Detection of Mouse Uromodulin by Western Blot.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Uromodulin in Mouse Kidney.
Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105-120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28-148), a ZP domain that mediates oligomerization (aa 335-590), and a cleavable C-terminal propeptide (aa 619-642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601-642, and a 166 aa substitution for aa 441-642. Over aa 25-618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.
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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.
Western Blot: Mouse Uromodulin Antibody [MAB5175].
Other Experimental Details
Other Experimental Details
- loaded 30 ug of total protein from mouse kidney lysate- wet transfer (100V, 1h)- used buffers as described by datasheet- 1:1500 dilution of UMOD antibody in 0.5% BSA- 1:10000 dilution of rat IgG antibody (HAF005) in 3% BSA