Mouse TDRD1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TDRD1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human TDRD1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 739206
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TDRD1
    Asn831-Lys960
    Accession # Q99MV1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TDRD1 in Mouse Testis. TDRD1 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse TDRD1 Monoclonal Antibody (Catalog # MAB6296) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to spermatogonia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TDRD1

TDRD1 (Tudor domain containing protein 1; also MTR-1) is a 130-145 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Mouse TDRD1 is 1172 aa in length. It contains one MYND-type Zn finger region (aa 163-199), and four methyl-binding Tudor domains (aa 307-1032). Over aa 831-960, mouse TDRD1 shares 96% and 66% aa identity with rat and human TDRD1, respectively.

  • Long Name:
    Tudor Domain Containing 1
  • Entrez Gene IDs:
    56165 (Human); 83561 (Mouse); 292129 (Rat)
  • Alternate Names:
    CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; TDRD1; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1
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