxed in d. Scale bar–5 µm. fLgr5 mRNA concentrations in VTTs are comparable to those in Lgr5+ crypt base columnar cells (n = 25 cells examined over 2 mice for each region). Boxes show 25–75 percentiles of the smFISH expression, horizontal red lines are medians. Whiskers, extend to the most extreme data point within 1.5× the interquartile range (IQR) from the box; g) Rspo3 mRNAs are localized on telopodes that extend away from the cell bodies of the VTTs. VTTs are marked by Lgr5 mRNA (red dots), Rspo3 mRNA (green dots) is localized away from the cell body, PDGFRa antibody mark VTTs cell bodies and telopodes. Scale bar–10 µm, in inset, green arrows point to Rspo3 mRNAs (green dots) localized on PDGFRa telopodes (blue). Telocyte cell body is marked by white dashed line. inset Scale bar–5 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32321913), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence OPC expresses leptin receptors.(a) Representative images of spinal cord sections, which were double-labeled for LepRb (green) in combination with PDGFR alpha (red). Spinal cord sections were obtained 7 days after LPC injection; the graph shows quantification (n = 3). P = 0.007573 (LepRb flox vs intact CKO), 0.0108779 (LepRb flox vs LPC CKO), ANOVA with Tukey’s post-hoc test. (b) Relative expression of leptin receptors mRNA in PDGFR alpha -positive OPC obtained from the brain of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice (n = 5,6). P = 0.005878 (LepRa), 0.010306 (LepRb), 0.001535 (LepRc), 0.003169 (LepRd), 0.030459 (LepRe), Student’s t-test. (c) Representative images of spinal cord sections which were double labeled for BrdU in combination with PDGFR alpha (left panels) and GSTπ (right panels). Sections were prepared 7 days (left panels) and 14 days (right panels) after LPC injection. BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n = 5–8). P = 0.029791(PDGFR alpha and BrdU labeled cells), 0.028870 (GSTπ and BrdU labeled cells), Student’s t-test. (d) Representative images of PDGFR alpha expression in the intact spinal cord of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice; the graph shows quantification (n = 3–4). P = 0.404999, Student’s t-test, n.s. indicates no significant difference. (e) Representative images of APC expression in the intact spinal cord of PDGFR alpha -creERT:: Lepr flox/flox mice and +/+::Lepr flox/flox mice; the graph shows quantification (n = 3). P = 0.495667, Student’s t-test, n.s. indicates no significant difference. (f) Representative spinal cord section of PDGFR alpha -creERT:: Lepr flox/flox mice, which were prepared 14 days after LPC injection and stained with MBP; the graph shows quantification of the demyelinating area in the dorsal spinal cord (n = 7 for control, 10 for CKO). P = 0.030688, Student’s t-test. (g) Representative spinal cord sections which were labeled for CD11b. Sections were prepared 7 days after LPC injection. The graph shows quantification (n = 3). P = 0.493264, Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bars: 25 μm for (a), 50 μm for high magnification images in (c), 100 μm for others. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PDGFR alpha by Immunocytochemistry/Immunofluorescence Endogenous leptin sustains spontaneous OPC proliferation.(a) Representative images of spinal cord sections, which were prepared 7 days (left panels) and 14 days (right panels) after LPC injection and double labeled for BrdU in combination with PDGFR alpha (upper panels), GSTπ (upper panels) and olig2 (lower panels). BrdU was administrated during 3–7 days after LPC injection; the graph shows quantification (n = 5–8). P = 0.042915 (PDGFR alpha and BrdU labeled cells), 0.013560 (Olig2 and BrdU labeled cells 7 days after injection), 0.012111 (GSTπ and BrdU labeled cells), 0.009797 (Olig2 and BrdU labeled cells 14 days after injection), Student’s t-test. (b) Representative spinal cord sections, which were prepared 14 days after LPC injection and stained with MBP are shown; the graph shows quantification (n = 6). P = 0.025243, Student’s t-test. (c) Representative images of spinal cord section, which were prepared 7 days after LPC injection and labeled for CD11b; the graph shows quantification (n = 4). P = 0.213763, Student’s t-test, n.s. indicates no significant difference. *P < 0.05, **P < 0.01, error bars represent SEM. Scale bar: 50 μm for high magnification images in a, 100 μm for others. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep40397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse PDGFR alpha by Immunohistochemistry View Larger

Detection of Mouse PDGFR alpha by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry View Larger

Detection of PDGF R alpha in 3T3-L1 cells by Flow Cytometry 3T3-L1 cells were stained with Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

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Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

References
  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citations for Mouse PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

