Mouse WISP-1/CCN4 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse WISP‑1/CCN4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse WISP‑1/CCN4
    Thr23-Asn367
    Accession # O54775
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse WISP‑1/CCN4 (Catalog # 1680-WS)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
WISP‑1/CCN4 in Mouse Embryo. WISP‑1/CCN4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Sheep Anti-Mouse WISP‑1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1680) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to developing epithelial cells in duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

  • References:
    1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
    2. Tanada, S. et al. (2001) Oncogene 20:5525.
    3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
    4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
    5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
    6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
    7. SwissProt Accession # O54775.
    8. SwissProt Accession # Q99PP0.
    9. SwissProt Accession # O95388
    10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
    11. Parisi, M.S. et al. (2006) Bone 38:671.
  • Long Name:
    Wnt-1 Induced Secreted Protein 1
  • Entrez Gene IDs:
    8840 (Human); 22402 (Mouse)
  • Alternate Names:
    CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling
    Authors: M Quiros, H Nishio, PA Neumann, D Siuda, JC Brazil, V Azcutia, R Hilgarth, MN O'Leary, V Garcia-Her, G Leoni, M Feng, G Bernal, H Williams, PH Dedhia, C Gerner-Smi, J Spence, CA Parkos, TL Denning, A Nusrat
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
    Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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