Mouse/Rat ADNP Antibody

R&D Systems | Catalog # AF5919

R&D Systems
Discontinued Product
AF5919 has been discontinued. View all ADNP products.

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Co-Immunoprecipitation, Westen Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ADNP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ADNP
Leu941-Ala1102
Accession # Q9H2P0

Specificity

Detects mouse and rat ADNP in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human ADNP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat ADNP Antibody

Detection of Mouse/Rat ADNP antibody by Western Blot.

Detection of Mouse/Rat ADNP by Western Blot.

Western blot shows lysates of embryonic rat cortical neuron/ glial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ADNP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ADNP antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

ADNP in RAW 264.7 Mouse Cell Line.

ADNP was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ADNP antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

ADNP in Mouse Brain.

ADNP was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Specific staining was localized to Purkinje cells and neurons in molecular cell layer. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of ADNP in Mouse Brain.

ADNP was detected in immersion fixed paraffin-embedded sections of mouse brain using Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse/Rat ADNP Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse brain

Western Blot

1 µg/mL
Sample: Embryonic rat cortical neuron/ glial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADNP

ADNP (Activity-dependent neuroprotective protein) is an ADNF-related factor found in astrocytes and microvascular endothelial cells. Although its predicted MW is 124 kDa, it runs anomalously at 148‑150 kDa in SDS-PAGE. ADNP is inducible by VIP in astrocytes, and serves as a neuroprotector, both outside and inside the cell. Extracellularly, ADNP may protect synapse activity; intracellularly, ADNP interacts with tubulin, promoting microtubule assembly, and within the nucleus, it contributes to the SWI/SNF chromatin remodeling complex. Mouse ADNP is 1108 amino acids (aa) in length. It contains a neuroprotective peptide/NAP (aa 354‑361) embedded between nine C2H2-type Zn finger domains (aa 76‑685), a DNA-binding homeobox region (aa 752‑813) and a Leu-rich NLS that overlaps a protein internalization sequence (aa 787‑812). There are alternative start sites at Met229 and Met281. Over aa 947‑1108, mouse ADNP shares 98% and 93% aa identity with rat and human ADNP, respectively.

Long Name

Activity-dependent Neuroprotective Protein

Alternate Names

ADNP1

Entrez Gene IDs

23394 (Human); 11538 (Mouse)

Gene Symbol

ADNP

UniProt

Q9H2P0 (Human)

Additional ADNP Products

Product Documents for Mouse/Rat ADNP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat ADNP Antibody

For research use only

Citations for Mouse/Rat ADNP Antibody

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Protocols

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