145 Citations: Showing 1 - 10
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  1. Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
    Authors: Nguyen, LT;Aprico, A;Nwoke, E;Walsh, AD;Blades, F;Avneri, R;Martin, E;Zalc, B;Kilpatrick, TJ;Binder, MD;
    Journal of neuroinflammation
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Dicer deficiency in microglia leads to accelerated demyelination and failed remyelination
    Authors: Tripathi, A;Rai, N;Perles, A;Jones, C;Dutta, R;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Spatial-temporal proliferation of hepatocytes during pregnancy revealed by genetic lineage tracing
    Authors: He, S;Guo, Z;Zhou, M;Wang, H;Zhang, Z;Shi, M;Li, X;Yang, X;He, L;
    Cell stem cell
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. IL-6 trans-signaling in a humanized mouse model of scleroderma
    Authors: Odell, ID;Agrawal, K;Sefik, E;Odell, AV;Caves, E;Kirkiles-Smith, NC;Horsley, V;Hinchcliff, M;Pober, JS;Kluger, Y;Flavell, RA;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC
  5. Emergent mechanical control of vascular morphogenesis
    Authors: Whisler, J;Shahreza, S;Schlegelmilch, K;Ege, N;Javanmardi, Y;Malandrino, A;Agrawal, A;Fantin, A;Serwinski, B;Azizgolshani, H;Park, C;Shone, V;Demuren, OO;Del Rosario, A;Butty, VL;Holroyd, N;Domart, MC;Hooper, S;Szita, N;Boyer, LA;Walker-Samuel, S;Djordjevic, B;Sheridan, GK;Collinson, L;Calvo, F;Ruhrberg, C;Sahai, E;Kamm, R;Moeendarbary, E;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  6. Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation
    Authors: Tsutsumi, R;Ueberheide, B;Liang, FX;Neel, BG;Sakai, R;Saito, Y;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. A cellular and molecular spatial atlas of dystrophic muscle
    Authors: Stec, MJ;Su, Q;Adler, C;Zhang, L;Golann, DR;Khan, NP;Panagis, L;Villalta, SA;Ni, M;Wei, Y;Walls, JR;Murphy, AJ;Yancopoulos, GD;Atwal, GS;Kleiner, S;Halasz, G;Sleeman, MW;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Spinal Cord Blood Vessels in Aged Mice Show Greater Levels of Hypoxia-Induced Vascular Disruption and Microglial Activation
    Authors: Halder, SK;Milner, R;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Transcriptome analysis of mesenchymal stromal cells of the large and small intestinal smooth muscle layers reveals a unique gastrontestinal stromal signature
    Authors: Chaen, T;Kurosawa, T;Kishi, K;Kaji, N;Ikemoto-Uezumi, M;Uezumi, A;Hori, M;
    Biochemistry and biophysics reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Patterning and folding of intestinal villi by active mesenchymal dewetting
    Authors: Huycke, TR;Miyazaki, H;Häkkinen, TJ;Srivastava, V;Barruet, E;McGinnis, CS;Kalantari, A;Cornwall-Scoones, J;Vaka, D;Zhu, Q;Jo, H;DeGrado, WF;Thomson, M;Garikipati, K;Boffelli, D;Klein, OD;Gartner, ZJ;
    bioRxiv : the preprint server for biology
    Species: Xenograft
    Sample Types: Organoid
    Applications: Bioassay
  11. Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration
    Authors: Norris, AM;Appu, AB;Johnson, CD;Zhou, LY;McKellar, DW;Renault, MA;Hammers, D;Cosgrove, BD;Kopinke, D;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
    Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
    Cell reports
  13. Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme
    Authors: Alber, AB;Marquez, HA;Ma, L;Kwong, G;Thapa, BR;Villacorta-Martin, C;Lindstrom-Vautrin, J;Bawa, P;Wang, F;Luo, Y;Ikonomou, L;Shi, W;Kotton, DN;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Organoid
    Applications: IHC
  14. PBAF Subunit Pbrm1 Selectively Influences the Transition from Progenitors to Pre-Myelinating Cells during Oligodendrocyte Development
    Authors: Waldhauser, V;Baroti, T;Fröb, F;Wegner, M;
    Cells
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Wound infiltrating adipocytes are not myofibroblasts
    Authors: Kalgudde Gopal, S;Dai, R;Stefanska, AM;Ansari, M;Zhao, J;Ramesh, P;Bagnoli, JW;Correa-Gallegos, D;Lin, Y;Christ, S;Angelidis, I;Lupperger, V;Marr, C;Davies, LC;Enard, W;Machens, HG;Schiller, HB;Jiang, D;Rinkevich, Y;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. SUMOylation of PDGF receptor ? affects signaling via PLC? and STAT3, and cell proliferation
    Authors: Wang, K;Papadopoulos, N;Hamidi, A;Lennartsson, J;Heldin, CH;
    BMC molecular and cell biology
    Species: African Green Monkey, Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Immunoprecipitation, IHC, Western Blot
  17. Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury
    Authors: Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ;
    Cell reports
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. The P-body protein 4E-T represses translation to regulate the balance between cell genesis and establishment of the postnatal NSC pool
    Authors: A Kolaj, SK Zahr, BS Wang, T Krawec, H Kazan, G Yang, DR Kaplan, FD Miller
    Cell Reports, 2023-03-15;42(3):112242.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Structure, signals, and cellular elements of the mouse gastric mesenchymal niche
    Authors: E Manieri, G Tie, D Seruggia, S Madha, A Maglieri, K Huang, Y Fujiwara, K Zhang, S Orkin, R He, N McCarthy, RA Shivdasani
    bioRxiv : the preprint server for biology, 2023-02-24;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: IHC
  20. Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner
    Authors: K Hoeft, GJL Schaefer, H Kim, D Schumacher, T Bleckwehl, Q Long, BM Klinkhamme, F Peisker, L Koch, J Nagai, M Halder, S Ziegler, E Liehn, C Kuppe, J Kranz, S Menzel, I Costa, A Wahida, P Boor, RK Schneider, S Hayat, R Kramann
    Cell Reports, 2023-02-17;42(2):112131.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Angiogenesis precedes myogenesis during regeneration following biopsy injury of skeletal muscle
    Authors: NL Jacobsen, AB Morton, SS Segal
    Skeletal Muscle, 2023-02-14;13(1):3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury
    Authors: ME Mohr, S Li, AM Trouten, RA Stairley, PL Roddy, C Liu, M Zhang, HM Sucov, G Tao
    bioRxiv : the preprint server for biology, 2023-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress
    Authors: Z Li, K Chen, Q Shao, H Lu, X Zhang, Y Pu, X Sun, H He, L Cao
    Journal of Neuroinflammation, 2023-01-30;20(1):16.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  24. Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization
    Authors: CA Pagani, AC Bancroft, RJ Tower, N Livingston, Y Sun, JY Hong, RN Kent, AL Strong, JH Nunez, JMR Medrano, N Patel, BA Nanes, KM Dean, Z Li, C Ge, BM Baker, AW James, SJ Weiss, RT Franceschi, B Levi
