Mouse PLTP Antibody

Catalog # Availability Size / Price Qty
AF4918
AF4918-SP
PLTP in Mouse Brain.
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Mouse PLTP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PLTP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human PLTP is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PLTP
Glu18-Ala493 (Asp32Glu)
Accession # P55065
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PLTP (Catalog # 4918-PL)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry PLTP in Mouse Brain. View Larger

PLTP in Mouse Brain. PLTP was detected in perfusion fixed frozen sections of mouse brain (medulla) using Sheep Anti-Mouse PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLTP

PLTP (phospholipid transfer protein) is a 65-80 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature mouse PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain (aa 21-239), a C-terminal HDL-binding domain (aa 263-463), and one essential intrachain disulfide bond (Cys146‑Cys185). There are three potential PLTP splice variants. One shows a deletion of aa 148-493, a second shows a deletion of aa 111-162, and a third shows a deletion of aa 14-33 plus a two aa substitution for aa 184-493. Over aa 18-493, mouse PLTP shares 92% and 83% aa identity with rat and human PLTP, respectively.

Long Name
Phospholipid Transfer Protein
Entrez Gene IDs
5360 (Human); 18830 (Mouse); 296371 (Rat)
Alternate Names
HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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