Mouse/Rat TLR7 Antibody

(1 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse TLR7 in direct ELISAs and mouse and rat TLR7 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human TLR7 or recombinant mouse TLR8 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 726606
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TLR7
    Asn275-Phe444
    Accession # P58681
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat TLR7 by Western Blot. Western blot shows lysates of mouse thymus tissue and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat TLR7 Monoclonal Antibody (Catalog # MAB7156) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for TLR7 at approximately 130 to 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR7
TLR7 (Toll-like receptor 7) is an approximately 130 kDa type I transmembrane glycoprotein and member of the Toll-like receptor family. Mouse TLR7 cDNA encodes 1050 amino acids (aa) including a 26 aa signal sequence and a 811 aa extracellular domain (ECD) with 28 leucine-rich repeats (LRR). Within the region used as an immunogen (LRR 10-16), mouse TLR7 shares 73% and 88% aa identity with human and rat TLR7, respectively. TLR7 mRNA is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid dendritic cells, B cells and eosinophils. TLR7 participates in the innate immune response to microbial agents by recognizing viral and nonviral single stranded RNA.
  • Long Name:
    Toll-like Receptor 7
  • Entrez Gene IDs:
    51284 (Human); 170743 (Mouse); 317468 (Rat)
  • Alternate Names:
    TLR7; toll-like receptor 7
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015;45(6):1842-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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