Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 774056
View all Uromodulin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Uromodulin
Ser24-Ala618
Accession # Q91X17

Specificity

Detects mouse Uromodulin in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Uromodulin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Uromodulin Antibody

Detection of Mouse Uromodulin antibody by Western Blot.

Detection of Mouse Uromodulin by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Uromodulin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

Uromodulin in Mouse Kidney.

Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Uromodulin by Western Blot

Detection of Uromodulin by Western Blot

Tolvaptan induces Nrf2 nuclear translocation and HO-1 expression in vivo. (a) Tolvaptan promotes Nrf2 nuclear translocation in the outer medulla of mouse kidneys. (Top panel) Male C57BL/6J mice aged 8–9-weeks were fed with a diet containing 0.5% tolvaptan for 24 h. The nuclear extract was obtained from the outer medulla. Arrow indicates the band of Nrf2. (Bottom panel) Nrf2 bands were quantified using densitometric analysis. Bar indicates average from four or five experiments (control: n = 4, tolvaptan: n = 5). Student’s t-test, **P < 0.01. C: control, Tol: 0.5% tolvaptan. (b) Tolvaptan increases HO-1 protein expression in the outer medulla of mouse kidneys. (Top panel) Tolvaptan (0.5%) was administered via diet to 8–9-week-old male C57BL/6J mice for 24 h. The kidneys were separated into the cortex and the outer medulla, and the whole protein extraction was performed. The blots of HO-1 and Actin were configured with the blot of cortex and that of outer medulla. Fractionation of the cortex and the outer medulla was verified using UMOD and pNCC antibodies. (Bottom panel) Densitometric analysis of HO-1 is shown. Bar indicates average from four or five experiments (control: n = 4, tolvaptan: n = 5). Student’s t-test, *P < 0.05. C: control, Tol: 0.5% tolvaptan. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31239473), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Uromodulin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse kidney

Western Blot

0.5 µg/mL
Sample: Mouse kidney tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB5175 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Uromodulin

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.

Alternate Names

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid

Entrez Gene IDs

7369 (Human); 22242 (Mouse); 25128 (Rat)

Gene Symbol

UMOD

UniProt

Q91X17

Additional Uromodulin Products

Product Documents for Mouse Uromodulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Uromodulin Antibody

For research use only

Citations for Mouse Uromodulin Antibody

Customer Reviews for Mouse Uromodulin Antibody (2)

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  • Mouse Uromodulin Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 10/25/2021
    Mouse Uromodulin Antibody MAB5175
  • Mouse Uromodulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 05/24/2017
    - loaded 30 ug of total protein from mouse kidney lysate - wet transfer (100V, 1h) - used buffers as described by datasheet - 1:1500 dilution of UMOD antibody in 0.5% BSA - 1:10000 dilution of rat IgG antibody (HAF005) in 3% BSA
    Mouse Uromodulin Antibody MAB5175

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