Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody

Catalog # Availability Size / Price Qty
MAB9185-100
MAB9185-SP
Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody in Data
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Product Details
Citations (1)
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Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse uPA in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 901420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPA
Met1-Phe433
Accession # P06869
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of u‑Plasminogen Activator (uPA)/Urokinase by Western Blot. View Larger

Detection of u‑Plasminogen Activator (uPA)/Urokinase by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry u‑Plasminogen Activator (uPA)/Urokinase in Mouse Kidney. View Larger

u‑Plasminogen Activator (uPA)/Urokinase in Mouse Kidney. u-Plasminogen Activator (uPA)/Urokinase was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: u-Plasminogen Activator (uPA)/Urokinase

Urokinase-type plasminogen activator (uPA) is a 378 amino acid enzyme which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPa occurs in high and low molecular mass forms each consisting of two chains (A and B).  The high mass form has a long A chain while the low mass form has a short chain AuPA is widely expressed in normal tissues including epithelia, muscle, reproductive organs and brain, and  is highly expressed in kidney, where it localizes to proximal and distal tubules and is secreted into urine. uPA is known to play a role in wound healing and  tissue remodeling and  tumor invasion

Long Name
Urokinase-type Plasminogen Activator
Entrez Gene IDs
5328 (Human); 18792 (Mouse)
Alternate Names
ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator

Product Datasheets

Citation for Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Pirfenidone inhibits motility of NSCLC cells by interfering with the urokinase system
    Authors: M Krämer, P Markart, F Drakopanag, A Mamazhakyp, L Schaefer, M Didiasova, M Wygrecka
    Cell. Signal., 2019;65(0):109432.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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