Mouse PLUNC Antibody

Catalog # Availability Size / Price Qty
AF4274
AF4274-SP
Detection of Mouse PLUNC by Western Blot.
4 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse PLUNC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PLUNC in direct ELISAs and Western blots. In these formats, less than 10% cross-reactivity with recombinant human PLUNC is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse PLUNC
Ala59-Val278
Accession # NP_035256
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PLUNC antibody by Western Blot. View Larger

Detection of Mouse PLUNC by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLUNC at approximately 30kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry PLUNC antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PLUNC in Mouse Embryo. PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse PLUNC by Western Blot View Larger

Detection of Mouse PLUNC by Western Blot A1AT treatment enhances SPLUNC1 and reduces NE activity in bronchoalveolar lavage (BAL) fluid of PA-infected WT mice. PA-infected mice were treated with A1AT for 22 hrs and sacrificed after 24 hrs of infection as described in Materials and Methods. Quantitative analysis (A) and representative image (B) of BAL fluid SPLUNC1 Western blot were shown to demonstrate the therapeutic effect of A1AT treatment. The two lanes of Western blot image under saline or PA treatment represent SPLUNC1 data from two different mice. NE activity (C) was examined by an NE activity assay. N = 4 – 7 mice per group. The vertical dotted red line in Figure 2B separates the saline group from the PA infection group. NS indicates no significant differences. Data are expressed as means ± SEM. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-122), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse PLUNC by Western Blot View Larger

Detection of Mouse PLUNC by Western Blot Pseudomonas aeruginosa (PA) infection reduces SPLUNC1 and increases neutrophil elastase (NE) activity in bronchoalveolar lavage (BAL) fluid of wild-type (WT) mice. BAL fluid from WT mice was processed for SPLUNC1 protein Western blot. (A) – Quantitative analysis of BAL fluid SPLUNC1 protein expression normalized by albumin. (B) – Representative Western blot image of SPLUNC1 and albumin. The vertical dotted red line separates the saline group from the PA infection group. The two lanes under saline or PA treatment represent SPLUNC1 data from two different mice. (C) – NE activity was examined by an NE activity assay as described in Materials and methods. N = 4 – 5 mice per group. Data are expressed as means ± SEM. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-122), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLUNC

PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.

Long Name
Palate, Lung, and Nasal Epithelium Associated
Entrez Gene IDs
51297 (Human)
Alternate Names
bA49G10.5; BPIFA1; ligand-binding protein RYA3; LPLUNC3; Lung-specific protein X; LUNX; LUNXNASG; Nasopharyngeal carcinoma-related protein; Palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; palate, lung and nasal epithelium carcinoma associated; PLUNC; protein Plunc; Secretory protein in upper respiratory tracts; SPLUNC1; SPLUNC1SPURT; tracheal epithelium enriched protein; Tracheal epithelium-enriched protein; Von Ebner protein Hl

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Citations for Mouse PLUNC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. alpha1-Antitrypsin promotes SPLUNC1-mediated lung defense against Pseudomonas aeruginosa infection in mice.
    Authors: Jiang D, Persinger R, Wu Q, Gross A, Chu H
    Respir Res, 2013-11-09;14(0):122.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  2. SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection.
    Authors: Liu Y, Bartlett J, Di M, Bomberger J, Chan Y, Gakhar L, Mallampalli R, McCray P, Di Y
    Am J Pathol, 2013-03-15;182(5):1519-31.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  3. Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
    Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
    Mucosal Immunol, 2012-05-02;5(5):524-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse PLUNC Antibody
By Anonymous on 02/19/2021
Application: WB Sample Tested: Cell Lysates Species: Mouse