Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Opossum, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Plexin C1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1
Ala35-Thr950
Accession # Q9QZC2

Specificity

Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Plexin C1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Plexin C1 Antibody

Detection of Mouse Plexin C1 antibody by Western Blot.

Detection of Mouse Plexin C1 by Western Blot.

Western blot shows lysates of mouse thymus tissue and embryonic mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin C1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Plexin C1 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Plexin C1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Plexin C1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Plexin C1 in Mouse Embryo.

Plexin C1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurites in the developing midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Knockdown Validated

Detection of Mouse Plexin C1 by Knockdown Validated

Sema7A induces the retraction of GnRH neurites through the PlexinC1 transduction pathway and Rap1 inactivation.(a) Representative images of semaphorin-induced neurite retraction in GnV3 neurons transfected with an empty vector (mock), a construct encoding a PlexinC1 short hairpin RNA (GnV3 shRNA PlexinC1) or a Rap1-V12 plasmid encoding a constitutively active form of Rap1. One day following Sema7A treatment (250 ng ml−1), cells were labelled for F-actin (red) to visualize cytoskeletal changes. (b) Quantitative analysis of neurite length in GnV3 cells under different treatment conditions (n=5 independent experiments, n=303 mock-transfected control cells, n=303 mock-transfected cells after Sema7A treatment, unpaired Student’s t-test, ***P<0.0001; n=267 shPlxnC1-treated cells, n=235 shPlxnC1+Sema7A-treated cells, unpaired Student’s t-test, P>0.05; n=131 Rap1-V12-transfected cells, n=115 Rap1-V12-transfected cells+Sema7A, unpaired Student’s t-test, P>0.05). (c) Immunoblotting for markers indicated using transfected GnV3 cells. (d–f) Saline, Sema7A or Sema7A+PlexinC1 was infused (0.2 μg μl−1, 0.5 μl h−1 for 7 days) by stereotaxic implantation of a 28-gauge infusion cannula connected to a subcutaneously implanted mini-osmotic pump in the ME of cycling female rats. Representative oestrous cycle profiles showing the disruption of oestrous cyclicity by the infusion of Sema7A but not of PBS into the ME. Infusion was started on day 9 (downward arrow) and ended 7 days later (upward arrow), when pump contents were exhausted. Die, Diestrus; Es, estrus; Pro, proestrus. (g) Quantitative analysis of alterations in ovarian cyclicity (percentage of time in diestrus) caused by PBS, Sema7A or Sema7A+soluble PlexinC1 infusion into the rat ME (n=6 animals per group, Kruskal–Wallis test, **P<0.005). Scale bar, (a) 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25721933), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Plexin C1 by Immunocytochemistry/Immunofluorescence

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Plexin C1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

1 µg/mL
Sample: Mouse thymus tissue and embryonic mouse heart tissue

Reviewed Applications

Read 2 reviews rated 4.5 using AF5375 in the following applications:

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin C1

Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively.

Alternate Names

CD232, PLXNC1, VESPR

Entrez Gene IDs

10154 (Human); 54712 (Mouse); 362873 (Rat)

Gene Symbol

PLXNC1

UniProt

Q9QZC2

Additional Plexin C1 Products

Product Documents for Mouse Plexin C1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Plexin C1 Antibody

For research use only

Citations for Mouse Plexin C1 Antibody

Customer Reviews for Mouse Plexin C1 Antibody (2)

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  • Mouse Plexin C1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: mouse antibody
    Species: Mouse
    Verified Customer | Posted 05/12/2017
  • Mouse Plexin C1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Dorsal root ganglion
    Species: Mouse
    Verified Customer | Posted 08/22/2016
    - 1hr block (room temp.) in antibody diluent (0.2% Triton-X + 5% Donkey Serum + 1% BSA in PBS pH7.4) - Antibody diluted in above 1:400 and mouse dorsal root ganglia sections incubated overnight at 4oC. - Sections were washed 3x 5mins then incubated for 2hrs (room temp.) in 1:1000 Donkey anti-Sheep IgG conjugated to Alexa Fluor-488 before washing, and coverslipping.
    Mouse Plexin C1 Antibody AF5375

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