Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Knockout Validated, Western Blot, ELISA, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Co-Immunoprecipitation, ELISA Capture, IF/ICC, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TREM2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM2b
Leu19-Pro168
Accession # Q99NH8

Specificity

Detects mouse TREM2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse TREM2 Antibody

TREM2 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

TREM2 in RAW 264.7 Mouse Cell Line.

TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse TREM2 Antibody in ELISA Standard Curve.

Mouse TREM2 ELISA Standard Curve.

Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

TREM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (left panel) but is not detected in TREM2 knockout (KO) RAW 264.7 Mouse Cell Line cell line (right panel) using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

SCF+G-CSF treatment increases TREM2 expression in the Iba1+ microglia/macrophages surrounding the 6E10+ senile plaques. (A) Representative confocal images of TREM2 (red), 6E10 (purple) and Iba1 (green) triple immunofluorescence staining in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) illustrates the location and interaction of TREM2 + cells (red) and 6E10+ A beta plaques (white) in the brains of aged APP/PS1 mice. (C) Quantification data show the percentage of TREM2+ area surrounding the 6E10+ A beta plaques (within 10μm from the border of the A beta plaques) in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (D) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) displays the location and interaction of TREM2+/Iba1+ co-expressing cells (yellow) and 6E10+ A beta plaques (white) in the brains of APP/PS1 mice. (E) Quantification data show the percentage of TREM2+/Iba1+ co-expression area in the total of Iba1+ area in the vicinity of 6E10+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. N=4-5. Mean ± SEM. * p<0.05 by Student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

SCF+G-CSF treatment increases TREM2 expression in the Iba1+ microglia/macrophages surrounding the 6E10+ senile plaques. (A) Representative confocal images of TREM2 (red), 6E10 (purple) and Iba1 (green) triple immunofluorescence staining in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) illustrates the location and interaction of TREM2 + cells (red) and 6E10+ A beta plaques (white) in the brains of aged APP/PS1 mice. (C) Quantification data show the percentage of TREM2+ area surrounding the 6E10+ A beta plaques (within 10μm from the border of the A beta plaques) in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (D) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) displays the location and interaction of TREM2+/Iba1+ co-expressing cells (yellow) and 6E10+ A beta plaques (white) in the brains of APP/PS1 mice. (E) Quantification data show the percentage of TREM2+/Iba1+ co-expression area in the total of Iba1+ area in the vicinity of 6E10+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. N=4-5. Mean ± SEM. * p<0.05 by Student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

Detection of Mouse TREM2 by Immunocytochemistry/Immunofluorescence

SCF+G-CSF treatment increases TREM2 expression in the Iba1+ microglia/macrophages surrounding the 6E10+ senile plaques. (A) Representative confocal images of TREM2 (red), 6E10 (purple) and Iba1 (green) triple immunofluorescence staining in the brains of aged APP/PS1 mice. Blue: Nuclear counterstaining by DAPI. (B) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) illustrates the location and interaction of TREM2 + cells (red) and 6E10+ A beta plaques (white) in the brains of aged APP/PS1 mice. (C) Quantification data show the percentage of TREM2+ area surrounding the 6E10+ A beta plaques (within 10μm from the border of the A beta plaques) in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. (D) Representative orthographic view of z-stack images (12 z-stacks with 1μm intervals) displays the location and interaction of TREM2+/Iba1+ co-expressing cells (yellow) and 6E10+ A beta plaques (white) in the brains of APP/PS1 mice. (E) Quantification data show the percentage of TREM2+/Iba1+ co-expression area in the total of Iba1+ area in the vicinity of 6E10+ A beta plaques in the brains of aged APP/PS1 mice with or without SCF+G-CSF treatment. N=4-5. Mean ± SEM. * p<0.05 by Student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33269098), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse TREM2 Antibody by Western Blot

Detection of Mouse Mouse TREM2 Antibody by Western Blot

A beta oligomers induce TREM2 proteolysis and sTREM2 release, which then binds A beta oligomers, but R47H sTREM2 binds less. A, western blot of cell lysate and unprocessed supernatant (sTREM2) of HEK293 cells coexpressing human DAP12 and full-length N-terminally-tagged wild-type (WT) human TREM2 (FL-TREM2) 16 h after adding A beta oligomers. This blot and those for A beta monomers and fibrils are reproduced in Figure S5 for comparison. B, quantification of sTREM2 release from transfected HEK293 cells expressing wild-type (green line) and R47H TREM2 (red line). C, quantification of sTREM2 release from wild-type TREM2 expressing HEK293 cells induced by doses of A beta oligomers (red line), monomers (green line), or fibrils (blue line). For both (B and C) error bars = SEM; ∗p < 0.05 ∗∗p < 0.01 ∗∗∗p < 0.001, n = 3 independent experiments; one-way ANOVA with Tukey's post-hoc multiple comparisons test. D, example field of single-molecule TIRF imaging of mixture of A beta oligomers (green) and wild-type TREM2 ectodomain (red), where colocalized spots appear yellow. Scale bar: 1 micron. Magnified image of three sections of field at right. E, proportion of monomeric or oligomeric A beta colocalized with wild-type sTREM2. F, proportion of A beta oligomers colocalized with wild-type or R47H TREM2 ectodomain. For (E and F), error bars = SEM; ∗∗∗∗p < 0.0001, n = 3 independent preparations, each analyzed in nine fields each; two-tailed t-test of significance. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33823153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TREM2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Knockout Validated

1.7-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line 

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TREM2b Fc Chimera (Catalog # 1729-T2)

Reviewed Applications

Read 5 reviews rated 3.4 using AF1729 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM2

TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals (6).

References

  1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
  2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
  3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
  4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
  5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
  6. Cella, M. et al. (2003) J. Exp. Med. 198:645.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

Q99NH8 (Mouse)

Additional TREM2 Products

Product Documents for Mouse TREM2 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TREM2 Antibody

For research use only

Citations for Mouse TREM2 Antibody

Customer Reviews for Mouse TREM2 Antibody (5)

3.4 out of 5
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Showing  1 - 5 of 5 reviews Showing All
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  • Mouse TREM2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain (cortex) tissue
    Species: Mouse
    Verified Customer | Posted 06/07/2023
    Mouse TREM2 Antibody AF1729
  • Mouse TREM2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: RAW 264.7 Mouse Cell Line
    Species: Mouse
    Verified Customer | Posted 07/11/2022
    Mouse TREM2 Antibody AF1729
  • Mouse TREM2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: kupffer cells
    Species: Mouse
    Verified Customer | Posted 09/28/2020
    Only works for IF staining in cells
    Mouse TREM2 Antibody AF1729
  • Mouse TREM-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry of free floating tissue
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 03/20/2018
    1:250 dilution, antigen retrieval for 15mins.
    Mouse TREM2 Antibody AF1729
  • Mouse TREM-2 Antibody
    Name: Dennis Berner
    Application: Western Blot
    Sample Tested: Brain tissue and Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 07/03/2017
    30 and 60 micrograms loaded for each experiment. We tried AB concentratrations from 1:10.000 to 1:1000, none of them working
    Bio-Techne Response
    Technical Support is following up

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