Mouse Plexin C1 Antibody

Catalog # Availability Size / Price Qty
AF5375
AF5375-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Mouse Plexin C1 by Western Blot.
10 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Mouse Plexin C1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Plexin C1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1
Ala35-Thr950
Accession # Q9QZC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Plexin C1 antibody by Western Blot. View Larger

Detection of Mouse Plexin C1 by Western Blot. Western blot shows lysates of mouse thymus tissue and embryonic mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin C1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of Plexin C1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Plexin C1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Immunohistochemistry Plexin C1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Plexin C1 in Mouse Embryo. Plexin C1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurites in the developing midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown Validated Detection of Mouse Plexin C1 by Knockdown Validated View Larger

Detection of Mouse Plexin C1 by Knockdown Validated Sema7A induces the retraction of GnRH neurites through the PlexinC1 transduction pathway and Rap1 inactivation.(a) Representative images of semaphorin-induced neurite retraction in GnV3 neurons transfected with an empty vector (mock), a construct encoding a PlexinC1 short hairpin RNA (GnV3 shRNA PlexinC1) or a Rap1-V12 plasmid encoding a constitutively active form of Rap1. One day following Sema7A treatment (250 ng ml−1), cells were labelled for F-actin (red) to visualize cytoskeletal changes. (b) Quantitative analysis of neurite length in GnV3 cells under different treatment conditions (n=5 independent experiments, n=303 mock-transfected control cells, n=303 mock-transfected cells after Sema7A treatment, unpaired Student’s t-test, ***P<0.0001; n=267 shPlxnC1-treated cells, n=235 shPlxnC1+Sema7A-treated cells, unpaired Student’s t-test, P>0.05; n=131 Rap1-V12-transfected cells, n=115 Rap1-V12-transfected cells+Sema7A, unpaired Student’s t-test, P>0.05). (c) Immunoblotting for markers indicated using transfected GnV3 cells. (d–f) Saline, Sema7A or Sema7A+PlexinC1 was infused (0.2 μg μl−1, 0.5 μl h−1 for 7 days) by stereotaxic implantation of a 28-gauge infusion cannula connected to a subcutaneously implanted mini-osmotic pump in the ME of cycling female rats. Representative oestrous cycle profiles showing the disruption of oestrous cyclicity by the infusion of Sema7A but not of PBS into the ME. Infusion was started on day 9 (downward arrow) and ended 7 days later (upward arrow), when pump contents were exhausted. Die, Diestrus; Es, estrus; Pro, proestrus. (g) Quantitative analysis of alterations in ovarian cyclicity (percentage of time in diestrus) caused by PBS, Sema7A or Sema7A+soluble PlexinC1 infusion into the rat ME (n=6 animals per group, Kruskal–Wallis test, **P<0.005). Scale bar, (a) 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25721933), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin C1

Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively.

Entrez Gene IDs
10154 (Human); 54712 (Mouse); 362873 (Rat)
Alternate Names
CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor

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Citations for Mouse Plexin C1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy
    Authors: N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner
    Cell Reports, 2023-03-21;42(4):112295.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold
    Authors: Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al.
    Neuron
  3. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system
    Authors: N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi
    Nature Communications, 2022-10-13;13(1):6040.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Endothelial semaphorin 7A promotes seawater aspiration-induced acute lung injury through plexin C1 and beta 1 integrin
    Authors: Minlong Zhang, Xue Yan, Wei Liu, Ruilin Sun, Yonghong Xie, Faguang Jin
    Molecular Medicine Reports
  5. Rewiring the taste system
    Authors: H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba
    Nature, 2017-08-09;548(7667):330-333.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2014-11-24;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse Plexin C1 Antibody

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Mouse Plexin C1 Antibody
By Anonymous on 05/12/2017
Application: ELISA Sample Tested: mouse antibody Species: Mouse

Mouse Plexin C1 Antibody
By Anonymous on 08/22/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Dorsal root ganglion Species: Mouse

- 1hr block (room temp.) in antibody diluent (0.2% Triton-X + 5% Donkey Serum + 1% BSA in PBS pH7.4)
- Antibody diluted in above 1:400 and mouse dorsal root ganglia sections incubated overnight at 4oC.
- Sections were washed 3x 5mins then incubated for 2hrs (room temp.) in 1:1000 Donkey anti-Sheep IgG conjugated to Alexa Fluor-488 before washing, and coverslipping.