p53 Antibody (PAb 240) - BSA Free

Novus Biologicals | Catalog # NB200-103

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Xenopus (Negative), Yeast

Cited:

Human, Mouse, Rat, Yeast

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Hydrolysis Assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAb 240

Format

BSA Free
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).

Epitope

The epitope has been mapped between amino acids 213 and 217 on human p53. The epitiope may be conformational as it reacts with mutant p53 in nondenatured form.

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 8710879)

Localization

Nuclear/cytoplasmic

Specificity

This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions. It does not recognize nondenatured wild type p53. In nondenaturing conditions (IP) it only recognizes mutant p53.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p53 Antibody (PAb 240) - BSA Free

Western Blot: p53 Antibody (PAb 240)BSA Free [NB200-103]

Western Blot: p53 Antibody (PAb 240)BSA Free [NB200-103]

Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow (Intracellular): p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow (Intracellular): p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) - BSA Free [NB200-103]

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on Neuro2a cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Applications for p53 Antibody (PAb 240) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

5 ug/ml. Use reported in scientific literature (PMID 1394225)

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Frozen

reported in multiple pieces of scientific literature

Immunohistochemistry-Paraffin

1:250-1:500

Immunoprecipitation

10ug/mg

Western Blot

1:1000-1:2000
Application Notes
Antigen retrieval with IHC-P is not essential but may optimize staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. This antibody is CyTOF ready.

Reviewed Applications

Read 3 reviews rated 4.3 using NB200-103 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (PAb 240) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p53 Antibody (PAb 240) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p53 Antibody (PAb 240) - BSA Free

Customer Reviews for p53 Antibody (PAb 240) - BSA Free (3)

4.3 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • p53 Antibody (PAb 240)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: C166 mouse cell line
    Species: Mouse
    Verified Customer | Posted 09/14/2018
    25µg of C166 whole cell lysate. Right lane Nutlin 3A treated sample which leads to induction of p53. Left lane DMSO treated control.
    p53 Antibody (PAb 240) - BSA Free NB200-103
  • Name: Matt Stratton
    Application: Western Blot
    Sample Tested:
    Species: Rat
    Verified Customer | Posted 03/26/2014
  • Name: Astrid Spannhoff
    Application: Western Blot
    Sample Tested: Whole Cell Lysate of MCF-7 and HeLa cells, Sample Amount: 10 ul
    Species: Human
    Verified Customer | Posted 10/13/2010
    p53 Antibody (PAb 240) - BSA Free NB200-103

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