Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 820446
View all TL1A/TNFSF15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TL1A/TNFSF15
Ile76-Leu252
Accession # Q5UBV8

Specificity

Detects mouse TL1A/TNFSF15 in ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse TL1A/TNFSF15 Antibody

Apoptosis Induced by TL1A/ TNFSF15 and Neutralization by Mouse TL1A/TNFSF15 Antibody.

Apoptosis Induced by TL1A/ TNFSF15 and Neutralization by Mouse TL1A/TNFSF15 Antibody.

Recombinant Mouse TL1A/TNFSF15 (Catalog # 1896-TL) induces apoptosis in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Mouse TL1A/TNFSF15 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896). The ND50 is typically 0.8-4 µg/ml.
Mouse TL1A/TNFSF15 Antibody in ELISA Standard Curve.

Mouse TL1A/TNFSF15 ELISA Standard Curve.

Recombinant Mouse TL1A/TNFSF15 protein was serially diluted 2-fold and captured by Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB18961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB1896) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Mouse TL1A/TNFSF15 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB18961).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TL1A/TNFSF15 DuoSet ELISA Kit (Catalog # DY1896-05) for convenient development of a sandwich ELISA.

Neutralization

Measured by its ability to neutralize TL1A/TNFSF15-induced apoptosis in the TF‑1 human erythroleukemic cell line. Migone, T. et al. (2002) Immunity 16:479. The Neutralization Dose (ND50) is typically 0.8-4 μg/ml in the presence of 500 ng/mL Recombinant Mouse TL1A/TNFSF15.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF15. It is expressed predominantly in endothelial cells, and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to DR3/TNFRSF25 on activated T cells.

Long Name

TNF-like 1

Alternate Names

TNFSF15, VEGI

Entrez Gene IDs

9966 (Human); 326623 (Mouse)

Gene Symbol

TNFSF15

UniProt

Q5UBV8

Additional TL1A/TNFSF15 Products

Product Documents for Mouse TL1A/TNFSF15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TL1A/TNFSF15 Antibody

For research use only

Citations for Mouse TL1A/TNFSF15 Antibody

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