Mouse Relaxin-1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Relaxin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Relaxin-1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Relaxin-1
    Arg23-Arg54 with an N-terminal Met (B chain) & Glu161-Cys185 (A chain)
    Accession # NP_035402
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Relaxin‑1 by Western Blot. Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prorelaxin‑1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Relaxin‑1 in Mouse Testis. Relaxin‑1 was detected in immersion fixed frozen sections of adult mouse testis using Sheep Anti-Mouse Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6637) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Relaxin-1

Mouse Relaxin-1 (RLN1 or M1 relaxin) is the counterpart of human relaxin-2 (H2 relaxin) within the structurally related insulin/relaxin superfamily, while orthologs of the human relaxin-1 are found only in higher primates (1, 2). As with other insulin/relaxin superfamily members, RLN1 is synthesized as a preprohormone (1‑3, 5). Processing of the 21 kDa preprorelaxin-1 and 16-17 kDa prorelaxin-1 includes removal of the signal sequence, formation of two disulfide bonds between A and B chains and removal of the intervening C‑chain by a prohormone convertase. The resulting mature protein is an unglycosylated 6 kDa dimer of disulfide-linked A and B chains that binds the leucine-rich G-protein coupled receptor RXFP1, previously called LGR7 (1‑4). Mouse RLN1 shares only 67%, 39%, 36% and 42% amino acid (aa) identity with rat, equine and feline RLN1 and human Relaxin-2, respectively, and its activity shows partial species specificity. For example, a unique amino acid near the end of the A chain in mice, Tyr184, lowers its affinity for RXFP1 compared to other species (5). Mouse RLN1 is prominently expressed in the prostate and ovary, with lower levels in the brain, heart and other organs (1‑3). In the prostate, RLN1 is anti-apoptotic and contributes to development and maintenance of male fertility (6). In the female mouse, circulating RLN1 produced by the corpus luteum during pregnancy is essential for growth and softening of the cervix and vagina in preparation for delivery. It also promotes development of the mouse mammary apparatus, regulates plasma osmolality, and increases cardiac output and glomerular filtration rate in pregnancy (1, 2). Many RLN1 effects on reproductive tissues are augmented by estrogen (1‑3, 7). In non‑reproductive tissues, RLN1 mediates collagen turnover (7). RLN1-deficient mice develop age‑related fibrosis and smooth muscle hypertrophy in organs such as lung, heart, kidney and liver (7‑10).

  • References:
    1. Kong, R.C.K. et al. (2010) Mol. Cell. Endocrinol. 320:1.
    2. Sherwood, O.D. (2004) Endocr. Rev. 25:205.
    3. Callander, G.E. and R.A.D. Bathgate (2010) Cell. Mol. Life Sci. 67:2327.
    4. Novak, J. et al. (2006) FASEB J. 20:2352.
    5. Schwabe, C. and E. E. Bullesbach (1994) FASEB J. 8:1152.
    6. Samuel, C.S. et al. (2003) Lab. Invest. 83:1055.
    7. Lekgabe, E.D. et al. (2006) Endocrinology 147:5575.
    8. Samuel, C.S. et al. (2003) FASEB J. 17:121.
    9. Bennett, R.G. (2009) Transl. Res. 154:1.
    10. Hewitson, T.D. et al. (2007) Endocrinology 148:660.
  • Entrez Gene IDs:
    6013 (Human); 19773 (Mouse); 25616 (Rat)
  • Alternate Names:
    bA12D24.3.1; bA12D24.3.2; H1; preprorelaxin H1; prorelaxin H1; relaxin 1 (H1); relaxin 1; relaxin H1; Relaxin1; Relaxin-1; RLN1; RLXH1
Related Research Areas
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse Relaxin-1 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information