Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 726606
View all TLR7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TLR7
Asn275-Phe444
Accession # P58681

Specificity

Detects mouse TLR7 in direct ELISAs and mouse and rat TLR7 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human TLR7 or recombinant mouse TLR8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse/Rat TLR7 Antibody

Detection of Mouse and Rat TLR7 antibody by Western Blot.

Detection of Mouse and Rat TLR7 by Western Blot.

Western blot shows lysates of mouse thymus tissue and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat TLR7 Monoclonal Antibody (Catalog # MAB7156) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for TLR7 at approximately 130 to 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse/Rat TLR7 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse thymus tissue and NR8383 rat alveolar macrophage cell line

Reviewed Applications

Read 1 review rated 5 using MAB7156 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR7

TLR7 (Toll-like receptor 7) is an approximately 130 kDa type I transmembrane glycoprotein and member of the Toll-like receptor family. Mouse TLR7 cDNA encodes 1050 amino acids (aa) including a 26 aa signal sequence and a 811 aa extracellular domain (ECD) with 28 leucine-rich repeats (LRR). Within the region used as an immunogen (LRR 10-16), mouse TLR7 shares 73% and 88% aa identity with human and rat TLR7, respectively. TLR7 mRNA is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid dendritic cells, B cells and eosinophils. TLR7 participates in the innate immune response to microbial agents by recognizing viral and nonviral single stranded RNA.

Long Name

Toll-like Receptor 7

Alternate Names

toll-like receptor 7

Entrez Gene IDs

51284 (Human); 170743 (Mouse); 317468 (Rat)

Gene Symbol

TLR7

UniProt

P58681

Additional TLR7 Products

Product Documents for Mouse/Rat TLR7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat TLR7 Antibody

For research use only

Citations for Mouse/Rat TLR7 Antibody

Customer Reviews for Mouse/Rat TLR7 Antibody (1)

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  • Mouse/Rat TLR7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Thymus tissue
    Species: Mouse
    Verified Customer | Posted 01/06/2022
    Mouse/Rat TLR7 Antibody MAB7156

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