hh Mouse PDGF R alpha Antibody AF1062: R&D Systems

Mouse PDGF R alpha Antibody

(29 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
    Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
    Accession # P26618
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse PDGF R alpha by Western Blot. Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

  • References:
    1. Betsholtz, C. et al. (2001) BioEssays 23:494.
    2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
    3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
    4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  • Long Name:
    Platelet-derived Growth Factor Receptor alpha
  • Entrez Gene IDs:
    5156 (Human); 18595 (Mouse)
  • Alternate Names:
    alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries
    Authors: H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers
    Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan
    PLoS ONE, 2017;12(5):e0177649.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017;12(8):e0182265.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice
    Authors: H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu
    Cell. Physiol. Biochem., 2017;43(1):158-171.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
    Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
    JCI Insight, 2017;2(6):e89206.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017;7(0):40397.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  7. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
    Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  8. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development
    Authors: K Noda, M Kitami, K Kitami, M Kaku, Y Komatsu
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to ?-adrenergic signalling
    Authors: JH Shin, SH Lee, YN Kim, IY Kim, YJ Kim, DS Kyeong, HJ Lim, SY Cho, J Choi, YJ Wi, JH Choi, YS Yoon, YS Bae, JK Seong
    Sci Rep, 2016;6(0):23426.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  10. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016;6(0):39457.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Spatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model
    Authors: I Tagge, A O'Connor, P Chaudhary, J Pollaro, Y Berlow, M Chalupsky, D Bourdette, R Woltjer, M Johnson, W Rooney
    PLoS ONE, 2016;11(4):e0152480.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation
    Cell Rep, 2016;17(9):2312-2325.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  13. Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft.
    Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y
    J Immunol, 2015;194(4):1996-2003.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  14. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2015;22(2):351-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  15. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model.
    Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R
    Sci Rep, 2015;5(0):8468.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  16. Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis.
    Authors: Moore-Morris T, Guimaraes-Camboa N, Banerjee I, Zambon A, Kisseleva T, Velayoudon A, Stallcup W, Gu Y, Dalton N, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner D, Peterson K, Chen J, Evans S
    J Clin Invest, 2014;124(7):2921-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  17. Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
    Authors: Pinto, Mauricio, Dye, Wendy W, Jacobsen, Britta M, Horwitz, Kathryn
    BMC Cancer, 2014;14(0):735.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fluorescence
  18. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain.
    Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle
    Science, 2014;344(6183):1252304.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  19. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.
    Authors: Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, Masliah E, Winner B, Wegner M, Winkler J
    Mol Cell Neurosci, 2014;62(0):68-78.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  20. Nitric oxide controls fat deposition in dystrophic skeletal muscle by regulating fibro-adipogenic precursor differentiation.
    Authors: Cordani N, Pisa V, Pozzi L, Sciorati C, Clementi E
    Stem Cells, 2014;32(4):874-85.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2014;141(2):448-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  22. Hypoxia inducible factor-2alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors.
    Authors: Duan L, Takeda K, Fong G
    PLoS ONE, 2014;9(1):e84736.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  23. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  24. Abnormal development of NG2+PDGFR-alpha+ neural progenitor cells leads to neonatal hydrocephalus in a ciliopathy mouse model.
    Authors: Carter C, Vogel T, Zhang Q, Seo S, Swiderski R, Moninger T, Cassell M, Thedens D, Keppler-Noreuil K, Nopoulos P, Nishimura D, Searby C, Bugge K, Sheffield V
    Nat Med, 2012;18(12):1797-804.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  25. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  26. Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis.
    Authors: Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S
    J. Neuroimmunol., 2008;196(1):67-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  27. Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke.
    Authors: Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA
    Nat. Med., 2008;14(7):731-7.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  28. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006;12(9):2676-88.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  29. Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis.
    Authors: Luyt K, Varadi A, Durant CF, Molnar E
    J. Neurochem., 2006;99(2):641-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 29 Citations
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationImmunohistochemistry
Sample TestedBrain (cerebellum) tissue
SpeciesMouse

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