Mouse TIMP-1 Quantikine ELISA Kit

(14 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (17 uL), Serum (17 uL), EDTA Plasma (17 uL)
  • Sensitivity
    3.5 pg/mL
  • Assay Range
    37.5 - 2,400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse TIMP-1. This kit detects approximately 80% of the recombinant mouse TIMP-1 complexed with the pro-form of recombinant mouse MMP-9 and approximately 60% when complexed with active recombinant mouse MMP-9.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TIMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse TIMP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TIMP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse TIMP-1.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation9.412.6236.522.141.4


The recovery of mouse TIMP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 102 92-112
EDTA Plasma (n=4) 107 92-119
Serum (n=6) 101 84-117
To assess the linearity of the assay, samples containing high concentrations of mouse TIMP-1 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse TIMP-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIMP-1
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
    • Long Name
      Tissue Inhibitors of Metalloproteinases 1
    • Entrez Gene IDs
      7076 (Human); 21857 (Mouse); 116510 (Rat);
    • Alternate Names
      CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
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    Sample Type
    1. Effects of a high-fat diet on spontaneous metastasis of Lewis lung carcinoma in plasminogen activator inhibitor-1 deficient and wild-type mice.
      Authors: Yan L, DeMars L
      PLoS ONE, 2014;9(10):e110869.
      Species: Mouse
      Sample Type: Plasma
    2. CXCL6 antibody neutralization prevents lung inflammation and fibrosis in mice in the bleomycin model.
      Authors: Besnard A, Struyf S, Guabiraba R, Fauconnier L, Rouxel N, Proost P, Uyttenhove C, Van Snick J, Couillin I, Ryffel B
      J Leukoc Biol, 2013;94(6):1317-23.
      Species: Mouse
      Sample Type: Tissue Homogenates
    3. The effects of low levels of dystrophin on mouse muscle function and pathology.
      Authors: van Putten M, Hulsker M, Nadarajah VD, van Heiningen SH, van Huizen E, van Iterson M, Admiraal P, Messemaker T, den Dunnen JT, 't Hoen PA, Aartsma-Rus A
      PLoS ONE, 2012;7(2):e31937.
      Species: Mouse
      Sample Type: Serum
    4. Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS).
      Authors: Hassan F, Ren D, Zhang W
      PLoS ONE, 2012;7(5):e37912.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    5. Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
      Authors: Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C
      Diabetologia, 2011;54(6):1468-79.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
      Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
      J Neuroinflammation, 2011;8(0):115.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
      Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
      FASEB J., 2010;24(1):70-80.
      Species: Mouse
      Sample Type: BALF
    8. Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.
      Authors: Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM
      PLoS ONE, 2009;4(11):e7916.
      Species: Mouse
      Sample Type: Plasma
    9. Retinoic acid decreases adherence of murine myeloid dendritic cells and increases production of matrix metalloproteinase-9.
      Authors: Lackey DE, Ashley SL, Davis AL, Hoag KA
      J. Nutr., 2008;138(8):1512-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis.
      Authors: Morschl E, Molina JG, Volmer JB, Mohsenin A, Pero RS, Hong JS, Kheradmand F, Lee JJ, Blackburn MR
      Am. J. Respir. Cell Mol. Biol., 2008;39(6):697-705.
      Species: Mouse
      Sample Type: BALF
    11. Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1.
      Authors: Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL
      Am. J. Pathol., 2007;171(6):1762-73.
      Species: Mouse
      Sample Type: Tissue Homogenates
    12. Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model.
      Authors: Madan M, Bishayi B, Hoge M, Amar S
      Atherosclerosis, 2007;197(2):504-14.
      Species: Mouse
      Sample Type: Serum
    13. TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis.
      Authors: Davidsen ML, Wurtz SØ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U
      Br. J. Cancer, 2006;95(8):1114-20.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Effects of bicyclol on dimethylnitrosamine-induced liver fibrosis in mice and its mechanism of action.
      Authors: Hu QW, Liu GT
      Life Sci., 2006;79(6):606-12.
      Species: Mouse
      Sample Type: Serum
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     Mouse TIMP-1 Quantikine ELISA Kit MTM100
      Anonymous 10/06/2016
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     Mouse TIMP-1 Quantikine ELISA Kit MTM100
    : Mouse TIMP-1 Quantikine ELISA Kit [MTM100]


    Sample TestedCell culture supernatant

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