PLTP in Mouse Brain.
PLTP was detected in perfusion fixed frozen sections of mouse brain (medulla) using Sheep Anti-Mouse PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PLTP (phospholipid transfer protein) is a 65-80 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature mouse PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain (aa 21-239), a C-terminal HDL-binding domain (aa 263-463), and one essential intrachain disulfide bond (Cys146‑Cys185). There are three potential PLTP splice variants. One shows a deletion of aa 148-493, a second shows a deletion of aa 111-162, and a third shows a deletion of aa 14-33 plus a two aa substitution for aa 184-493. Over aa 18-493, mouse PLTP shares 92% and 83% aa identity with rat and human PLTP, respectively.
Phospholipid Transfer Protein
Entrez Gene IDs:
5360 (Human); 18830 (Mouse); 296371 (Rat)
HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP
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