Detects mouse WISP‑1/CCN4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.
WISP‑1/CCN4 in Mouse Embryo. WISP‑1/CCN4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Sheep Anti-Mouse WISP‑1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1680) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to developing epithelial cells in duodenum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).
Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
Tanada, S. et al. (2001) Oncogene 20:5525.
Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
Brigstock, D.R. (2003) J. Endocrinol. 178:169.
Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
SwissProt Accession # O54775.
SwissProt Accession # Q99PP0.
SwissProt Accession # O95388
French, D.M. et al. (2004) Am. J. Pathol. 165:855.
Parisi, M.S. et al. (2006) Bone 38:671.
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs:
8840 (Human); 22402 (Mouse)
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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