Mouse TLR6 Antibody

Catalog # Availability Size / Price Qty
MAB1533
MAB1533-SP

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Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (1)
FAQs
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Reviews (1)

Mouse TLR6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR6. Stains mouse TLR6 transfectants and not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 418601
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse TLR6
Phe39-Thr806
Accession # BAA78632
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/ macrophage cell line was stained with Rat Anti-Mouse TLR6 Monoclonal Antibody (Catalog # MAB1533, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR6

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

References
  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Roeder, A. et al. (2004) Med. Mycol. 42:485.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Wetzler, L.M. (2003) Vaccine 21:S55.
  5. Takeuchi, O. et al. (1999) Gene 231:59.
  6. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
  7. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
  8. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  10. Bulut, Y. et al. (2001) J. Immunol. 167:987.
  11. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
  12. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
  13. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  14. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
  15. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
Long Name
Toll-like Receptor 6
Entrez Gene IDs
10333 (Human); 21899 (Mouse); 305353 (Rat)
Alternate Names
CD286 antigen; CD286; TLR6; toll-like receptor 6

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Citation for Mouse TLR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lipid raft localization of TLR2 and its co-receptors is independent of membrane lipid composition
    Authors: C Hellwing, A Schoeniger, C Roessler, A Leimert, J Schumann
    PeerJ, 2018;6(0):e4212.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Mouse TLR6 Antibody
By Celine Beamer on 03/18/2016
Application: Flow Sample Tested: alveolar macrophages,Bone marrow-derived macrophages Species: Mouse

Acute silica exposure reduced TLR 6 expression on F4-80+CD11c+ alveolar macrophages. C57Bl/6 wild-type mice were exposed to saline (25 μl, black line) or silica (1 mg, gray line) through intranasal aspiration. After 4 h, whole lungs were lavaged, cells immunostained, and surface markers assessed by flow symmetry using a BD FACS Aria II. Data shown as histograms relative to unstained control (dashed line).