Mouse TL1A/TNFSF15 Antibody

Catalog #: MAB1896 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1896
MAB1896-SP

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Apoptosis Induced by TL1A/ TNFSF15 and Neutralization by Mouse TL1A/TNFSF15 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TL1A/TNFSF15 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TL1A/TNFSF15 in ELISA.

Source

Monoclonal Rat IgG₁ Clone # 820446

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TL1A/TNFSF15
Ile76-Leu252
Accession # Q5UBV8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB18961).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TL1A/TNFSF15 DuoSet ELISA Kit (Catalog # DY1896-05) for convenient development of a sandwich ELISA.

Neutralization

Measured by its ability to neutralize TL1A/TNFSF15-induced apoptosis in the TF‑1 human erythroleukemic cell line. Migone, T. et al. (2002) Immunity 16:479. The Neutralization Dose (ND₅₀) is typically 0.8-4 μg/ml in the presence of 500 ng/mL Recombinant Mouse TL1A/TNFSF15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Apoptosis Induced by TL1A/ TNFSF15 and Neutralization by Mouse TL1A/TNFSF15 Antibody. Recombinant Mouse TL1A/TNFSF15 (Catalog # 1896-TL) induces apoptosis in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Mouse TL1A/TNFSF15 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896). The ND₅₀ is typically 0.8-4 µg/ml.

ELISA

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Mouse TL1A/TNFSF15 ELISA Standard Curve. Recombinant Mouse TL1A/TNFSF15 protein was serially diluted 2-fold and captured by Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB18961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF15. It is expressed predominantly in endothelial cells, and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to DR3/TNFRSF25 on activated T cells.

Long Name

TNF-like 1

Entrez Gene IDs

9966 (Human); 326623 (Mouse)

Alternate Names

MGC129934; MGC129935; TL1A; TL1Vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A; VEGITNF ligand-related molecule 1