Mouse WISP-1/CCN4 Antibody Summary
Accession # O54775
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
WISP‑1/CCN4 in Mouse Embryo. WISP‑1/CCN4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Sheep Anti-Mouse WISP‑1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1680) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to developing epithelial cells in duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).
- Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
- Tanada, S. et al. (2001) Oncogene 20:5525.
- Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
- Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
- Brigstock, D.R. (2003) J. Endocrinol. 178:169.
- Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
- SwissProt Accession # O54775.
- SwissProt Accession # Q99PP0.
- SwissProt Accession # O95388
- French, D.M. et al. (2004) Am. J. Pathol. 165:855.
- Parisi, M.S. et al. (2006) Bone 38:671.
Citations for Mouse WISP-1/CCN4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling
Authors: M Quiros, H Nishio, PA Neumann, D Siuda, JC Brazil, V Azcutia, R Hilgarth, MN O'Leary, V Garcia-Her, G Leoni, M Feng, G Bernal, H Williams, PH Dedhia, C Gerner-Smi, J Spence, CA Parkos, TL Denning, A Nusrat
J. Clin. Invest., 2017;0(0):.
Sample Types: In Vivo
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46.
Sample Types: Tissue Homogenates
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