Mouse TM4SF1 Antibody

Detection of Mouse TM4SF1 by Western Blot. Western blot shows lysates of LL/2 mouse Lewis lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TM4SF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7514) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TM4SF1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TM4SF1 in Mouse Embryo. TM4SF1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse TM4SF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7514) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the trabeculae of the developing heart. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Human TM4SF1 by Western Blot Laminin promotes the interaction between TM4SF1 and integrin alpha 6 in Eca109 cells.A Con- and TM4SF1 overexpressed- Eca109 cell lysates (under laminin-coating condition) were immunoblotted by anti-p-FAK, anti-FAK, anti-p-PI3K p85 (Try458), anti- PI3K p85, anti-pAKT, and anti-AKT antibodies. B List of TM4SF1-associated cell membrane proteins in Eca109 cells under laminin-coating condition, identified by mass spectrometric analysis. C Co-IP of endogenous TM4SF1 with endogenous integrin alpha 6, beta 4, and beta 1 in cell lysate. TM4SF1 was immunoprecipitated from Eca109 cells, which were cultured on the dishes with or without laminin-coating, and immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, and TM4SF1. The input was immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, p-FAK, FAK, TM4SF1, and beta -actin. D Co-IP of endogenous TM4SF1 with endogenous integrin alpha 6, beta 4, and beta 1 in ESCC patient tissues. TM4SF1 was immunoprecipitated from homogenized ESCC patient tissues lysate and immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, and TM4SF1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35835740), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TM4SF1 by Western Blot The Laminin-TM4SF1-integrin alpha 6-FAK signaling axis mediates ESCC cell migration. A–C Immunoblotting of p-FAK, FAK, TM4SF1, integrin alpha 6, and beta -actin in (left panel) and migration ability of (right panel) TM4SF1-overexpressed, integrin alpha 6-overexpressed, and TM4SF1/integrin alpha 6 double overexpressed Eca109 cells (A), Con-, TM4SF1-knockdown-, related TM4SF1 rescued-, integrin alpha 6- overexpressed, and TM4SF1-rescued/integrin alpha 6- overexpressed KYSE-410 cells (B), and integrin alpha 6-knockdown- and related alpha 6 rescued- Con or TM4SF1 overexpressed Eca109 cells (C) under laminin coating condition (LE: long exposure; SE: short exposure). Representative photos were taken, and then the migrated cells were counted. Statistical significance was determined by a two-tailed unpaired t test. Error bars are means ± s.d. (right panel; n = 3; *P < 0.05; **P < 0.01; ***P < 0.001). D Immunoblotting of p-FAK, FAK, TM4SF1, integrin alpha 6, and beta -actin in (upper panel) and migration ability of (lower panel) TM4SF1-overexpressed, integrin alpha 6-overexpressed, and TM4SF1/integrin alpha 6 double overexpressed KYSE-410 cells pre-treated with or without VS-4718 (1 μM) for 24 h. Representative photos were taken and then the migrated cells were counted. Statistical significance was determined by a two-tailed unpaired t test. Error bars are means ± s.d. (right panel; n = 3; n.s., not statistically significant; *P < 0.05; ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35835740), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TM4SF1 by Western Blot Laminin promotes the interaction between TM4SF1 and integrin alpha 6 in Eca109 cells.A Con- and TM4SF1 overexpressed- Eca109 cell lysates (under laminin-coating condition) were immunoblotted by anti-p-FAK, anti-FAK, anti-p-PI3K p85 (Try458), anti- PI3K p85, anti-pAKT, and anti-AKT antibodies. B List of TM4SF1-associated cell membrane proteins in Eca109 cells under laminin-coating condition, identified by mass spectrometric analysis. C Co-IP of endogenous TM4SF1 with endogenous integrin alpha 6, beta 4, and beta 1 in cell lysate. TM4SF1 was immunoprecipitated from Eca109 cells, which were cultured on the dishes with or without laminin-coating, and immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, and TM4SF1. The input was immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, p-FAK, FAK, TM4SF1, and beta -actin. D Co-IP of endogenous TM4SF1 with endogenous integrin alpha 6, beta 4, and beta 1 in ESCC patient tissues. TM4SF1 was immunoprecipitated from homogenized ESCC patient tissues lysate and immunoblotted with antibodies against integrin alpha 6, beta 4, beta 1, and TM4SF1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35835740), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TM4SF1 by Western Blot The Laminin-TM4SF1-integrin alpha 6-FAK signaling axis mediates ESCC cell migration. A–C Immunoblotting of p-FAK, FAK, TM4SF1, integrin alpha 6, and beta -actin in (left panel) and migration ability of (right panel) TM4SF1-overexpressed, integrin alpha 6-overexpressed, and TM4SF1/integrin alpha 6 double overexpressed Eca109 cells (A), Con-, TM4SF1-knockdown-, related TM4SF1 rescued-, integrin alpha 6- overexpressed, and TM4SF1-rescued/integrin alpha 6- overexpressed KYSE-410 cells (B), and integrin alpha 6-knockdown- and related alpha 6 rescued- Con or TM4SF1 overexpressed Eca109 cells (C) under laminin coating condition (LE: long exposure; SE: short exposure). Representative photos were taken, and then the migrated cells were counted. Statistical significance was determined by a two-tailed unpaired t test. Error bars are means ± s.d. (right panel; n = 3; *P < 0.05; **P < 0.01; ***P < 0.001). D Immunoblotting of p-FAK, FAK, TM4SF1, integrin alpha 6, and beta -actin in (upper panel) and migration ability of (lower panel) TM4SF1-overexpressed, integrin alpha 6-overexpressed, and TM4SF1/integrin alpha 6 double overexpressed KYSE-410 cells pre-treated with or without VS-4718 (1 μM) for 24 h. Representative photos were taken and then the migrated cells were counted. Statistical significance was determined by a two-tailed unpaired t test. Error bars are means ± s.d. (right panel; n = 3; n.s., not statistically significant; *P < 0.05; ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35835740), licensed under a CC-BY license. Not internally tested by R&D Systems.