Mouse Semaphorin 6A Antibody

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Product Details
Citations (2)
Supplemental Products
Reviews (1)

Mouse Semaphorin 6A Antibody Summary

Species Reactivity
Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) Semaphorin 6A is observed and no cross-reactivity with recombinant mouse Semaphorins 3C, 3E, 3F or 7A, or rhSemaphorins 3A, or 3B is observed.
Monoclonal Rat IgG2A Clone # 201932
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Accession # O35464
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Semaphorin 6A
8-25 µg/mL
Perfusion fixed frozen sections of mouse brain (medulla) and mouse embryo

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is a member of the class 6 semaphorin family of type I transmembrane proteins important in axon guidance during neuronal development. Class 6 semaphorins mediate bidirectional signaling, in part through interaction with plexin A1. In addition to neuronal expression, Semaphorin 6A is induced by IFN-gamma in Langerhans cells. Mature mouse and human Semaphorin 6A extracellular domains share 95% amino acid sequence identity.

Entrez Gene IDs
57556 (Human); 20358 (Mouse)
Alternate Names
KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

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Citations for Mouse Semaphorin 6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells.
    Authors: Renaud J, Kerjan G, Sumita I, Zagar Y, Georget V, Kim D, Fouquet C, Suda K, Sanbo M, Suto F, Ackerman SL, Mitchell KJ, Fujisawa H, Chedotal A
    Nat. Neurosci., 2008-03-09;11(4):440-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Plexin-B2 controls the development of cerebellar granule cells.
    Authors: Friedel RH, Kerjan G, Rayburn H, Schuller U, Sotelo C, Tessier-Lavigne M, Chedotal A
    J. Neurosci., 2007-04-04;27(14):3921-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Mouse Semaphorin 6A Antibody
By Anonymous on 06/17/2022
Application: WB Sample Tested: Cortical neurons Species: Mouse