Mouse TREM2 Antibody
Mouse TREM2 Antibody Summary
Leu19-Pro168
Accession # Q99NH8
Applications
This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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TREM2 in RAW 264.7 Mouse Cell Line. TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM2
TREM-2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM‑2a and TREM‑2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM‑2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM‑2 on immature DC with anti-TREM‑2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM‑2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM‑2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM‑2 deficient individuals (6).
- Colonna, M. (2003) Nature Rev. Immunol. 3:445.
- Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
- Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
- Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
- Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
- Cella, M. et al. (2003) J. Exp. Med. 198:645.
Product Datasheets
Citations for Mouse TREM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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APOE genotype and sex affect microglial interactions with plaques in Alzheimer's disease mice
Authors: TL Stephen, M Cacciottol, D Balu, TE Morgan, MJ LaDu, CE Finch, CJ Pike
Acta Neuropathol Commun, 2019;7(1):82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease
Authors: PJ Cheng-Hath, EG Reed-Geagh, TR Jay, BT Casali, SM Bemiller, SS Puntambeka, VE von Saucke, RY Williams, JC Karlo, M Moutinho, G Xu, RM Ransohoff, BT Lamb, GE Landreth
Mol Neurodegener, 2018;13(1):29.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
Neuron, 2018;97(5):1023-1031.e7.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models
Authors: CYD Lee, A Daggett, X Gu, LL Jiang, P Langfelder, X Li, N Wang, Y Zhao, CS Park, Y Cooper, I Ferando, I Mody, G Coppola, H Xu, XW Yang
Neuron, 2018;97(5):1032-1048.e5.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Intracellular trafficking of TREM2 is regulated by presenilin 1
Authors: Y Zhao, X Li, T Huang, LL Jiang, Z Tan, M Zhang, IH Cheng, X Wang, G Bu, YW Zhang, Q Wang, H Xu
Exp. Mol. Med., 2017;49(12):e405.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury
Authors: EL Castranio, A Mounier, CM Wolfe, KN Nam, NF Fitz, F Letronne, J Schug, R Koldamova, I Lefterov
Neurobiol. Dis., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice
Authors: KN Nam, A Mounier, CM Wolfe, NF Fitz, AY Carter, EL Castranio, HI Kamboh, VL Reeves, J Wang, X Han, J Schug, I Lefterov, R Koldamova
Sci Rep, 2017;7(1):4307.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology
Authors: Z Zhang, Y Takeda-Uch, T Foyez, S Ohtake-Nii, Narentuya, H Akatsu, K Nishitsuji, M Michikawa, T Wyss-Coray, K Kadomatsu, K Uchimura
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
MicroRNA-101a regulates microglial morphology and inflammation
Authors: R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake
J Neuroinflammation, 2017;14(1):109.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Differential modulation of TREM2 protein during postnatal brain development in mice.
Authors: Chertoff M, Shrivastava K, Gonzalez B, Acarin L, Gimenez-Llort L
PLoS ONE, 2013;8(8):e72083.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
In Situ Dividing and Phagocytosing Retinal Microglia Express Nestin, Vimentin, and NG2 In Vivo.
Authors: Wohl SG, Schmeer CW, Friese T, Witte OW, Isenmann S
PLoS ONE, 2011;6(8):e22408.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr
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30 and 60 micrograms loaded for each experiment. We tried AB concentratrations from 1:10.000 to 1:1000, none of them working
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