Mouse PU.1/Spi‑1 Antibody

Name: Mouse PU.1/Spi‑1 Antibody
Brand: R&D Systems
SKU: AF7124
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF7124

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all PU.1/Spi-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PU.1/Spi-1
Met1-Lys169
Accession # P17433

Specificity

Detects mouse PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Spi-1 is observed, and less than 1% cross-reactivity with recombinant mouse Spi-B is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse PU.1/Spi‑1 Antibody

Detection of Mouse PU.1/Spi‑1 by Western Blot.

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PU.1/Spi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7124) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PU.1/Spi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse PU.1/Spi‑1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PU.1/Spi-1

Spi1 (SFFV proviral integration 1 protein; also PU.1 and Sfpi1) is a 37-41 kDa member of the ets family of transcription factors. It is both an RNA and DNA binding protein that is found in hematopoietic cells such as B cells, neutrophils, macrophages and dendritic cells (DC). Spi1 can act as both a transcriptional activator and repressor. In DC, Spi1 promotes the expression of CD80, CD86 and CD11b, while in proerythroblasts, it blocks GATA-1 induced transcriptional activation. Spi1 binds to DNA as a monomer, and interacts with multiple factors such as Runx-1, IRF8, GATA-1 and c-Jun. Mouse Spi1 is 272 amino acids (aa) in length. It contains a transactivation region (aa 7-117), a PEST domain (aa 118-164), and a DNA-binding domain (aa 165-266). Phosphorylation on Ser41, 142 and 148 increases activity. There is one alternative start site at Met7. Over aa 1-169, mouse Spi1 shares 88% and 81% aa identity with rat and human Spi1, respectively.