Detection of Mouse PLUNC by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLUNC at approximately 30kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PLUNC in Mouse Embryo.
PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.
Palate, Lung, and Nasal Epithelium Associated
Entrez Gene IDs:
bA49G10.5; BPIFA1; ligand-binding protein RYA3; LPLUNC3; Lung-specific protein X; LUNX; LUNXNASG; Nasopharyngeal carcinoma-related protein; Palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; palate, lung and nasal epithelium carcinoma associated; PLUNC; protein Plunc; Secretory protein in upper respiratory tracts; SPLUNC1; SPLUNC1SPURT; tracheal epithelium enriched protein; Tracheal epithelium-enriched protein; Von Ebner protein Hl
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