NKX2.5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31558

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (93%), Canine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NKX2.5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NKX2.5. The exact sequence is proprietary.

Localization

Nucleus

Marker

Cardiac Progenitor Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NKX2.5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-31558]

Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-31558]

Immunocytochemistry/Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558]

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558]

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558] - Mouse heart. Nkx2.5 stained by Nkx2.5 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558]

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558]

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558] - Rat heart. Nkx2.5 stained by Nkx2.5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
NKX2.5 Antibody

Western Blot: NKX2.5 Antibody [NBP1-31558] -

Western Blot: NKX2.5 Antibody [NBP1-31558] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NKX2.5 Antibody

Western Blot: NKX2.5 Antibody [NBP1-31558] -

Western Blot: NKX2.5 Antibody [NBP1-31558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NKX2.5 Antibody

Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-31558] -

Immunocytochemistry/ Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - NKX2.5 antibody detects NKX2.5 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NKX2.5 stained by NKX2.5 antibody (NBP1-31558) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
NKX2.5 Antibody - BSA Free

Western Blot: NKX2.5 Antibody [NBP1-31558] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated withand does not endorse this product.
NKX2.5 Antibody - BSA Free

Western Blot: NKX2.5 Antibody [NBP1-31558] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NKX2.5 antibody (NBP1-31558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for NKX2.5 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

Reported in scientific literature (PMID: 26612359)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NKX2.5

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Long Name

NK2 Homeobox 5

Alternate Names

CSX1, NKX2-5, NKX2E

Gene Symbol

NKX2-5

Additional NKX2.5 Products

Product Documents for NKX2.5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NKX2.5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NKX2.5 Antibody - BSA Free

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Protocols

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FAQs

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