p53 Antibody (PAb 240) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80898

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Xenopus (Negative), Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAb 240

Format

Azide and BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).

Epitope

The epitope has been mapped between amino acids 213 and 217 on human p53.

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 8710879)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p53 Antibody (PAb 240) - Azide and BSA Free

Western Blot: p53 Antibody (PAb 240)Azide and BSA Free [NBP2-80898]

Western Blot: p53 Antibody (PAb 240)Azide and BSA Free [NBP2-80898]

Western Blot: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Immunohistochemistry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Immunohistochemistry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detect
Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898]

Flow Cytometry: p53 Antibody (PAb 240) - Azide and BSA Free [NBP2-80898] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Applications for p53 Antibody (PAb 240) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

5 ug/ml. Use reported in scientific literature (PMID 1394225)

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Frozen

reported in multiple pieces of scientific literature

Immunohistochemistry-Paraffin

1:250-1:500

Immunoprecipitation

10ug/mg

Western Blot

1:1000-1:2000
Application Notes
Antigen retrieval with IHC-P is not essential but may optimize staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. This antibody is CyTOF ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (PAb 240) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p53 Antibody (PAb 240) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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