SEZ6L2/BSRP-A in Mouse Brain. SEZ6L2/BSRP-A was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using 25 µg/mL Rat Anti-Mouse SEZ6L2/BSRP-A Monoclonal Antibody (Catalog # MAB4916) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse SEZ6L2/BSRP-A by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SEZ6L2/BSRP-A Monoclonal Antibody (Catalog # MAB4916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SEZ6L2/BSRP-A at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BSRP-A (Brain-specific receptor-like protein A; also known as SEZ6L2) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, and likely regulates PKC alpha activation. Mature mouse BSRP-A is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for BSRP-A. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse BSRP-A shares 96% and 99% aa sequence identity with human and rat BSRP-A, respectively.
Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A
Entrez Gene IDs:
26470 (Human); 233878 (Mouse); 308988 (Rat)
BSRPA; BSRP-A; PSK-1; seizure 6-like protein 2; seizure related 6 homolog (mouse)-like 2; SEZ6L2; UNQ1903/PRO4349
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