Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody MAB9185-100: R&D Systems

Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse uPA in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 901420
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse uPA
    Met1-Phe433
    Accession # P06869
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of u‑Plasminogen Activator (uPA)/Urokinase by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u‑Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u‑Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
u‑Plasminogen Activator (uPA)/Urokinase in Mouse Kidney.
u‑Plasminogen Activator (uPA)/Urokinase was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse u‑Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: u-Plasminogen Activator (uPA)/Urokinase
Urokinase-type plasminogen activator (uPA) is a 378 amino acid enzyme which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPa occurs in high and low molecular mass forms each consisting of two chains (A and B).  The high mass form has a long A chain while the low mass form has a short chain AuPA is widely expressed in normal tissues including epithelia, muscle, reproductive organs and brain, and  is highly expressed in kidney, where it localizes to proximal and distal tubules and is secreted into urine. uPA is known to play a role in wound healing and  tissue remodeling and  tumor invasion
  • Long Name:
    Urokinase-type Plasminogen Activator
  • Entrez Gene IDs:
    5328 (Human); 18792 (Mouse)
  • Alternate Names:
    ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator
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