    Science Advances, 2022-12-21;8(51):eabq6152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  25. Kir4.1 channel activation in NG2 glia contributes to remyelination in ischemic stroke
    Authors: X Hong, Y Jian, S Ding, J Zhou, X Zheng, H Zhang, B Zhou, C Zhuang, J Wan, X Tong
    EBioMedicine, 2022-12-15;87(0):104406.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Effects of MgSO4 Alone or Associated with 4-PBA on Behavior and White Matter Integrity in a Mouse Model of Cerebral Palsy: A Sex- and Time-Dependent Study
    Authors: L Legouez, B Le Dieu-Lu, S Feillet, G Riou, M Yeddou, T Plouchart, N Dourmap, MA Le Ray, S Marret, BJ Gonzalez, C Cleren
    International Journal of Molecular Sciences, 2022-12-15;23(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. DOT1L regulates chamber-specific transcriptional networks during cardiogenesis and mediates postnatal cell cycle withdrawal
    Authors: P Cattaneo, MGB Hayes, N Baumgarten, D Hecker, S Peruzzo, GS Aslan, P Kunderfran, V Larcher, L Zhang, R Contu, G Fonseca, S Spinozzi, J Chen, G Condorelli, S Dimmeler, MH Schulz, S Heinz, N Guimarães-, SM Evans
    Nature Communications, 2022-12-02;13(1):7444.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  28. Ultrastructural characteristics of oligodendrocyte precursor cells in the early postnatal mouse optic nerve observed by serial block-face scanning electron microscopy
    Authors: K Ono, H Gotoh, T Nomura, T Morita, O Baba, M Matsumoto, S Saitoh, N Ohno
    PLoS ONE, 2022-12-01;17(12):e0278118.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. KLF9 and KLF13 transcription factors boost myelin gene expression in oligodendrocytes as partners of SOX10 and MYRF
    Authors: C Bernhardt, E Sock, F Fröb, S Hillgärtne, M Nemer, M Wegner
    Nucleic Acids Research, 2022-11-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
    Authors: T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons
    Nature Neuroscience, 2022-10-24;25(11):1446-1457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Vaccination-based immunotherapy to target profibrotic cells in liver and lung
    Authors: M Sobecki, J Chen, E Krzywinska, S Nagarajan, Z Fan, E Nelius, JM Monné Rodr, F Seehusen, A Hussein, G Moschini, EY Hajam, R Kiran, D Gotthardt, J Debbache, C Badoual, T Sato, T Isagawa, N Takeda, C Tanchot, E Tartour, A Weber, S Werner, J Loffing, L Sommer, V Sexl, C Münz, C Feghali-Bo, E Pachera, O Distler, J Snedeker, C Jamora, C Stockmann
    Cell Stem Cell, 2022-09-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  32. Evidence for oligodendrocyte progenitor cell heterogeneity in the adult mouse brain
    Authors: RM Beiter, C Rivet-Noor, AR Merchak, R Bai, DM Johanson, E Slogar, K Sol-Church, CC Overall, A Gaultier
    Scientific Reports, 2022-07-28;12(1):12921.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Transcriptome profiling of subepithelial PDGFRalpha cells in colonic mucosa reveals several cell-selective markers
    Authors: SE Ha, B Jin, BG Jorgensen, H Zogg, L Wei, R Singh, C Park, M Kurahashi, S Kim, G Baek, SM Poudrier, MY Lee, KM Sanders, S Ro
    PLoS ONE, 2022-05-13;17(5):e0261743.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. PDGFRalpha-induced stromal maturation is required to restrain postnatal intestinal epithelial stemness and promote defense mechanisms
    Authors: JM Jacob, SE Di Carlo, I Stzepourgi, A Lepelletie, PD Ndiaye, H Varet, R Legendre, E Kornobis, A Benabid, G Nigro, L Peduto
    Cell Stem Cell, 2022-05-05;29(5):856-868.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination
    Authors: S Ghorbani, E Jelinek, R Jain, B Buehner, C Li, BM Lozinski, S Sarkar, DK Kaushik, Y Dong, TN Wight, S Karimi-Abd, GJ Schenk, EM Strijbis, J Geurts, P Zhang, CC Ling, VW Yong
    Nature Communications, 2022-05-04;13(1):2445.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Maladaptive myelination promotes generalized epilepsy progression
    Authors: JK Knowles, H Xu, C Soane, A Batra, T Saucedo, E Frost, LT Tam, D Fraga, L Ni, K Villar, S Talmi, JR Huguenard, M Monje
    Nature Neuroscience, 2022-05-02;25(5):596-606.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  37. An estrogen-sensitive fibroblast population drives abdominal muscle fibrosis in an inguinal hernia mouse model
    Authors: T Potluri, MJ Taylor, JJ Stulberg, RL Lieber, H Zhao, SE Bulun
    JCI Insight, 2022-04-19;7(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfbeta Signaling in the Tumor Microenvironment
    Authors: Y Zhang, E Manouchehr, M Herre, J Cedervall, Q Qiao, Z Miao, A Hamidi, L Hellman, M Kamali-Mog, AK Olsson
    Cancers, 2022-04-12;14(8):.
    Species: Mouse
    Sample Types: Whole Cells
  39. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation
    Authors: S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli
    Journal of Cell Science, 2022-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  40. Restoring nuclear entry of Sirtuin 2 in oligodendrocyte progenitor cells promotes remyelination during ageing
    Authors: XR Ma, X Zhu, Y Xiao, HM Gu, SS Zheng, L Li, F Wang, ZJ Dong, DX Wang, Y Wu, C Yang, W Jiang, K Yao, Y Yin, Y Zhang, C Peng, L Gao, Z Meng, Z Hu, C Liu, L Li, HZ Chen, Y Shu, Z Ju, JW Zhao
    Nature Communications, 2022-03-09;13(1):1225.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  41. Mild respiratory SARS-CoV-2 infection can cause multi-lineage cellular dysregulation and myelin loss in the brain
    Authors: A Fernández-, P Lu, AC Geraghty, E Song, MH Lee, J Wood, B Yalç?n, KR Taylor, S Dutton, L Acosta-Alv, L Ni, D Contreras-, JR Gehlhausen, J Klein, C Lucas, T Mao, J Silva, MA Peña-Herná, A Tabachniko, T Takahashi, L Tabacof, J Tosto-Manc, E Breyman, A Kontorovic, D McCarthy, M Quezado, M Hefti, D Perl, R Folkerth, D Putrino, A Nath, A Iwasaki, M Monje
    bioRxiv : the preprint server for biology, 2022-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Involvement of PAR2 in platelet-derived growth factor receptor-alpha-positive cell proliferation in the colon of diabetic mice
    Authors: YJ Li, JP Ao, X Huang, HL Lu, HY Fu, NN Song, WX Xu, J Chen
    Physiological Reports, 2021-11-01;9(21):e15099.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Altered Mucus Barrier Integrity and Increased Susceptibility to Colitis in Mice upon Loss of Telocyte Bone Morphogenetic Protein Signalling
