Mouse VEGF R1/Flt-1 Antibody

(25 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VEGF R1/Flt-1 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant human VEGF R1 is observed, approximately 4% cross-reactivity with recombinant mouse (rm) VEGF164 is observed, less than 1% cross-reactivity with recombinant rat VEGF164 is observed, and less than 0.2% cross-reactivity with rmVEGF R2 and rmVEGF R3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt-1
    Ser27-Glu759
    Accession # P35969
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse VEGF R1/Flt‑1 Fc Chimera (Catalog # 471-F1)
  • Flow Cytometry
    2.5 µg/106 cells
    bEnd.3 mouse endothelioma cell line
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse PlGF-2 (Catalog # 465-PL) to immobilized Recombinant Mouse VEGF R1/Flt-1 Fc Chimera (Catalog # 471-F1) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Mouse VEGF R1/Flt-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse VEGF R1/Flt‑1 Antibody (Catalog # AF471)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse VEGF R1/Flt‑1 Biotinylated Antibody (Catalog # BAF471)
  • ELISA Standard
     
    Recombinant Mouse VEGF R1/Flt-1 Fc Chimera Protein, CF (Catalog # 471-F1)
  • Neutralization
    Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 5 ng/mL Recombinant Mouse VEGF164.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF R1/Flt‑1 Antibody. Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND50 is typically 2-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R1/Flt-1

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

  • References:
    1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
    2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
  • Long Name:
    Vascular Endothelial Growth Factor Receptor 1
  • Entrez Gene IDs:
    2321 (Human); 14254 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Mechanistic Insights into the Anti-angiogenic Activity of Trypanosoma cruzi Protein 21 and its Potential Impact on the Onset of Chagasic Cardiomyopathy
    Authors: SC Teixeira, DS Lopes, SN Gimenes, TL Teixeira, MS da Silva, RT Brígido, FA da Luz, AA da Silva, MA Silva, PV Florentino, PC Tavares, MA Dos Santos, VM Ávila, MJ Silva, MC Elias, RA Mortara, CV da Silva
    Sci Rep, 2017;7(0):44978.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016;7(0):13650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage.
    Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli
    Sci Rep, 2015;5(0):7890.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  4. Differential apicobasal VEGF signaling at vascular blood-neural barriers.
    Authors: Hudson N, Powner M, Sarker M, Burgoyne T, Campbell M, Ockrim Z, Martinelli R, Futter C, Grant M, Fraser P, Shima D, Greenwood J, Turowski P
    Dev Cell, 2014;30(5):541-52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013;190(7):3500-9.
  6. Nonmuscle myosin light-chain kinase mediates microglial migration induced by HIV Tat: involvement of beta1 integrins.
    Authors: Yao H, Duan M, Yang L, Buch S
    FASEB J, 2013;27(4):1532-48.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  7. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012;484(7392):110-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  8. The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy.
    Authors: Schreurs MP, Houston EM, May V, Cipolla MJ
    FASEB J., 2012;26(1):355-62.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  9. Hypoxia-inducible factor-2alpha regulates GM-CSF-derived soluble vascular endothelial growth factor receptor 1 production from macrophages and inhibits tumor growth and angiogenesis.
    Authors: Roda JM, Sumner LA, Evans R, Phillips GS, Marsh CB, Eubank TD
    J. Immunol., 2011;187(4):1970-6.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells.
    Authors: Shin JY, Yoon IH, Kim JS, Kim B, Park CG
    Cell. Immunol., 2009;256(1):72-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages.
    Authors: Eubank TD, Roberts RD, Khan M, Curry JM, Nuovo GJ, Kuppusamy P, Marsh CB
    Cancer Res., 2009;69(5):2133-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. VEGF promotes vascular sympathetic innervation.
    Authors: Marko SB, Damon DH
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  13. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats.
    Authors: Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, Lennartsson J, Zhu C, Qu Y, Fang C, Hua J, Matsuo O, Fong GH, Ding H, Cao Y, Becker KG, Nash A, Heldin CH, Li X
    J. Clin. Invest., 2008;118(3):913-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
    Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
    Blood, 2007;111(4):1980-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  15. Inhibition of prostate tumor growth and bone remodeling by the vascular targeting agent VEGF121/rGel.
    Authors: Mohamedali KA, Poblenz AT, Sikes CR, Navone NM, Thorpe PE, Darnay BG, Rosenblum MG
    Cancer Res., 2006;66(22):10919-28.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  16. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006;116(2):422-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  17. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
    Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
    Nat. Neurosci., 2006;9(3):340-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  18. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  19. Corneal avascularity is due to soluble VEGF receptor-1.
    Authors: Ambati BK, Nozaki M, Singh N, Takeda A, Jani PD, Suthar T, Albuquerque RJ, Richter E, Sakurai E, Newcomb MT, Kleinman ME, Caldwell RB, Lin Q, Ogura Y, Orecchia A, Samuelson DA, Agnew DW, St Leger J, Green WR, Mahasreshti PJ, Curiel DT, Kwan D, Marsh H, Ikeda S, Leiper LJ, Collinson JM, Bogdanovich S, Khurana TS, Shibuya M, Baldwin ME, Ferrara N, Gerber HP, De Falco S, Witta J, Baffi JZ, Raisler BJ, Ambati J
    Nature, 2006;443(7114):993-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue.
    Authors: Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK
    J. Lab. Clin. Med., 2005;145(4):194-203.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  21. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005;174(1):215-22.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  22. VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
    Authors: Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP
    Invest. Ophthalmol. Vis. Sci., 2004;45(2):368-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  23. Vascular endothelial growth factor-B promotes in vivo angiogenesis.
    Authors: Silvestre JS, Tamarat R, Ebrahimian TG, Le-Roux A, Clergue M, Emmanuel F, Duriez M, Schwartz B, Branellec D, Levy BI
    Circ. Res., 2003;93(2):114-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia.
    Authors: Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C
    J. Cell Biol., 2003;161(6):1163-77.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  25. Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction.
    Authors: Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T
    Blood, 2002;100(7):2487-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 25 Citations
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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