N-Cadherin Antibody (5D5)

Novus Biologicals | Catalog # NBP1-51612

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D5
View all N-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human N-Cadherin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

99.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for N-Cadherin Antibody (5D5)

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.
Western Blot: N-Cadherin Antibody (5D5)BSA Free [NBP1-51612]

Western Blot: N-Cadherin Antibody (5D5)BSA Free [NBP1-51612]

Western Blot: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (5D5) [NBP1-51612] - Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Flow Cytometry: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Flow Cytometry: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) - BSA Free [NBP1-51612]

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human Placenta tissues using CDH2 mouse mAb

Applications for N-Cadherin Antibody (5D5)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200-1:400

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 3 reviews rated 4.7 using NBP1-51612 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N-Cadherin

N-Cadherin, also referred to as Neural Cadherin (NCAD) or Cadherin-2 (CHD2), is a 130 kDa protein that is a member of the calcium-dependent adhesion molecule family of classical (type I) cadherins (1-4). Under the CDH2 gene, human N-cadherin is synthesized as a 906 amino acid protein with a theoretical molecular weight of 99.8 kDa (5). The N-cadherin protein structure is similar to other classical type I cadherins including epithelial (E-) cadherin and placental (P-) cadherin (1,2). N-cadherin consists of a 25 amino acid (aa) N-terminal signal peptide and 134 aa pro-peptide, a 565 aa extracellular domain (ECD) with five cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (1-3,5). The ECD of N-cadherin monomers is responsible for homotypic binding through either cis or trans adhesion (2,3).

N-cadherin is expressed on multiple cell types but is most highly expressed by mesenchymal cells and neural tissue (2). Functionally, N-cadherin has a number of roles including maintaining structural integrity and adhesion, cell signaling, and formation of neuronal synapses and the vascular wall (2). The cytoplasmic tail interacts with beta-catenin which then binds with alpha-catenin, forming the cadherin-catenin adhesion complex, an important component of adhesions junctions (1-3). Given its role in adhesion, N-cadherin serves as an indicator of epithelial-to-mesenchymal transition (EMT) (1-4). The loss of E-cadherin during EMT corresponds with an increase in N-cadherin expression (1-4). This "cadherin-switch" is associated with increased migratory and invasive behavior observed in tumor progress (1-4). Proteases including activity of a disintegrin and metalloprotease 10 (ADAM10), matrix metalloproteinases (MMPs), caspase 3, presenilin, and calpain can cleave N-cadherin as a mechanism for regulating Wnt/beta-catenin signaling and inducing oncogenic signals (3,4). In addition to its expression in solid tumors, N-cadherin has been indicated in hematological disorders such as leukemia and multiple myeloma (1). N-cadherin antagonists are currently being studied as potential therapeutics for a variety of cancer studies (1-2).

References

1. Mrozik, K. M., Blaschuk, O. W., Cheong, C. M., Zannettino, A., & Vandyke, K. (2018). N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. https://doi.org/10.1186/s12885-018-4845-0

2. Loh, C. Y., Chai, J. Y., Tang, T. F., Wong, W. F., Sethi, G., Shanmugam, M. K., Chong, P. P., & Looi, C. Y. (2019). The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells. https://doi.org/10.3390/cells8101118

3. Derycke, L. D., & Bracke, M. E. (2004). N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling. The International Journal of Developmental Biology. https://doi.org/10.1387/ijdb.041793ld

4. Yu, W., Yang, L., Li, T., & Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Frontiers in Oncology. https://doi.org/10.3389/fonc.2019.00989

5. Unitprot (P1903)

Long Name

Neural Cadherin

Alternate Names

Cadherin-2, CD325, CDH2, NCadherin

Entrez Gene IDs

12558 (Mouse); 83501 (Rat)

Gene Symbol

CDH2

UniProt

P19022

Additional N-Cadherin Products

Product Documents for N-Cadherin Antibody (5D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for N-Cadherin Antibody (5D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for N-Cadherin Antibody (5D5)

Customer Reviews for N-Cadherin Antibody (5D5) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • N-Cadherin in MCF7
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Blot of whole lysate of MCF7 cells
    Used at 1 ug/mL
    N-Cadherin Antibody (5D5) NBP1-51612
  • N-cadherin in adipocyte coculture
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipocytes HCT116 coculture
    Species: Human
    Verified Customer | Posted 07/18/2025
    N-Cadherin band
    Co culture of adipocytes and HCT116 cells. 1:2000 antibody dilution
    N-Cadherin Antibody (5D5) NBP1-51612
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 10/04/2015

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Protocols

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FAQs

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