|Detection of Mouse PU.1/Spi‑1 by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PU.1/Spi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7124) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PU.1/Spi‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Spi1 (SFFV proviral integration 1 protein; also PU.1 and Sfpi1) is a 37-41 kDa member of the ets family of transcription factors. It is both an RNA and DNA binding protein that is found in hematopoietic cells such as B cells, neutrophils, macrophages and dendritic cells (DC). Spi1 can act as both a transcriptional activator and repressor. In DC, Spi1 promotes the expression of CD80, CD86 and CD11b, while in proerythroblasts, it blocks GATA-1 induced transcriptional activation. Spi1 binds to DNA as a monomer, and interacts with multiple factors such as Runx-1, IRF8, GATA-1 and c-Jun. Mouse Spi1 is 272 amino acids (aa) in length. It contains a transactivation region (aa 7-117), a PEST domain (aa 118-164), and a DNA-binding domain (aa 165-266). Phosphorylation on Ser41, 142 and 148 increases activity. There is one alternative start site at Met7. Over aa 1-169, mouse Spi1 shares 88% and 81% aa identity with rat and human Spi1, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided