Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse TL1A/TNFSF15 Ile76-Leu252 Accession # Q5UBV8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize TL1A/TNFSF15-induced apoptosis in the TF‑1 human erythroleukemic cell line. Migone, T. et al. (2002) Immunity 16:479. The Neutralization Dose (ND50) is typically 0.8-4 μg/ml in the presence of 500 ng/mL Recombinant Mouse TL1A/TNFSF15.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Apoptosis Induced by TL1A/ TNFSF15 and Neutralization by Mouse TL1A/TNFSF15 Antibody. Recombinant Mouse TL1A/TNFSF15 (Catalog # 1896-TL) induces apoptosis in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Mouse TL1A/TNFSF15 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896). The ND50 is typically 0.8-4 μg/ml.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TL1A is a type II transmembrane protein belonging to the TNF
superfamily and has been designated TNFSF15. It is expressed
predominantly in endothelial cells, and its expression is inducible by
TNF-alpha and IL-1 alpha. TL1A binds with high affinity to DR3/TNFRSF25 on
activated T cells.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.