Detection of u‑Plasminogen Activator (uPA)/Urokinase by Western Blot.
Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u‑Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u‑Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
u‑Plasminogen Activator (uPA)/Urokinase in Mouse Kidney.
u‑Plasminogen Activator (uPA)/Urokinase was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse u‑Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Urokinase-type plasminogen activator (uPA) is a 378
amino acid enzyme which cleaves the zymogen plasminogen to form the active enzyme
plasmin. uPa occurs in high and low molecular mass forms each consisting of two
chains (A and B). The high mass form has
a long A chain while the low mass form has a short chain AuPA is widely
expressed in normal tissues including epithelia, muscle, reproductive organs
and brain, and is highly expressed in
kidney, where it localizes to proximal and distal tubules and is secreted into
urine. uPA is known to play a role in wound healing and tissue remodeling and tumor invasion
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