Mouse Spi-B Antibody

Detection of Mouse Spi-B by Immunocytochemistry/Immunofluorescence

RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Spi-B by Immunocytochemistry/Immunofluorescence

RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.