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Mouse TCF-3/E2A Antibody

Catalog #: MAB7650
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7650
MAB7650-SP

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Detection of Mouse TCF‑3/E2A by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TCF-3/E2A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TCF‑3/E2A in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TCF-3 is observed.
Source
Monoclonal Rat IgG2A Clone # 826927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TCF‑3/E2A
Asn33-Arg159
Accession # P15806
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TCF-3/E2A antibody by Western Blot. View Larger

Detection of Mouse TCF‑3/E2A by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TCF-3/E2A Monoclonal Antibody (Catalog # MAB7650) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TCF-3/E2A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TCF-3/E2A antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

TCF‑3/E2A in Mouse Embryo. TCF-3/E2A was detected in immersion fixed frozen sections of E9.5 mouse embryo using Rat Anti-Mouse TCF-3/E2A Monoclonal Antibody (Catalog # MAB7650) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to branchial arch nuclei. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3 (Transcription factor 3; also Transcription factor A1 and E2A/alpha plus E12/E47) is a 68-75 kDa nuclear class I member of the bHLH family of molecules. It should not be confused with TCF-3, otherwise known as TCF7-L1, an epidermis-associated beta -catenin regulator. TCF-3 is ubiquitously expressed, and generally serves as a transcriptional regulator. It is able to form covalent homodimers, and following phosphorylation, heterodimers with tissue-specific class II bHLH factors, thus impacting the proliferation and differentiation of select cell types. While heterodimers, and possibly homodimers, activate genes by binding to E boxes, heterodimers involving Id proteins are gene repressors. Mouse TCF-3/E12 is 651 amino acids (aa) in length. It contains one Leu-zipper motif (aa 387-422) plus a bHLH domain (aa 544‑604). TCF-3 contains at least three potential Ser phosphorylation sites. There is one alternative splice form that is termed E47 and shows a 69 aa substitution for aa 527-598. This involves the bHLH domain, and it is believed that each splice form has a distinct function(s). Both splice forms (E12 and E47) do occur simultaneously in the same cell type. Over aa 33-159, mouse TCF-3 shares 94% and 75% aa sequence identity with rat and human TCF-3, respectively.

Long Name
Transcription Factor 3/E2-alpha
Entrez Gene IDs
6929 (Human); 21423 (Mouse); 312451 (Rat)
Alternate Names
bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor

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Citations for Mouse TCF-3/E2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. E2A-PBX1 Remodels Oncogenic Signaling Networks in B-cell Precursor Acute Lymphoid Leukemia
    Authors: Jesús Duque-Afonso, Chiou-Hong Lin, Kyuho Han, Michael C. Wei, Jue Feng, Jason H. Kurzer et al.
    Cancer Research
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Comparative genomics reveals multistep pathogenesis of E2A-PBX1 acute lymphoblastic leukemia.
    Authors: Duque-Afonso J, Feng J, Scherer F, Lin C, Wong S, Wang Z, Iwasaki M, Cleary M
    J Clin Invest, 2015-08-24;125(9):3667-80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. De novo DNA Methyltransferases Dnmt3a and Dnmt3b regulate the onset of Igkappa light chain rearrangement during early B-cell development.
    Authors: Manoharan A, Du Roure C, Rolink A, Matthias P
    Eur J Immunol, 2015-06-16;45(8):2343-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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