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Mouse RAGE Alexa Fluor® 488-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of RAGE in Mouse Splenocytes by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse RAGE in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant canine RAGE and no cross-reactivity with recombinant human RAGE or recombinant rat RAGE is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 697023
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse RAGE
    Gly23-Leu342
    Accession # NP_031451
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets
Product Datasheet

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry RAGE Antibody (697023) [Alexa Fluor® 488]
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Detection of RAGE in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th1 cells were stained with Rat Anti-Mouse RAGE Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB11795G) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: RAGE

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

  • References:
    1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
    2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
    3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
    4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
    5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
    6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
    7. Valencia, J.V. et al. (2004) Diabetes 53:743.
  • Long Name:
    Receptor for Advanced Glycation End Products
  • Entrez Gene IDs:
    177 (Human); 11596 (Mouse); 81722 (Rat)
  • Alternate Names:
    AGER; EC 2.7.11.22; MOK Protein Kinase; MOK; RAGE; RAGE-1; Renal tumor antigen 1; renal tumor antigen
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

 Ctrl IC006G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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