Mouse PLUNC Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PLUNC in direct ELISAs and Western blots. In these formats, less than 10% cross-reactivity with recombinant human PLUNC is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse PLUNC
    Ala59-Val278
    Accession # NP_035256
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse PLUNC by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLUNC at approximately 30kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
PLUNC in Mouse Embryo. PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLUNC

PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.

  • Long Name:
    Palate, Lung, and Nasal Epithelium Associated
  • Entrez Gene IDs:
    51297 (Human)
  • Alternate Names:
    bA49G10.5; BPIFA1; ligand-binding protein RYA3; LPLUNC3; Lung-specific protein X; LUNX; LUNXNASG; Nasopharyngeal carcinoma-related protein; Palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; palate, lung and nasal epithelium carcinoma associated; PLUNC; protein Plunc; Secretory protein in upper respiratory tracts; SPLUNC1; SPLUNC1SPURT; tracheal epithelium enriched protein; Tracheal epithelium-enriched protein; Von Ebner protein Hl
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. alpha1-Antitrypsin promotes SPLUNC1-mediated lung defense against Pseudomonas aeruginosa infection in mice.
    Authors: Jiang D, Persinger R, Wu Q, Gross A, Chu H
    Respir Res, 2013;14(0):122.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  2. SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection.
    Authors: Liu Y, Bartlett J, Di M, Bomberger J, Chan Y, Gakhar L, Mallampalli R, McCray P, Di Y
    Am J Pathol, 2013;182(5):1519-31.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  3. Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
    Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
    Mucosal Immunol, 2012;5(5):524-34.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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