Mouse SorCS2 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SorCS2
    Ala118-Gly1078
    Accession # Q9EPR5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse SorCS2 (Catalog # 4237-SR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SorCS2

Mouse SorCS2 is a 110‑130 kDa member of the Vps10p (vacuolar protein sorting 10 protein)-D family of sorting receptors. It is synthesized as a 1159 amino acid (aa) preproprecursor that undergoes proteolytic processing to generate a 1042 aa mature type I transmembrane receptor. The extracellular domain shows an imperfect LRR and a Vps10p domain. SorCS2 is plasma membrane expressed and may undergo proteolytic cleavage. Over aa 118-1078, mouse SorCS2 is 88% and 98% aa identical to human and rat SorCs2, respectively.

  • Long Name:
    Sortilin-related VPS10 Domain Containing Receptor 2
  • Entrez Gene IDs:
    57537 (Human); 81840 (Mouse)
  • Alternate Names:
    KIAA1329VPS10 domain receptor protein; SorCS2; sortilin-related VPS10 domain containing receptor 2; VPS10 domain-containing receptor SorCS2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Disruption of SorCS2 reveals differences in the regulation of stereociliary bundle formation between hair cell types in the inner ear
    Authors: A Forge, RR Taylor, SJ Dawson, M Lovett, DJ Jagger
    PLoS Genet, 2017;13(3):e1006692.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  2. SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease
    Authors: Q Ma, J Yang, TA Milner, JG Vonsattel, ME Palko, L Tessarollo, BL Hempstead
    JCI Insight, 2017;2(9):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017;13(6):e1006470.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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