Mouse SorCS2 is a 110‑130 kDa member of the Vps10p (vacuolar protein sorting 10 protein)-D family of sorting receptors. It is synthesized as a 1159 amino acid (aa) preproprecursor that undergoes proteolytic processing to generate a 1042 aa mature type I transmembrane receptor. The extracellular domain shows an imperfect LRR and a Vps10p domain. SorCS2 is plasma membrane expressed and may undergo proteolytic cleavage. Over aa 118-1078, mouse SorCS2 is 88% and 98% aa identical to human and rat SorCs2, respectively.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SorCS2
Ala118-Gly1078
Accession # Q9EPR5
Ala118-Gly1078
Accession # Q9EPR5
Specificity
Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse SorCS2 Antibody
SorCS2 in Mouse Brain.
SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
SorCS2 in Mouse Brain.
SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Applications for Mouse SorCS2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cortex)
Sample: Perfusion fixed frozen sections of mouse brain (cortex)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse SorCS2 (Catalog # 4237-SR)
Sample: Recombinant Mouse SorCS2 (Catalog # 4237-SR)
Reviewed Applications
Read 5 reviews rated 4.4 using AF4237 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SorCS2
Long Name
Sortilin-related VPS10 Domain Containing Receptor 2
Alternate Names
KIAA1329VPS10 domain receptor protein, sortilin-related VPS10 domain containing receptor 2, VPS10 domain-containing receptor SorCS2
Gene Symbol
SORCS2
UniProt
Additional SorCS2 Products
Product Documents for Mouse SorCS2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse SorCS2 Antibody
For research use only
Citations for Mouse SorCS2 Antibody
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Customer Reviews for Mouse SorCS2 Antibody (5)
4.4 out of 5
5 Customer Ratings
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Application: Western BlotSample Tested: Hippocampal lysates from p20 miceSpecies: MouseVerified Customer | Posted 12/17/2014
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Application: Western BlotSample Tested: Primary rat Schwann cellsSpecies: RatVerified Customer | Posted 12/17/2014
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Application: ImmunoprecipitationSample Tested: IP of endogenous SorCS2 in mouse tissues as well as SorCS2 knockout mouseSpecies: MouseVerified Customer | Posted 12/17/2014
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Application: ImmunocytochemistrySample Tested: Mouse TissuesSpecies: MouseVerified Customer | Posted 12/16/2014
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Application: Western BlotSample Tested: mouse tissue homogenatesSpecies: OtherVerified Customer | Posted 12/16/2014
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars