Mouse TREM2 Antibody

Catalog # Availability Size / Price Qty
AF1729
AF1729-SP
Mouse TREM2 Antibody in Immunocytochemistry
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)

Mouse TREM2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TREM-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-2b
Leu19-Pro168
Accession # Q99NH8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TREM‑2b Fc Chimera (Catalog # 1729-T2)
ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunocytochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry TREM2  in RAW 264.7 Mouse Cell Line. View Larger

TREM2 in RAW 264.7 Mouse Cell Line. TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM-2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM‑2a and TREM‑2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM‑2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM‑2 on immature DC with anti-TREM‑2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM‑2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM‑2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM‑2 deficient individuals (6).

References
  1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
  2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
  3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
  4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
  5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
  6. Cella, M. et al. (2003) J. Exp. Med. 198:645.
Long Name
Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs
54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2

Product Datasheets

Citations for Mouse TREM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. APOE genotype and sex affect microglial interactions with plaques in Alzheimer's disease mice
    Authors: TL Stephen, M Cacciottol, D Balu, TE Morgan, MJ LaDu, CE Finch, CJ Pike
    Acta Neuropathol Commun, 2019;7(1):82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease
    Authors: PJ Cheng-Hath, EG Reed-Geagh, TR Jay, BT Casali, SM Bemiller, SS Puntambeka, VE von Saucke, RY Williams, JC Karlo, M Moutinho, G Xu, RM Ransohoff, BT Lamb, GE Landreth
    Mol Neurodegener, 2018;13(1):29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
    Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
    Neuron, 2018;97(5):1023-1031.e7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models
    Authors: CYD Lee, A Daggett, X Gu, LL Jiang, P Langfelder, X Li, N Wang, Y Zhao, CS Park, Y Cooper, I Ferando, I Mody, G Coppola, H Xu, XW Yang
    Neuron, 2018;97(5):1032-1048.e5.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Intracellular trafficking of TREM2 is regulated by presenilin 1
    Authors: Y Zhao, X Li, T Huang, LL Jiang, Z Tan, M Zhang, IH Cheng, X Wang, G Bu, YW Zhang, Q Wang, H Xu
    Exp. Mol. Med., 2017;49(12):e405.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  6. Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury
    Authors: EL Castranio, A Mounier, CM Wolfe, KN Nam, NF Fitz, F Letronne, J Schug, R Koldamova, I Lefterov
    Neurobiol. Dis., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice
    Authors: KN Nam, A Mounier, CM Wolfe, NF Fitz, AY Carter, EL Castranio, HI Kamboh, VL Reeves, J Wang, X Han, J Schug, I Lefterov, R Koldamova
    Sci Rep, 2017;7(1):4307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology
    Authors: Z Zhang, Y Takeda-Uch, T Foyez, S Ohtake-Nii, Narentuya, H Akatsu, K Nishitsuji, M Michikawa, T Wyss-Coray, K Kadomatsu, K Uchimura
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. MicroRNA-101a regulates microglial morphology and inflammation
    Authors: R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake
    J Neuroinflammation, 2017;14(1):109.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Differential modulation of TREM2 protein during postnatal brain development in mice.
    Authors: Chertoff M, Shrivastava K, Gonzalez B, Acarin L, Gimenez-Llort L
    PLoS ONE, 2013;8(8):e72083.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. In Situ Dividing and Phagocytosing Retinal Microglia Express Nestin, Vimentin, and NG2 In Vivo.
    Authors: Wohl SG, Schmeer CW, Friese T, Witte OW, Isenmann S
    PLoS ONE, 2011;6(8):e22408.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse TREM-2 Antibody
By Anonymous on 07/03/2017
Application: WB Sample Tested: Brain tissue,Bone marrow cells Species: Mouse

30 and 60 micrograms loaded for each experiment. We tried AB concentratrations from 1:10.000 to 1:1000, none of them working

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