Mouse PDGF R alpha Antibody

Name: Mouse PDGF R alpha Antibody
Brand: R&D Systems
Rating: 4 (2 reviews)

(35 citations)

(2 Reviews)

Datasheet / CoA / SDS

Western Blot

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Immunohistochemistry

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Immunohistochemistry

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Neutralization

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Product Details

Citations (35)

FAQs

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Species Reactivity

Mouse
Specificity

Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

See below
Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Mouse PDGF R alpha by Western Blot. Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry click image to view larger	PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry click image to view larger	PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization click image to view larger	Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND₅₀ is typically 1-5 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta bind PDGF with high affinity and are potent PDGF antagonists.

References:
1. Betsholtz, C. et al. (2001) BioEssays 23:494.
2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Long Name:

Platelet-derived Growth Factor Receptor alpha

Entrez Gene IDs:

5156 (Human); 18595 (Mouse)

Alternate Names:

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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Species	Applications	Sample Type
B-1a lymphocytes promote oligodendrogenesis during brain development Authors: S Tanabe, T Yamashita Nat. Neurosci., 2018;0(0):. Species: Mouse Sample Type: Whole Cells Application: ICC Role of P2XReceptor in Mouse Voiding Function Authors: W Yu, WG Hill, SC Robson, ML Zeidel Sci Rep, 2018;8(1):1838. Species: Mouse Sample Type: Whole Tissue Application: IHC-Fr Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner Authors: S Mitew, I Gobius, LR Fenlon, SJ McDougall, D Hawkes, YL Xing, H Bujalka, AL Gundlach, LJ Richards, TJ Kilpatrick, TD Merson, B Emery Nat Commun, 2018;9(1):306. Species: Mouse Sample Type: Whole Tissue Application: IHC Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang PLoS ONE, 2017;12(12):e0189380. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries Authors: H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain Dis Model Mech, 2017;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan PLoS ONE, 2017;12(5):e0177649. Species: Mouse Sample Type: Tissue Homogenates Application: WB Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro PLoS ONE, 2017;12(8):e0182265. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice Authors: H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu Cell. Physiol. Biochem., 2017;43(1):158-171. Species: Mouse Sample Type: Whole Tissue Application: IHC Physiologically activated mammary fibroblasts promote postpartum mammary cancer Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin JCI Insight, 2017;2(6):e89206. Species: Mouse Sample Type: Whole Tissue Application: IHC Leptin sustains spontaneous remyelination in the adult central nervous system Authors: K Matoba, R Muramatsu, T Yamashita Sci Rep, 2017;7(0):40397. Species: Mouse Sample Type: Whole Tissue Application: IHC - PFA fixed Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells Inï¿½Vivo Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans Cell Stem Cell, 2017;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen USP9X deletion elevates the density of oligodendrocytes within the postnatal dentate gyrus Authors: Michael Piper Neurogenesis (Austin), 2016;3(1):e1235524. Species: Mouse Sample Type: Whole Tissue Application: IHC Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos Sci Rep, 2016;6(0):39457. Species: Mouse Sample Type: Whole Cells Application: ICC Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor Authors: Dong-An Wang Acta Biomater, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: ICC AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to ?-adrenergic signalling Authors: JH Shin, SH Lee, YN Kim, IY Kim, YJ Kim, DS Kyeong, HJ Lim, SY Cho, J Choi, YJ Wi, JH Choi, YS Yoon, YS Bae, JK Seong Sci Rep, 2016;6(0):23426. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Spatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model Authors: I Tagge, A O'Connor, P Chaudhary, J Pollaro, Y Berlow, M Chalupsky, D Bourdette, R Woltjer, M Johnson, W Rooney PLoS ONE, 2016;11(4):e0152480. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development Authors: K Noda, M Kitami, K Kitami, M Kaku, Y Komatsu Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation Cell Rep, 2016;17(9):2312-2325. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft. Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y J Immunol, 2015;194(4):1996-2003. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model. Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R Sci Rep, 2015;5(0):8468. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development. Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G Cell Death Differ, 2015;22(2):351-63. Species: Mouse Sample Type: Whole Tissue Application: IF Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain. Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle Science, 2014;344(6183):1252304. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded Hypoxia inducible factor-2alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors. Authors: Duan L, Takeda K, Fong G PLoS ONE, 2014;9(1):e84736. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R Development, 2014;141(2):448-59. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells. Authors: Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, Masliah E, Winner B, Wegner M, Winkler J Mol Cell Neurosci, 2014;62(0):68-78. Species: Rat Sample Type: Whole Cells Application: ICC Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling. Authors: Pinto, Mauricio, Dye, Wendy W, Jacobsen, Britta M, Horwitz, Kathryn BMC Cancer, 2014;14(0):735. Species: Mouse Sample Type: Whole Cells Application: IHC Fluorescence Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis. Authors: Moore-Morris T, Guimaraes-Camboa N, Banerjee I, Zambon A, Kisseleva T, Velayoudon A, Stallcup W, Gu Y, Dalton N, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner D, Peterson K, Chen J, Evans S J Clin Invest, 2014;124(7):2921-34. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded Nitric oxide controls fat deposition in dystrophic skeletal muscle by regulating fibro-adipogenic precursor differentiation. Authors: Cordani N, Pisa V, Pozzi L, Sciorati C, Clementi E Stem Cells, 2014;32(4):874-85. Species: Mouse Sample Type: Whole Tissue Application: IHC Distinct fibroblast lineages determine dermal architecture in skin development and repair. Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Nature, 2013;504(7479):277-81. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Abnormal development of NG2+PDGFR-alpha+ neural progenitor cells leads to neonatal hydrocephalus in a ciliopathy mouse model. Authors: Carter C, Vogel T, Zhang Q, Seo S, Swiderski R, Moninger T, Cassell M, Thedens D, Keppler-Noreuil K, Nopoulos P, Nishimura D, Searby C, Bugge K, Sheffield V Nat Med, 2012;18(12):1797-804. Species: Mouse Sample Type: Tissue Homogenates Application: IP Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X J. Exp. Med., 2010;207(4):867-80. Species: Mouse Sample Type: In Vivo Application: In vivo Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis. Authors: Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S J. Neuroimmunol., 2008;196(1):67-81. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke. Authors: Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA Nat. Med., 2008;14(7):731-7. Species: Mouse Sample Type: Cell Lysates Application: WB Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N Clin. Cancer Res., 2006;12(9):2676-88. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis. Authors: Luyt K, Varadi A, Durant CF, Molnar E J. Neurochem., 2006;99(2):641-56. Species: Mouse Sample Type: Whole Cells Application: ICC Expand to show all Citations

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Mouse PDGF R alpha Antibody

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Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Basic

VisUCyte Antigen Retrieval Reagent-Universal

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Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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