Detects mouse Semaphorin 4G in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Semaphorin 4G is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4C, and rmSemaphorin 4F is observed.
Detection of Mouse Semaphorin 4G by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6504) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4G at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1
Semaphorin 4G in Mouse Embryo. Semaphorin 4G was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Semaphorin 4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6504) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (cortex) and connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4G
Semaphorin 4G (Sema4G) is a 92 kDa (predicted), class IV member of the semaphorin family of proteins. It is expressed by select cell types, including oligodendroglia and Purkinje neurons, and likely acts as a chemorepellent by binding to B-type plexins. Mature mouse Sema4G is a type I transmembrane protein that is 820 amino acids (aa) in length. It contains a 656 aa extracellular region (aa 18-673) that is characterized by one Sema domain (aa 35-503), a PSI region (aa 505‑556), and an Ig-like C2-type domain (aa 565-648). The 143 aa cytoplasmic region contains a poly-proline segment and a Ser phosphorylation site. Over aa 18‑673, mouse Sema4G shares 91% and 97% aa identity with human and rat Sema4G, respectively.
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