Mouse SorCS2 Antibody

Catalog # Availability Size / Price Qty
AF4237
AF4237-SP
SorCS2 in Mouse Brain.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse SorCS2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SorCS2
Ala118-Gly1078
Accession # Q9EPR5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SorCS2 (Catalog # 4237-SR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry SorCS2 in Mouse Brain. View Larger

SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry SorCS2 in Mouse Brain. View Larger

SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS2

Mouse SorCS2 is a 110‑130 kDa member of the Vps10p (vacuolar protein sorting 10 protein)-D family of sorting receptors. It is synthesized as a 1159 amino acid (aa) preproprecursor that undergoes proteolytic processing to generate a 1042 aa mature type I transmembrane receptor. The extracellular domain shows an imperfect LRR and a Vps10p domain. SorCS2 is plasma membrane expressed and may undergo proteolytic cleavage. Over aa 118-1078, mouse SorCS2 is 88% and 98% aa identical to human and rat SorCs2, respectively.

Long Name
Sortilin-related VPS10 Domain Containing Receptor 2
Entrez Gene IDs
57537 (Human); 81840 (Mouse)
Alternate Names
KIAA1329VPS10 domain receptor protein; SorCS2; sortilin-related VPS10 domain containing receptor 2; VPS10 domain-containing receptor SorCS2

Product Datasheets

Citations for Mouse SorCS2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017;13(6):e1006470.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  2. SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease
    Authors: Q Ma, J Yang, TA Milner, JG Vonsattel, ME Palko, L Tessarollo, BL Hempstead
    JCI Insight, 2017;2(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  3. Disruption of SorCS2 reveals differences in the regulation of stereociliary bundle formation between hair cell types in the inner ear
    Authors: A Forge, RR Taylor, SJ Dawson, M Lovett, DJ Jagger
    PLoS Genet, 2017;13(3):e1006692.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Fresh

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Secondary Antibodies

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