Mouse Uromodulin Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Uromodulin by Western Blot.
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Product Details
Citations (6)
Supplemental Products
Reviews (2)

Mouse Uromodulin Antibody Summary

Species Reactivity
Detects mouse Uromodulin in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Uromodulin is observed.
Monoclonal Rat IgG2A Clone # 774056
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Uromodulin
Accession # Q91X17
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Uromodulin antibody by Western Blot. View Larger

Detection of Mouse Uromodulin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Uromodulin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Uromodulin in Mouse Kidney. Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Uromodulin

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.

Entrez Gene IDs
7369 (Human); 22242 (Mouse); 25128 (Rat)
Alternate Names
ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; Tamm-Horsfall glycoprotein; Tamm-Horsfall urinary glycoprotein; THGP; THP; UMOD; uromodulin (uromucoid, Tamm-Horsfall glycoprotein); Uromodulin; Uromucoid

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Citations for Mouse Uromodulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus
    Authors: MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis
    Authors: S Kumar, X Fan, H Milo Rasou, R Sharma, DJ Salant, W Lu
    JCI Insight, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Tolvaptan activates the Nrf2/HO-1 antioxidant pathway through PERK phosphorylation
    Authors: T Fujiki, F Ando, K Murakami, K Isobe, T Mori, K Susa, N Nomura, E Sohara, T Rai, S Uchida
    Sci Rep, 2019;9(1):9245.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
    Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
    Dev. Cell, 2017;41(4):349-365.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle
    PLoS ONE, 2016;11(7):e0158977.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity.
    Authors: Matsumura, Takuhiro, Sugawara, Yo, Yutani, Masahiro, Amatsu, Sho, Yagita, Hideo, Kohda, Tomoko, Fukuoka, Shin-Ich, Nakamura, Yutaka, Fukuda, Shinji, Hase, Koji, Ohno, Hiroshi, Fujinaga, Yukako
    Nat Commun, 2015;6(0):6255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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Reviews for Mouse Uromodulin Antibody

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Mouse Uromodulin Antibody
By Anonymous on 10/25/2021
Application: IF Sample Tested: Kidney tissue Species: Mouse

Mouse Uromodulin Antibody
By Anonymous on 05/24/2017
Application: WB Sample Tested: Kidney tissue Species: Mouse

- loaded 30 ug of total protein from mouse kidney lysate
- wet transfer (100V, 1h)
- used buffers as described by datasheet
- 1:1500 dilution of UMOD antibody in 0.5% BSA
- 1:10000 dilution of rat IgG antibody (HAF005) in 3% BSA