Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PLUNC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PLUNC
Ala59-Val278
Accession # NP_035256

Specificity

Detects mouse PLUNC in direct ELISAs and Western blots. In these formats, less than 10% cross-reactivity with recombinant human PLUNC is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse PLUNC Antibody

Detection of Mouse PLUNC antibody by Western Blot.

Detection of Mouse PLUNC by Western Blot.

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLUNC at approximately 30kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PLUNC antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

PLUNC in Mouse Embryo.

PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse PLUNC by Western Blot

Detection of Mouse PLUNC by Western Blot

A1AT treatment enhances SPLUNC1 and reduces NE activity in bronchoalveolar lavage (BAL) fluid of PA-infected WT mice. PA-infected mice were treated with A1AT for 22 hrs and sacrificed after 24 hrs of infection as described in Materials and Methods. Quantitative analysis (A) and representative image (B) of BAL fluid SPLUNC1 Western blot were shown to demonstrate the therapeutic effect of A1AT treatment. The two lanes of Western blot image under saline or PA treatment represent SPLUNC1 data from two different mice. NE activity (C) was examined by an NE activity assay. N = 4 – 7 mice per group. The vertical dotted red line in Figure 2B separates the saline group from the PA infection group. NS indicates no significant differences. Data are expressed as means ± SEM. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-99…), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse PLUNC by Western Blot

Detection of Mouse PLUNC by Western Blot

Pseudomonas aeruginosa (PA) infection reduces SPLUNC1 and increases neutrophil elastase (NE) activity in bronchoalveolar lavage (BAL) fluid of wild-type (WT) mice. BAL fluid from WT mice was processed for SPLUNC1 protein Western blot. (A) – Quantitative analysis of BAL fluid SPLUNC1 protein expression normalized by albumin. (B) – Representative Western blot image of SPLUNC1 and albumin. The vertical dotted red line separates the saline group from the PA infection group. The two lanes under saline or PA treatment represent SPLUNC1 data from two different mice. (C) – NE activity was examined by an NE activity assay as described in Materials and methods. N = 4 – 5 mice per group. Data are expressed as means ± SEM. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-99…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse PLUNC Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen cross-sections of mouse embryo nose (13 d.p.c.)

Western Blot

1 µg/mL
Sample: Mouse lung tissue

Reviewed Applications

Read 1 review rated 5 using AF4274 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLUNC

PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.

Long Name

Palate, Lung, and Nasal Epithelium Associated

Alternate Names

BPIFA1, LUNX, SPLUNC1

Entrez Gene IDs

51297 (Human)

Gene Symbol

BPIFA1

UniProt

NP_035256

Additional PLUNC Products

Product Documents for Mouse PLUNC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PLUNC Antibody

For research use only

Citations for Mouse PLUNC Antibody

Customer Reviews for Mouse PLUNC Antibody (1)

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  • Mouse PLUNC Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 02/19/2021
    Mouse PLUNC Antibody AF4274

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