Mouse SEZ6L Antibody

Catalog # Availability Size / Price Qty
AF4804
AF4804-SP
SEZ6L in Mouse Brain.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse SEZ6L Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SEZ6L in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L
Glu32-Ser889
Accession # AAH65117
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry SEZ6L antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

SEZ6L in Mouse Brain. SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L

SEZ6L (Seizure 6-Like protein; also BSRP-B/Brain-specific receptor-like protein B) is a 200-210 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, particularly cerebellar interneurons plus Purkinje and granule cells. SEZ6L likely regulates Ca++ availability and thus impacts PKC alpha activation and mGluR1 signaling. Mature mouse SEZ6L is a 932 amino acid (aa) type I transmembrane protein. It contains an 866 aa extracellular domain (ECD) (aa 32-897) and a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There is one 190-200 kDa splice variant termed BSRP-B that contains a Phe substitution for aa 807-872 of the fifth SCR domain. Over aa 32-889, mouse SEZ6L shares 80% and 97% aa identity with human and rat SEZ6L, respectively.

Long Name
Seizure-related 6 Homolog-like
Entrez Gene IDs
23544 (Human); 56747 (Mouse); 304554 (Rat)
Alternate Names
Acig1; AIG1; Aig1L; BSRP-B; seizure related 6 homolog (mouse)-like; seizure related gene 6-like; SEZ6L

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse SEZ6L Antibody

There are currently no reviews for this product. Be the first to review Mouse SEZ6L Antibody and earn rewards!

Have you used Mouse SEZ6L Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review