    Authors: V Reyes Nico, JM Allaire, AB Alfonso, D Pupo Gómez, V Pomerleau, V Giroux, F Boudreau, N Perreault
    Cells, 2021-10-29;10(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. Regulation of intestinal immunity and tissue repair by enteric glia
    Authors: F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis
    Nature, 2021-10-20;599(7883):125-130.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  45. Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs
    Authors: N Cohen, H Massalha, S Ben-Moshe, A Egozi, M Rozenberg, K Bahar Halp, S Itzkovitz
    PloS Biology, 2021-10-11;19(10):e3001214.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  46. PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination
    Authors: Y Wang, Y Zhang, S Zhang, B Kim, VL Hull, J Xu, P Prabhu, M Gregory, V Martinez-C, X Zhan, W Deng, F Guo
    Cell Reports, 2021-10-05;37(1):109695.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. NG2 glia-derived GABA release tunes inhibitory synapses and contributes to stress-induced anxiety
    Authors: X Zhang, Y Liu, X Hong, X Li, CK Meshul, C Moore, Y Yang, Y Han, WG Li, X Qi, H Lou, S Duan, TL Xu, X Tong
    Nature Communications, 2021-09-30;12(1):5740.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Sensitive timing of undifferentiation in oligodendrocyte progenitor cells and their enhanced maturation in primary visual cortex of binocularly enucleated mice
    Authors: H Shin, HD Kawai
    PLoS ONE, 2021-09-17;16(9):e0257395.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. Microglial ASD-related genes are involved in oligodendrocyte differentiation
    Authors: Y Takanezawa, S Tanabe, D Kato, R Ozeki, M Komoda, T Suzuki, H Baba, R Muramatsu
    Scientific Reports, 2021-09-08;11(1):17825.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  50. Molecular and functional characterization of detrusor PDGFRalpha positive cells in spinal cord injury-induced detrusor overactivity
    Authors: K Lee, SO Park, PC Choi, SB Ryoo, H Lee, LE Peri, T Zhou, RD Corrigan, AC Yanez, SB Moon, BA Perrino, KM Sanders, SD Koh
    Scientific Reports, 2021-08-11;11(1):16268.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  51. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation
    Authors: L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch
    Cell Stem Cell, 2021-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages
    Authors: I De, V Maklakova, S Litscher, MM Boyd, LC Klemm, Z Wang, C Kendziorsk, LS Collier
    Journal of Neuroinflammation, 2021-07-19;18(1):162.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  53. Regional specialization and fate specification of bone stromal cells in skeletal development
    Authors: KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams
    Cell Reports, 2021-07-13;36(2):109352.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  54. Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality
    Authors: SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza
    Cell Reports, 2021-06-08;35(10):109223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  55. Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle
    Authors: A Waisman, AM Norris, M Elías Cost, D Kopinke
    Scientific Reports, 2021-06-03;11(1):11793.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  56. NF1 mutation drives neuronal�activity-dependent initiation of optic glioma
    Authors: Y Pan, JD Hysinger, T Barron, NF Schindler, O Cobb, X Guo, B Yalç?n, C Anastasaki, SB Mulinyawe, A Ponnuswami, S Scheaffer, Y Ma, KC Chang, X Xia, JA Toonen, JJ Lennon, EM Gibson, JR Huguenard, LM Liau, JL Goldberg, M Monje, DH Gutmann
    Nature, 2021-05-26;594(7862):277-282.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  57. The Impact of Fixation on the Detection of Oligodendrocyte Precursor Cell Morphology and Vascular Associations
    Authors: F Pfeiffer, A Sherafat, A Nishiyama
    Cells, 2021-05-24;10(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  58. PDGF-PDGFR network differentially regulates the fate, migration, proliferation, and cell cycle progression of myogenic cells
    Authors: O Contreras, A Córdova-Ca, E Brandan
    Cellular Signalling, 2021-05-08;84(0):110036.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC/IF, Western Blot
  59. Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells
    Authors: MN Wosczyna, EE Perez Carb, MW Wagner, S Paredes, CT Konishi, L Liu, TT Wang, RA Walsh, Q Gan, CS Morrissey, TA Rando
    Cell Stem Cell, 2021-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  60. Contribution of PDGFR&alpha-positive cells in maintenance and injury responses in mouse large vessels
    Authors: K Kimura, K Ramirez, TAV Nguyen, Y Yamashiro, A Sada, H Yanagisawa
    Scientific Reports, 2021-04-21;11(1):8683.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  61. Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice
    Authors: W Mu, S Li, J Xu, X Guo, H Wu, Z Chen, L Qiao, G Helfer, F Lu, C Liu, QF Wu
    Nature Communications, 2021-04-16;12(1):2288.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophy
    Authors: L Mészáros, MJ Riemenschn, H Gassner, F Marxreiter, S von Hörste, A Hoffmann, J Winkler
    Acta neuropathologica communications, 2021-04-14;9(1):68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  63. Identification of PDGFR&alpha-positive interstitial cells in the distal segment of the murine vas deferens
    Authors: T Hiroshige, KI Uemura, S Hirashima, K Hino, A Togo, K Ohta, T Igawa, KI Nakamura
    Scientific Reports, 2021-04-06;11(1):7553.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  64. Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
    Authors: S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu
    Nature, 2021-03-31;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  65. PDGFR&alpha: Expression and Function during Mitral Valve Morphogenesis
    Authors: K Moore, D Fulmer, L Guo, N Koren, J Glover, R Moore, C Gensemer, T Beck, J Morningsta, R Stairley, RA Norris
    Journal of cardiovascular development and disease, 2021-03-13;8(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  66. Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis
    Authors: D Yamakawa, D Katoh, K Kasahara, T Shiromizu, M Matsuyama, C Matsuda, Y Maeno, M Watanabe, Y Nishimura, M Inagaki
    Cell Reports, 2021-03-09;34(10):108817.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  67. Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2
    Authors: LL Boshans, H Soh, WM Wood, TM Nolan, II Mandoiu, Y Yanagawa, AV Tzingounis, A Nishiyama
