Mouse TL1A/TNFSF15 Antibody Summary
Accession # Q5UBV8
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TL1A/TNFSF15 DuoSet ELISA Kit (Catalog # DY1896-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse TL1A/TNFSF15 ELISA Standard Curve. Recombinant Mouse TL1A/TNFSF15 protein was serially diluted 2-fold and captured by Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB18961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF15. It is expressed predominantly in endothelial cells, and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to DR3/TNFRSF25 on activated T cells.
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