Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody Summary
Accession # P06869
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of u‑Plasminogen Activator (uPA)/Urokinase by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
u‑Plasminogen Activator (uPA)/Urokinase in Mouse Kidney. u-Plasminogen Activator (uPA)/Urokinase was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: u-Plasminogen Activator (uPA)/Urokinase
Urokinase-type plasminogen activator (uPA) is a 378 amino acid enzyme which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPa occurs in high and low molecular mass forms each consisting of two chains (A and B). The high mass form has a long A chain while the low mass form has a short chain AuPA is widely expressed in normal tissues including epithelia, muscle, reproductive organs and brain, and is highly expressed in kidney, where it localizes to proximal and distal tubules and is secreted into urine. uPA is known to play a role in wound healing and tissue remodeling and tumor invasion
Citation for Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Pirfenidone inhibits motility of NSCLC cells by interfering with the urokinase system
Authors: M Krämer, P Markart, F Drakopanag, A Mamazhakyp, L Schaefer, M Didiasova, M Wygrecka
Cell. Signal., 2019;65(0):109432.
Sample Types: Cell Lysates
Applications: Western Blot
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