    Scientific Reports, 2021-02-11;11(1):3552.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  68. SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors
    Authors: G Giuliani, S Vumbaca, C Fuoco, C Gargioli, E Giorda, G Massacci, A Palma, A Reggio, F Riccio, M Rosina, M Vinci, L Castagnoli, G Cesareni
    Cell Death & Disease, 2021-01-25;12(1):122.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  69. Periaxonal and nodal plasticities modulate action potential conduction in the adult mouse brain
    Authors: CL Cullen, RE Pepper, MT Clutterbuc, KA Pitman, V Oorschot, L Auderset, AD Tang, G Ramm, B Emery, J Rodger, RB Jolivet, KM Young
    Cell Reports, 2021-01-19;34(3):108641.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  70. Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis
    Authors: MD Brügger, T Valenta, H Fazilaty, G Hausmann, K Basler
    PloS Biology, 2020-12-11;18(12):e3001032.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  71. Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury
    Authors: EM Floriddia, T Lourenço, S Zhang, D van Brugge, MM Hilscher, P Kukanja, JP Gonçalves, M Alt?nkök, C Yokota, E Llorens-Bo, SB Mulinyawe, M Grãos, LO Sun, J Frisén, M Nilsson, G Castelo-Br
    Nat Commun, 2020-11-17;11(1):5860.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  72. Neuronal activity-dependent myelin repair promotes motor function recovery after contusion spinal cord injury
    Authors: M Luo, Y Yin, D Li, W Tang, Y Liu, L Pan, L Yu, B Tan
    Brain Res Bull, 2020-11-14;166(0):73-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  73. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin
    Authors: D Jiang, S Christ, D Correa-Gal, P Ramesh, S Kalgudde G, J Wannemache, CH Mayr, V Lupperger, Q Yu, H Ye, M Mück-Häusl, V Rajendran, L Wan, J Liu, U Mirastschi, T Volz, C Marr, HB Schiller, Y Rinkevich
    Nat Commun, 2020-11-06;11(1):5653.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  74. PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease
    Authors: K Song, Y Qing, Q Guo, EK Peden, C Chen, WE Mitch, L Truong, J Cheng
    JCI Insight, 2020-11-05;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  75. Retinoic acid signalling in fibro/adipogenic progenitors robustly enhances muscle regeneration
    Authors: L Zhao, JS Son, B Wang, Q Tian, Y Chen, X Liu, JM de Avila, MJ Zhu, M Du
    EBioMedicine, 2020-09-24;60(0):103020.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  76. Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi
    Authors: SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh
    Nat Commun, 2020-08-14;11(1):4102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  77. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  78. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  79. BCAS1-positive immature oligodendrocytes are affected by the &alpha-synuclein-induced pathology of multiple system atrophy
    Authors: S Kaji, T Maki, J Ueda, T Ishimoto, Y Inoue, K Yasuda, M Sawamura, R Hikawa, T Ayaki, H Yamakado, R Takahashi
    Acta Neuropathol Commun, 2020-07-29;8(1):120.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  80. The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination
    Authors: A Pu, MK Mishra, Y Dong, S Ghorbaniga, EL Stephenson, KS Rawji, C Silva, H Kitagawa, S Sawcer, VW Yong
    J Neuroinflammation, 2020-07-23;17(1):220.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  81. TGF-beta-driven downregulation of the transcription factor TCF7L2 affects Wnt/beta-catenin signaling in PDGFRalpha+ fibroblasts
    Authors: O Contreras, H Soliman, M Theret, FMV Rossi, E Brandan
    J. Cell. Sci., 2020-06-19;133(12):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  82. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth
    Authors: S Davidson, M Efremova, A Riedel, B Mahata, J Pramanik, J Huuhtanen, G Kar, R Vento-Torm, T Hagai, X Chen, MA Haniffa, JD Shields, SA Teichmann
    Cell Rep, 2020-05-19;31(7):107628.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  83. Embryonic microglia influence developing hypothalamic glial populations
    Authors: CM Marsters, D Nesan, R Far, N Klenin, QJ Pittman, DM Kurrasch
    J Neuroinflammation, 2020-05-06;17(1):146.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  84. Interstitial Cell Remodeling Promotes Aberrant Adipogenesis in Dystrophic Muscles
    Authors: J Camps, N Breuls, A Sifrim, N Giarratana, M Corvelyn, L Danti, H Grosemans, S Vanuytven, I Thiry, M Belicchi, M Meregalli, K Platko, ME MacDonald, RC Austin, R Gijsbers, G Cossu, Y Torrente, T Voet, M Sampaolesi
    Cell Rep, 2020-05-05;31(5):107597.
    Species: Human
    Sample Types: Whole Cells
  85. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Human
    Sample Types:
    Applications: IF
  86. Lgr5+�telocytes are a signaling source at the intestinal villus tip
    Authors: K Bahar Halp, H Massalha, RK Zwick, AE Moor, D Castillo-A, M Rozenberg, L Farack, A Egozi, DR Miller, I Averbukh, Y Harnik, N Weinberg-C, FJ de Sauvage, I Amit, OD Klein, M Shoshkes-C, S Itzkovitz
    Nat Commun, 2020-04-22;11(1):1936.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  87. Distinct Mesenchymal Cell Populations Generate the Essential Intestinal BMP Signaling Gradient
    Authors: N McCarthy, E Manieri, EE Storm, A Saadatpour, AM Luoma, VN Kapoor, S Madha, LT Gaynor, C Cox, S Keerthivas, K Wucherpfen, GC Yuan, FJ de Sauvage, SJ Turley, RA Shivdasani
    Cell Stem Cell, 2020-02-20;0(0):.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  88. TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung
    Authors: I Wojahn, TH Lüdtke, VM Christoffe, MO Trowe, A Kispert
    Respir. Res., 2019-12-23;20(1):292.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  89. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation
    Authors: A Grund, M Szaroszyk, M Korf-Kling, M Malek Moha, FA Trogisch, U Schrameck, A Gigina, C Tiedje, M Gaestel, T Kraft, J Hegermann, S Batkai, T Thum, A Perrot, CD Remedios, E Riechert, M Völkers, S Doroudgar, A Jungmann, R Bauer, X Yin, M Mayr, KC Wollert, A Pich, H Xiao, HA Katus, J Bauersachs, OJ Müller, J Heineke
    EMBO Mol Med, 2019-08-30;0(0):e10018.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  90. Functional Inactivation of Mast Cells Enhances Subcutaneous Adipose Tissue Browning in Mice
    Authors: X Zhang, X Wang, H Yin, L Zhang, A Feng, QX Zhang, Y Lin, B Bao, LL Hernandez, GP Shi, J Liu
