Home / PDGF R alpha / Mouse PDGF R alpha Antibody

Mouse PDGF R alpha Antibody

AF1062 38 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1062
AF1062-SP
Detection of Mouse PDGF R alpha by Western Blot.
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Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PDGF R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse PDGF R alpha by Western Blot. View Larger

Detection of Mouse PDGF R alpha by Western Blot. Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PDGF R alpha in Mouse Embryo. View Larger

PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry PDGF R alpha in Mouse Embryo. View Larger

PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. View Larger

Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

References
  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Datasheets

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COA
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Citations for Mouse PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. B-1a lymphocytes promote oligodendrogenesis during brain development
    Authors: S Tanabe, T Yamashita
    Nat. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Anti-inflammatory role of 15-lipoxygenase contributes to the maintenance of skin integrity in mice
    Authors: SN Kim, S Akindehin, HJ Kwon, YH Son, A Saha, YS Jung, JK Seong, KM Lim, JH Sung, KR Maddipati, YH Lee
    Sci Rep, 2018;8(1):8856.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner
    Authors: S Mitew, I Gobius, LR Fenlon, SJ McDougall, D Hawkes, YL Xing, H Bujalka, AL Gundlach, LJ Richards, TJ Kilpatrick, TD Merson, B Emery
    Nat Commun, 2018;9(1):306.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Role of P2XReceptor in Mouse Voiding Function
    Authors: W Yu, WG Hill, SC Robson, ML Zeidel
    Sci Rep, 2018;8(1):1838.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Perfect chronic skeletal muscle regeneration in adult spiny mice, Acomys cahirinus
    Authors: M Maden, JO Brant, A Rubiano, AGW Sandoval, C Simmons, R Mitchell, H Collin-Hoo, J Jacobson, S Omairi, K Patel
    Sci Rep, 2018;8(1):8920.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017;7(0):40397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - PFA fixed
  8. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017;12(8):e0182265.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  9. Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice
    Authors: H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu
    Cell. Physiol. Biochem., 2017;43(1):158-171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
    Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
    JCI Insight, 2017;2(6):e89206.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries
    Authors: H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
    Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  13. ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers
    Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan
    PLoS ONE, 2017;12(5):e0177649.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  14. Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
    Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
    PLoS ONE, 2017;12(12):e0189380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  15. Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation
    Cell Rep, 2016;17(9):2312-2325.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  16. Real-time and Non-invasive Monitoring of Embryonic Stem Cell Survival during the Development of Embryoid Bodies with Smart Nanosensor
    Authors: Dong-An Wang
    Acta Biomater, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Spatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model
    Authors: I Tagge, A O'Connor, P Chaudhary, J Pollaro, Y Berlow, M Chalupsky, D Bourdette, R Woltjer, M Johnson, W Rooney
    PLoS ONE, 2016;11(4):e0152480.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  18. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016;6(0):39457.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to ?-adrenergic signalling
    Authors: JH Shin, SH Lee, YN Kim, IY Kim, YJ Kim, DS Kyeong, HJ Lim, SY Cho, J Choi, YJ Wi, JH Choi, YS Yoon, YS Bae, JK Seong
    Sci Rep, 2016;6(0):23426.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  20. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development
    Authors: K Noda, M Kitami, K Kitami, M Kaku, Y Komatsu
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  21. USP9X deletion elevates the density of oligodendrocytes within the postnatal dentate gyrus
    Authors: Michael Piper
    Neurogenesis (Austin), 2016;3(1):e1235524.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2015;22(2):351-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  23. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model.
    Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R
    Sci Rep, 2015;5(0):8468.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  24. Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft.
    Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y
    J Immunol, 2015;194(4):1996-2003.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  25. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain.
    Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle
    Science, 2014;344(6183):1252304.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  26. Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis.
    Authors: Moore-Morris T, Guimaraes-Camboa N, Banerjee I, Zambon A, Kisseleva T, Velayoudon A, Stallcup W, Gu Y, Dalton N, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner D, Peterson K, Chen J, Evans S
    J Clin Invest, 2014;124(7):2921-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  27. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2014;141(2):448-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  28. Nitric oxide controls fat deposition in dystrophic skeletal muscle by regulating fibro-adipogenic precursor differentiation.
    Authors: Cordani N, Pisa V, Pozzi L, Sciorati C, Clementi E
    Stem Cells, 2014;32(4):874-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Hypoxia inducible factor-2alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors.
    Authors: Duan L, Takeda K, Fong G
    PLoS ONE, 2014;9(1):e84736.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  30. Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
    Authors: Pinto, Mauricio, Dye, Wendy W, Jacobsen, Britta M, Horwitz, Kathryn
    BMC Cancer, 2014;14(0):735.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Fluorescence
  31. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.
    Authors: Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, Masliah E, Winner B, Wegner M, Winkler J
    Mol Cell Neurosci, 2014;62(0):68-78.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  32. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  33. Abnormal development of NG2+PDGFR-alpha+ neural progenitor cells leads to neonatal hydrocephalus in a ciliopathy mouse model.
    Authors: Carter C, Vogel T, Zhang Q, Seo S, Swiderski R, Moninger T, Cassell M, Thedens D, Keppler-Noreuil K, Nopoulos P, Nishimura D, Searby C, Bugge K, Sheffield V
    Nat Med, 2012;18(12):1797-804.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: IP
  34. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  35. Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke.
    Authors: Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA
    Nat. Med., 2008;14(7):731-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  36. Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis.
    Authors: Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S
    J. Neuroimmunol., 2008;196(1):67-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  37. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006;12(9):2676-88.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  38. Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis.
    Authors: Luyt K, Varadi A, Durant CF, Molnar E
    J. Neurochem., 2006;99(2):641-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Mouse PDGF R alpha Antibody
By Anonymous on 07/27/2018
Application: ICC/IF Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse PDGF R alpha Antibody AF1062
Mouse PDGF R alpha Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse
Western Blot Mouse PDGF R alpha Antibody AF1062
Mouse PDGF R alpha Antibody
By Anonymous on 08/10/2017
Application: IHC Sample Tested: Brain (cerebellum) tissue Species: Mouse