Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 201932
View all Semaphorin 6A Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464

Specificity

Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) Semaphorin 6A is observed and no cross-reactivity with recombinant mouse Semaphorins 3C, 3E, 3F or 7A, or rhSemaphorins 3A, or 3B is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Semaphorin 6A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (medulla) and mouse embryo

Western Blot

1 µg/mL
Sample: Recombinant Mouse Semaphorin 6A

Reviewed Applications

Read 1 review rated 5 using MAB1615 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is a member of the class 6 semaphorin family of type I transmembrane proteins important in axon guidance during neuronal development. Class 6 semaphorins mediate bidirectional signaling, in part through interaction with plexin A1. In addition to neuronal expression, Semaphorin 6A is induced by IFN-gamma in Langerhans cells. Mature mouse and human Semaphorin 6A extracellular domains share 95% amino acid sequence identity.

Alternate Names

Sema6A

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Gene Symbol

SEMA6A

UniProt

O35464

Additional Semaphorin 6A Products

Product Documents for Mouse Semaphorin 6A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Semaphorin 6A Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Semaphorin 6A Antibody

Customer Reviews for Mouse Semaphorin 6A Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
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1 Stars
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  • Mouse Semaphorin 6A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 06/17/2022
    Mouse Semaphorin 6A Antibody MAB1615

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Protocols

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FAQs

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