    Cell Rep, 2019-07-16;28(3):792-803.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  91. Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy
    Authors: SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball
    Nat Commun, 2019-06-26;10(1):2788.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  92. Fibroadipogenic progenitors are responsible for muscle loss in limb girdle muscular dystrophy 2B
    Authors: MW Hogarth, A Defour, C Lazarski, E Gallardo, JD Manera, TA Partridge, K Nagaraju, JK Jaiswal
    Nat Commun, 2019-06-03;10(1):2430.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  93. Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle
    Authors: MN Wosczyna, CT Konishi, EE Perez Carb, TT Wang, RA Walsh, Q Gan, MW Wagner, TA Rando
    Cell Rep, 2019-05-14;27(7):2029-2035.e5.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC frozen fixed
  94. In Situ Modification of Tissue Stem and Progenitor Cell Genomes
    Authors: JM Goldstein, M Tabebordba, K Zhu, LD Wang, KA Messemer, B Peacker, S Ashrafi Ka, M Gonzalez-C, Y Shwartz, JKW Cheng, R Xiao, T Barungi, C Albright, YC Hsu, LH Vandenberg, AJ Wagers
    Cell Rep, 2019-04-23;27(4):1254-1264.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  95. Myelin repair stimulated by CNS-selective thyroid hormone action
    Authors: MD Hartley, T Banerji, IJ Tagge, LL Kirkemo, P Chaudhary, E Calkins, D Galipeau, MD Shokat, MJ DeBell, S Van Leuven, H Miller, G Marracci, E Pocius, T Banerji, SJ Ferrara, JM Meinig, B Emery, D Bourdette, TS Scanlan
    JCI Insight, 2019-04-18;4(8):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  96. PDGFR?+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland
    Authors: PA Joshi, PD Waterhouse, K Kasaian, H Fang, O Gulyaeva, HS Sul, PC Boutros, R Khokha
    Nat Commun, 2019-04-15;10(1):1760.
    Species: Mouse
    Sample Types: Whole Tissues
    Applications: IHC
  97. Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration
    Authors: X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou
    Stem Cell Reports, 2019-02-14;12(3):624-638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  98. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
    Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
    Nat Commun, 2019-02-08;10(1):650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  99. Aging Disrupts Muscle Stem Cell Function by Impairing Matricellular WISP1 Secretion from Fibro-Adipogenic Progenitors
    Authors: L Lukjanenko, S Karaz, P Stuelsatz, U Gurriaran-, J Michaud, G Dammone, F Sizzano, O Mashinchia, S Ancel, E Migliavacc, S Liot, G Jacot, S Metairon, F Raymond, P Descombes, A Palini, B Chazaud, MA Rudnicki, CF Bentzinger, JN Feige
    Cell Stem Cell, 2019-01-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  100. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage
    Authors: D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar
    Nat. Immunol., 2019-01-14;20(2):163-172.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  101. Profiling proliferative cells and their progeny in damaged murine hearts
    Authors: K Kretzschma, Y Post, M Bannier-Hé, A Mattiotti, J Drost, O Basak, VSW Li, M van den Bo, QD Gunst, D Versteeg, L Kooijman, S van der El, JH van Es, E van Rooij, MJB van den Ho, H Clevers
    Proc. Natl. Acad. Sci. U.S.A., 2018-12-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  102. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
    Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
    Cell Stem Cell, 2018-11-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  103. Heterozygosity for Nuclear Factor One X in mice models features of Malan syndrome
    Authors: S Oishi, D Harkins, ND Kurniawan, M Kasherman, L Harris, O Zalucki, RM Gronostajs, THJ Burne, M Piper
    EBioMedicine, 2018-11-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  104. AMPK Activation Regulates LTBP4-Dependent TGF-?1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy
    Authors: G Juban, M Saclier, H Yacoub-You, A Kernou, L Arnold, C Boisson, S Ben Larbi, M Magnan, S Cuvellier, M Théret, BJ Petrof, I Desguerre, J Gondin, R Mounier, B Chazaud
    Cell Rep, 2018-11-20;25(8):2163-2176.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  105. Multimodal Enhancement of Remyelination by Exercise with a Pivotal Role for Oligodendroglial PGC1?
    Authors: SK Jensen, NJ Michaels, S Ilyntskyy, MB Keough, O Kovalchuk, VW Yong
    Cell Rep, 2018-09-18;24(12):3167-3179.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  106. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018-06-22;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  107. Perfect chronic skeletal muscle regeneration in adult spiny mice, Acomys cahirinus
    Authors: M Maden, JO Brant, A Rubiano, AGW Sandoval, C Simmons, R Mitchell, H Collin-Hoo, J Jacobson, S Omairi, K Patel
    Sci Rep, 2018-06-11;8(1):8920.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  108. Anti-inflammatory role of 15-lipoxygenase contributes to the maintenance of skin integrity in mice
    Authors: SN Kim, S Akindehin, HJ Kwon, YH Son, A Saha, YS Jung, JK Seong, KM Lim, JH Sung, KR Maddipati, YH Lee
    Sci Rep, 2018-06-11;8(1):8856.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  109. Myelination of the developing lateral olfactory tract and anterior commissure
    Authors: LN Collins, DL Hill, PC Brunjes
    J. Comp. Neurol., 2018-04-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  110. B-1a lymphocytes promote oligodendrogenesis during brain development
    Authors: S Tanabe, T Yamashita
    Nat. Neurosci., 2018-03-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  111. Role of P2XReceptor in Mouse Voiding Function
    Authors: W Yu, WG Hill, SC Robson, ML Zeidel
    Sci Rep, 2018-01-30;8(1):1838.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  112. Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner
    Authors: S Mitew, I Gobius, LR Fenlon, SJ McDougall, D Hawkes, YL Xing, H Bujalka, AL Gundlach, LJ Richards, TJ Kilpatrick, TD Merson, B Emery
    Nat Commun, 2018-01-22;9(1):306.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  113. Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion
    Authors: S Begolly, JA Olschowka, T Love, JP Williams, MK O'Banion
    Glia, 2017-12-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  114. Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
    Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
