Mouse VEGF-D Antibody

Catalog # Availability Size / Price Qty
MAB469
MAB469-SP

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Product Details
Citations (2)
FAQs
Supplemental Products
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Mouse VEGF-D Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGF-D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) VEGF165, rmVEGF-B186, recombinant human (rh) VEGF-C, or rhVEGF-D is observed.
Source
Monoclonal Rat IgG2A Clone # 90409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse VEGF-D
Phe98-Ser206
Accession # P97946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse VEGF-D (Catalog # 469-VD)

Mouse VEGF-D Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse VEGF‑D Biotinylated Antibody (Catalog # BAF469)

Standard: Recombinant Mouse VEGF-D Protein (Catalog # 469-VD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-D

Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1 - 4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF-D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14).

References
  1. Roy, H. et al. (2006) FEBS Lett. 580:2879.
  2. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258.
  3. Orlandini, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11675.
  4. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127.
  5. McColl, B.K. et al. (2003) J. Exp. Med. 198:863.
  6. McColl, B.K. et al. (2007) FASEB J. 21:1088.
  7. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:44307.
  8. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166.
  9. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441.
  10. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708.
  11. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961.
  12. Stacker, S.A. et al. (2001) Nature Med. 7:186.
  13. Karpanen, T. et al. (2006) FASEB J. 20:1462.
  14. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.
Long Name
Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
Entrez Gene IDs
2277 (Human); 14205 (Mouse)
Alternate Names
c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor

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Citations for Mouse VEGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma.
    Authors: Du, Li-Cheng, Chen, Xian-Che, Wang, Dong, Wen, Yan-Jun, Wang, Chun-Tin, Wang, Xue-Mei, Kan, Bing, Wei, Yu-Quan, Zhao, Xia
    Reprod Biol Endocrinol, 2014;12(0):14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
    Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
    Mol. Ther., 2008;16(4):749-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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