Mouse TREM2 Antibody
Mouse TREM2 Antibody Summary
Leu19-Pro168
Accession # Q99NH8
Applications
This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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TREM2 in RAW 264.7 Mouse Cell Line. TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
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TREM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (left panel) but is not detected in TREM2 knockout (KO) RAW 264.7 Mouse Cell Line cell line (right panel) using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM2
TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals (6).
- Colonna, M. (2003) Nature Rev. Immunol. 3:445.
- Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
- Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
- Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
- Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
- Cella, M. et al. (2003) J. Exp. Med. 198:645.
Product Datasheets
Citations for Mouse TREM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Muramyl dipeptide-mediated immunomodulation on monocyte subsets exerts therapeutic effects in a mouse model of Alzheimer's disease
Authors: A Fani Malek, G Cisbani, MM Plante, P Préfontain, N Laflamme, J Gosselin, S Rivest
J Neuroinflammation, 2020;17(1):218.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice
Authors: VS Jadhav, PBC Lin, T Pennington, GV Di Prisco, AJ Jannu, G Xu, M Moutinho, J Zhang, BK Atwood, SS Puntambeka, SJ Bissel, AL Oblak, GE Landreth, BT Lamb
Mol Neurodegener, 2020;15(1):62.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice
Authors: Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang
Aging (Albany NY), 2020;12(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Peroxiredoxin 6 mediates protective function of astrocytes in A&beta proteostasis
Authors: JE Pankiewicz, JR Diaz, M Martá-Ariz, AM Lizi?czyk, LA Franco, MJ Sadowski
Mol Neurodegener, 2020;15(1):50.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
APOE genotype and sex affect microglial interactions with plaques in Alzheimer's disease mice
Authors: TL Stephen, M Cacciottol, D Balu, TE Morgan, MJ LaDu, CE Finch, CJ Pike
Acta Neuropathol Commun, 2019;7(1):82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease
Authors: PJ Cheng-Hath, EG Reed-Geagh, TR Jay, BT Casali, SM Bemiller, SS Puntambeka, VE von Saucke, RY Williams, JC Karlo, M Moutinho, G Xu, RM Ransohoff, BT Lamb, GE Landreth
Mol Neurodegener, 2018;13(1):29.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
Neuron, 2018;97(5):1023-1031.e7.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models
Authors: CYD Lee, A Daggett, X Gu, LL Jiang, P Langfelder, X Li, N Wang, Y Zhao, CS Park, Y Cooper, I Ferando, I Mody, G Coppola, H Xu, XW Yang
Neuron, 2018;97(5):1032-1048.e5.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Intracellular trafficking of TREM2 is regulated by presenilin 1
Authors: Y Zhao, X Li, T Huang, LL Jiang, Z Tan, M Zhang, IH Cheng, X Wang, G Bu, YW Zhang, Q Wang, H Xu
Exp. Mol. Med., 2017;49(12):e405.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury
Authors: EL Castranio, A Mounier, CM Wolfe, KN Nam, NF Fitz, F Letronne, J Schug, R Koldamova, I Lefterov
Neurobiol. Dis., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice
Authors: KN Nam, A Mounier, CM Wolfe, NF Fitz, AY Carter, EL Castranio, HI Kamboh, VL Reeves, J Wang, X Han, J Schug, I Lefterov, R Koldamova
Sci Rep, 2017;7(1):4307.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology
Authors: Z Zhang, Y Takeda-Uch, T Foyez, S Ohtake-Nii, Narentuya, H Akatsu, K Nishitsuji, M Michikawa, T Wyss-Coray, K Kadomatsu, K Uchimura
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
MicroRNA-101a regulates microglial morphology and inflammation
Authors: R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake
J Neuroinflammation, 2017;14(1):109.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Differential modulation of TREM2 protein during postnatal brain development in mice.
Authors: Chertoff M, Shrivastava K, Gonzalez B, Acarin L, Gimenez-Llort L
PLoS ONE, 2013;8(8):e72083.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
In Situ Dividing and Phagocytosing Retinal Microglia Express Nestin, Vimentin, and NG2 In Vivo.
Authors: Wohl SG, Schmeer CW, Friese T, Witte OW, Isenmann S
PLoS ONE, 2011;6(8):e22408.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer's disease.
Authors: Pons V, Levesque P, Plante M, Rivest S
Alzheimers Res Ther, 0;13(1):8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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Only works for IF staining in cells
30 and 60 micrograms loaded for each experiment. We tried AB concentratrations from 1:10.000 to 1:1000, none of them working
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