    PLoS ONE, 2017-12-18;12(12):e0189380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  115. Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice
    Authors: H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu
    Cell. Physiol. Biochem., 2017-08-28;43(1):158-171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  116. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017-08-14;12(8):e0182265.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  117. Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries
    Authors: H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain
    Dis Model Mech, 2017-07-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  118. ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers
    Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan
    PLoS ONE, 2017-05-16;12(5):e0177649.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  119. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
    Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
    JCI Insight, 2017-03-23;2(6):e89206.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  120. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
    Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
    Cell Stem Cell, 2017-01-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  121. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017-01-16;7(0):40397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  122. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016-12-19;6(0):39457.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  123. Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation
    Cell Rep, 2016-11-22;17(9):2312-2325.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  124. Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor
    Authors: Dong-An Wang
    Acta Biomater, 2016-11-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  125. USP9X deletion elevates the density of oligodendrocytes within the postnatal dentate gyrus
    Authors: Michael Piper
    Neurogenesis (Austin), 2016-09-30;3(1):e1235524.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  126. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development
    Authors: K Noda, M Kitami, K Kitami, M Kaku, Y Komatsu
    Proc Natl Acad Sci USA, 2016-04-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  127. Spatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model
    Authors: I Tagge, A O'Connor, P Chaudhary, J Pollaro, Y Berlow, M Chalupsky, D Bourdette, R Woltjer, M Johnson, W Rooney
    PLoS ONE, 2016-04-07;11(4):e0152480.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  128. AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to ?-adrenergic signalling
    Authors: JH Shin, SH Lee, YN Kim, IY Kim, YJ Kim, DS Kyeong, HJ Lim, SY Cho, J Choi, YJ Wi, JH Choi, YS Yoon, YS Bae, JK Seong
    Sci Rep, 2016-03-18;6(0):23426.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  129. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model.
    Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R
    Sci Rep, 2015-02-16;5(0):8468.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  130. Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft.
    Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y
    J Immunol, 2015-01-19;194(4):1996-2003.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  131. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2014-10-24;22(2):351-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  132. Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
    Authors: Pinto, Mauricio, Dye, Wendy W, Jacobsen, Britta M, Horwitz, Kathryn
    BMC Cancer, 2014-10-01;14(0):735.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  133. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.
    Authors: Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, Masliah E, Winner B, Wegner M, Winkler J
    Mol Cell Neurosci, 2014-07-11;62(0):68-78.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  134. Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis.
    Authors: Moore-Morris T, Guimaraes-Camboa N, Banerjee I, Zambon A, Kisseleva T, Velayoudon A, Stallcup W, Gu Y, Dalton N, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner D, Peterson K, Chen J, Evans S
    J Clin Invest, 2014-06-17;124(7):2921-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  135. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain.
    Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle
    Science, 2014-04-10;344(6183):1252304.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  136. Nitric oxide controls fat deposition in dystrophic skeletal muscle by regulating fibro-adipogenic precursor differentiation.
    Authors: Cordani N, Pisa V, Pozzi L, Sciorati C, Clementi E
    Stem Cells, 2014-04-01;32(4):874-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  137. Hypoxia inducible factor-2alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors.
    Authors: Duan L, Takeda K, Fong G
    PLoS ONE, 2014-01-27;9(1):e84736.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  138. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2013-12-18;141(2):448-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  139. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  140. Abnormal development of NG2+PDGFR-alpha+ neural progenitor cells leads to neonatal hydrocephalus in a ciliopathy mouse model.
    Authors: Carter C, Vogel T, Zhang Q, Seo S, Swiderski R, Moninger T, Cassell M, Thedens D, Keppler-Noreuil K, Nopoulos P, Nishimura D, Searby C, Bugge K, Sheffield V
    Nat Med, 2012-11-18;18(12):1797-804.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  141. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010-03-15;207(4):867-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  142. Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke.
    Authors: Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA
    Nat. Med., 2008-06-22;14(7):731-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  143. Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis.
    Authors: Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S
    J. Neuroimmunol., 2008-05-08;196(1):67-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  144. Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis.
    Authors: Luyt K, Varadi A, Durant CF, Molnar E
    J. Neurochem., 2006-07-06;99(2):641-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  145. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006-05-01;12(9):2676-88.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

