Mouse TLR6 Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TLR6. Stains mouse TLR6 transfectants and not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 418601
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with mouse TLR6
    Phe39-Thr806
    Accession # BAA78632
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/
macrophage cell line was stained with Rat Anti-Mouse TLR6 Monoclonal Antibody (Catalog # MAB1533, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR6

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

  • References:
    1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    2. Roeder, A. et al. (2004) Med. Mycol. 42:485.
    3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
    4. Wetzler, L.M. (2003) Vaccine 21:S55.
    5. Takeuchi, O. et al. (1999) Gene 231:59.
    6. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
    7. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
    8. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
    9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
    10. Bulut, Y. et al. (2001) J. Immunol. 167:987.
    11. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
    12. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
    13. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
    14. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
    15. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
  • Long Name:
    Toll-like Receptor 6
  • Entrez Gene IDs:
    10333 (Human); 21899 (Mouse); 305353 (Rat)
  • Alternate Names:
    CD286 antigen; CD286; TLR6; toll-like receptor 6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse TLR6 Antibody MAB1533
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 Flow Mouse Anonymous 03/18/2016
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Flow Cytometry Mouse TLR6 Antibody MAB1533
Flow Cytometry: Mouse TLR6 Antibody [MAB1533]

Summary

ApplicationFlow Cytometry
Sample TestedBone marrow-derived macrophages,alveolar macrophages
SpeciesMouse

Other Experimental Details

Other Experimental DetailsAcute silica exposure reduced TLR 6 expression on F4-80+CD11c+ alveolar macrophages. C57Bl/6 wild-type mice were exposed to saline (25 μl, black line) or silica (1 mg, gray line) through intranasal aspiration. After 4 h, whole lungs were lavaged, cells immunostained, and surface markers assessed by flow symmetry using a BD FACS Aria II. Data shown as histograms relative to unstained control (dashed line).

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