Mouse TREM-2 Biotinylated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TREM‑2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TREM-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TREM-2b
    Leu19-Pro168
    Accession # Q99NH8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TREM‑2b Fc Chimera (Catalog # 1729-T2)
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TREM‑2 in Mouse Splenocytes.

TREM‑2 was detected in immersion fixed mouse spleno­cytes using Mouse Anti-TREM‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-2

TREM-2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM‑2a and TREM‑2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM‑2 is expressed by immature monocyte‑derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and co‑stimulatory signals (4). Ligation of TREM‑2 on immature DC with anti-TREM‑2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM‑2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM‑2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM‑2 deficient individuals (6).

  • References:
    1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
    2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
    3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
    4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
    5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
    6. Cella, M. et al. (2003) J. Exp. Med. 198:645.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 2
  • Entrez Gene IDs:
    54209 (Human)
  • Alternate Names:
    TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia.
    Authors: Sharif O, Gawish R, Warszawska J, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher R, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S
    PLoS Pathog, 2014;10(6):e1004167.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2.
    Authors: Koth LL, Cambier CJ, Ellwanger A
    J. Immunol., 2010;184(11):6522-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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