  1. Does Mouse PDGF R alpha Antibody, Catalog # AF1062, cross-react with rat PDGF R alpha?

    • We have not tested Catalog # AF1062 against rat samples in-house so we do not guarantee that it will detect rat PDGF R alpha. However, Uniprot indicates the homology between mouse and rat PDGF R to be 95.7%, which suggests cross-reactivity is likely. 

View all Antibody FAQs
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Reviews for Mouse PDGF R alpha Antibody

Average Rating: 4.5 (Based on 12 Reviews)

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Mouse PDGF R alpha Antibody
By Anonymous on 07/14/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: fixed mouse brain Species: Mouse

1:100, free-floating


Mouse PDGF R alpha Antibody
By Anonymous on 10/20/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult pancreas Species: Mouse

Primary goat anti-mouse PDGFRa (1:300) with Donkey anti-goat AF647 (1:500) on adult mouse pancreas. It works well and clearly shows specific staining of fibroblasts.


Mouse PDGF R alpha Antibody
By Anonymous on 06/28/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain stem tissue Species: Mouse and Mouse Embryo

Mouse PDGF R alpha Antibody
By Anonymous on 05/10/2020
Application: WB Sample Tested: Adult heart Species: Mouse

3 mice hearts extractions after MI.
HSP60 was used as loading control.
There is a non specific band around 80 kDa.


Mouse PDGF R alpha Antibody
By Anonymous on 05/10/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: primary cardiac fibroblasts Species: Rat

rat primary cardiac fibroblasts, PDGFRa in red, DAPI in blue


Mouse PDGF R alpha Antibody
By Anonymous on 05/10/2020
Application: IHC Sample Tested: Adult heart Species: Mouse

mouse heart post MI, troponin I in green, PDGFRa in red, DAPI in blue


Mouse PDGF R alpha Antibody
By Anonymous on 10/20/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse

Dilution 1:200


Mouse PDGF R alpha Antibody
By Jun Cai on 01/16/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Spinal cord tissue Species: Mouse

Mouse PDGF R alpha Antibody
By Thiruloga Chandru on 07/27/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Mouse PDGF R alpha Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Mouse PDGF R alpha Antibody
By Anonymous on 01/13/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryo Species: Mouse

differentiation assay using e12.5 mouse neural stem cells.
PDGFR (red)/ Dapi (blue)


Mouse PDGF R alpha Antibody
By Anonymous on 08/10/2017
Application: IHC Sample Tested: Brain (cerebellum) tissue Species: Mouse